Characteristic Dihydroagarofuran Sesquiterpenoids with Neuroprotective Effects from the Celastraceae Plant Tripterygium wilfordii

Abstract

Main observation and conclusion Ten new characteristic dihydroagarofuran sesquiterpenoids and three known ones were acquired from the Celastraceae plant Tripterygium wilfordii. Their structure characterizations were done through spectroscopic data, while compounds 10—12 were further confirmed by single crystal diffractions. Several dihydroagarofurans protected PC12 cells against H2O2‐induced cytotoxicity as detected by CCK8 assay. Compounds 5—7 and 13 significantly increased the levels of reactive oxygen species (ROS) and superoxide dismutase (SOD), and decreased malondialdehyde (MDA) levels. In addition, compounds 5—7 and 13 activated the Nrf2 signaling pathway and increased Nrf2, NQO1, and HO1 expression levels in PC12 cells after H2O2 treatment.

Two New Eudesmane‐Type Sesquiterpene from Clonostachys sp. Y6‐1and Their Cytotoxic Activity

“…The MDA‐MB‐231 cell lines and SW620 cell lines were purchased from the DSMZ cell bank. Cells viability was analyzed using a CCK‐8 assay kit [19] . The four cell lines (5×10 3 cells per well) were added to 96‐well plates and cultured with the appropriate media (100 μL/well) for 24 h. All compounds were dissolved in dimethyl sulfoxide (DMSO) and then diluted with the cell culture medium to the desired concentrations (0, 4, 8, 16, 32, and 64 μM).…”

“…Cells viability was analyzed using a CCK-8 assay kit. [19] The four cell lines (5×10 3 cells per well) were added to 96-well plates and cultured with the appropriate media (100 μL/well) for 24 h. All compounds were dissolved in dimethyl sulfoxide (DMSO) and then diluted with the cell culture medium to the desired concentrations (0, 4, 8, 16, 32, and 64 μM). The cells were treated with varying concentrations of compounds for 24 h (37 °C, 5 % CO 2 ).…”

Natural Sesquiterpenoids, Diterpenoids, Sesterterpenoids, and Triterpenoids with Intriguing Structures from 2017 to 2022

“…As one of the largest classes and the most frequently reported terpenoids, sesquiterpenoids, derived from FPP, have attracted increasing attention from organic chemists and pharmacologists due to their diverse carbon skeletons as exemplified by various ring systems and polymer architectures and extensive biological activities including neuroprotective, anti-inflammatory, and antimicrobial effects. [8][9][10][11][12][13][14][15] Fig. 2 .…”

“…A pair of caged norsesquiterpenoidal enantiomers (±)-preuisolactone A (±39 ), featuring an unprecedented tricyclo[4.4.0 1,6 .0 2,8 ]decane carbon scaffold determined by X-ray crystallography, have been found from the plant endophytic fungus preussia isomera . [60][61][62] A plausible biosynthetic pathway for (+-)-39 has been proposed (Fig.…”

Natural Sesquiterpenoids, Diterpenoids, Sesterterpenoids, and Triterpenoids with Intriguing Structures from 2017 to 2022

“…As one of the largest classes and the most frequently reported terpenoids, sesquiterpenoids, derived from FPP, have attracted increasing attention from organic chemists and pharmacologists due to their diverse carbon skeletons as exemplified by various ring systems and polymer architectures and extensive biological activities including neuroprotective, anti‐inflammatory, antimicrobial effects and the treatment of AD. [ 13‐21 ]…”