Tuckeroo (Cupaniopsis anacardioides) is an Australian native tree, possessing high level bioactivity and antioxidant activity. To prevent deterioration of active constituents, appropriate drying practices must be determined. This study comparatively evaluates the impact of a range of drying methods including freeze-, microwave-, vacuum-, hot air-and sun-drying on the physical, phytochemical and antioxidant characteristics of Tuckeroo fruit. Experimental results showed that the five drying methods had significant impact on the physicochemical properties and antioxidant activity of the fruits. Of the drying methods assessed, freeze drying best preserved Tuckeroo activity, recording higher total phenolic content (TPC) (81.88 mg gallic acid equivalent (GAE)/g), total flavonoids (TFC) (107.71 mg catechin equivalent (CAE)/g), proanthocyanidins (TPro) (83.86 mg CAE/g) and exhibited the strongest antioxidant capacity. However, vacuum drying at 65 kPa, 100 • C for 5 h is recommended for drying Tuckeroo fruits for further processing in a large scale as it also retained high levels of TPC, TFC and TPro (58 mg GAE/g, 91 mg CAE/g and 74 mg CAE/g, respectively).An important step in phytochemical profiling is sample preservation, as it is important that the bioactive constituents present in the sample are well preserved and not subject to denaturing [10,11]. Drying is considered an appropriate preservation technique for a wide variety of herbs or fruits, where short shelf-life, spoilage or short seasonality are concerns. The choice of drying method is an important consideration as drying conditions can have a significant impact on the preservation of biological and antioxidant activity. For example, thermal drying methods (microwave-, oven-and sun-drying) are reported to adversely affect total phenolic content (TPC) and antioxidant activity in the leaves of Alpinia zerumbet, Etlingera elatior, Curcuma longa and Kaempferia galanga [12]. By contrast, Dewanto, Wu, Adom and Liu [13] reported the antioxidant activity of tomato increased after heat treatment at 88 • C within 2, 15 and 30 min. A similar trend was found for sweet corn under the commercial processing conditions [14], Shiitake mushroom (Lentinus edodes) [15] and ginseng [16]. Additionally, microwave drying was illustrated as the best dehydration method for xao tam phan (Paramignya trimera) roots [10] or for the banana peels [17], whereas vacuum-and hot air-drying were the most suitable for lemon (Citrus limon) pomace drying [18], suggesting that the mechanics of the drying process itself impacts differently depending on the presence or absence of direct irradiation, temperature, the physical structure of the plant material itself and the characteristics of the chemical constituents. Therefore, investigation on the impact of different drying methods and conditions for Tuckeroo fruits is needed.This study aimed to assess the impact of five different drying techniques including freeze drying, sun drying as well as microwave drying, vacuum drying and hot air drying with diff...