2017
DOI: 10.1186/s13071-017-2185-6
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Characterising PvRBSA: an exclusive protein from Plasmodium species infecting reticulocytes

Abstract: Background Plasmodium vivax uses multiple ligand-receptor interactions for preferential invasion of human reticulocytes. Several of these ligands have been identified by in silico approaches based on the role displayed by their orthologs in other Plasmodium species during initial adhesion or invasion. However, the cell adhesion role of proteins that are exclusive to species that specifically invade reticulocytes (as P. vivax and P. cynomolgi) has not been evaluated to date. This study aimed to characterise an … Show more

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Cited by 13 publications
(21 citation statements)
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“…One antigen shared just by P. vivax and P. cynomolgi (both having reticulocyte predilection) has been characterized recently ( Moreno-Perez et al, 2017 ). This antigen (named reticulocyte binding surface antigen – RBSA) is encoded by a two-exon gene ( Moreno-Perez et al, 2017 ) located on Chr 3. The protein product has a signal peptide and two transmembrane helices and is located on mature Mrz membrane.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…One antigen shared just by P. vivax and P. cynomolgi (both having reticulocyte predilection) has been characterized recently ( Moreno-Perez et al, 2017 ). This antigen (named reticulocyte binding surface antigen – RBSA) is encoded by a two-exon gene ( Moreno-Perez et al, 2017 ) located on Chr 3. The protein product has a signal peptide and two transmembrane helices and is located on mature Mrz membrane.…”
Section: Introductionmentioning
confidence: 99%
“…This protein seems to be involved in Mrz invasion, since recombinant P. vivax RBSA (rPvRBSA) binds to a subpopulation of immature reticulocytes having a Duffy positive phenotype; it is also recognized by the human immune system in natural infections. It has thus been suggested as a putative vaccine candidate for anti- P. vivax malaria vaccine development ( Moreno-Perez et al, 2017 ).…”
Section: Introductionmentioning
confidence: 99%
“…described protocols(Arevalo-Pinzon et al, 2017;Moreno-Perez, Baquero, Chitiva-Ardila, & Patarroyo, 2017).Briefly, or PvRON2-RI recombinant protein were incubated with 5% UCB RBC and then incubated with antibodies against the transferrin receptor (CD71) on reticulocytes, against cluster of differentiation 45 (CD45) in leukocytes (some activated leukocytes are CD71 + , but reticulocytes are CD45 − , so the reticulocyte population selected was CD71 + CD45 − ) and antibodies against the 6X-His-tag in recombinant proteins. UCB RBC were preincubated with peptides before adding recombinant protein for competition assays with peptides 40305 and 40595.…”
mentioning
confidence: 99%
“…Protein expression was induced by adding 0.2% L-arabinose and incubating at 37 • C for 4 h at 250 rpm. The culture was spun at 4,500 rpm for 20 min; the pellet was then suspended in solubilising buffer (Moreno-Perez et al, 2017), lysozyme and a protease inhibitor cocktail (Thermo Fisher), as previously described. This mixture was incubated overnight at 10 rpm using a tube rotator (Fisher Scientific, Waltham, USA).…”
Section: Expressing and Obtaining Rpvceltosmentioning
confidence: 99%