Characteristic fragmentation patterns of the trimethylsilyl and trimethylsilyl–oxime derivatives of various saccharides as obtained by gas chromatography coupled to ion-trap mass spectrometry

Füzfai, Zs.; Boldizsár, Imre; Molnár-Perl, I.

doi:10.1016/j.chroma.2007.11.023

Cited by 42 publications

(25 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the analysis of free metabolites, the fragment m/z 361 of the Suc trimethylsilyl derivative was monitored. This abundant ion results from fragmentation at the glycosidic linkage, leading to m/z 361 (C 15 H 33 O 4 Si 3 ) representing all six carbons of the glucosyl moiety of the Suc molecule (Fuzfai et al, 2008). Mass spectral data were extracted using the ChemStation Program (MSD ChemStation; Agilent Technologies).…”

Section: Gc-ms Analysis Of Labeling Profilesmentioning

confidence: 99%

Analysis of Metabolic Flux Phenotypes for Two Arabidopsis Mutants with Severe Impairment in Seed Storage Lipid Synthesis

2009

View full text Add to dashboard Cite

Major storage reserves of Arabidopsis (Arabidopsis thaliana) seeds are triacylglycerols (seed oils) and proteins. Seed oil content is severely reduced for the regulatory mutant wrinkled1 (wri1-1; At3g54320) and for a double mutant in two isoforms of plastidic pyruvate kinase (pkpb 1 pkpa; At5g52920 and At3g22960). Both already biochemically well-characterized mutants were now studied by 13 C metabolic flux analysis of cultured developing embryos based on comparison with their respective genetic wild-type backgrounds. For both mutations, in seeds as well as in cultured embryos, the oil fraction was strongly reduced while the fractions of proteins and free metabolites increased. Flux analysis in cultured embryos revealed changes in nutrient uptakes and fluxes into biomass as well as an increase in tricarboxylic acid cycle activity for both mutations. While in both wild types plastidic pyruvate kinase (PK p ) provides most of the pyruvate for plastidic fatty acid synthesis, the flux through PK p is reduced in pkpb 1 pkpa by 43% of the wild-type value. In wri1-1, PK p flux is even more reduced (by 82%), although the genes PKpb 1 and PKpa are still expressed. Along a common paradigm of metabolic control theory, it is hypothesized that a large reduction in PK p enzyme activity in pkpb 1 pkpa has less effect on PK p flux than multiple smaller reductions in glycolytic enzymes in wri1-1. In addition, only in the wri1-1 mutant is the large reduction in PK p flux compensated in part by an increased import of cytosolic pyruvate and by plastidic malic enzyme. No such limited compensatory bypass could be observed in pkpb 1 pkpa.For many field crops, seeds are the most important part used for human and animal nutrition. Seed storage compounds such as triacylglycerol, proteins, and carbohydrates typically make up most of the mass of mature seeds, and the proportions of these components have large species-specific variations. Since seed composition and yield are important traits for breeding and agricultural research, partitioning of carbon and nitrogen into the major storage products within the developing seed is an important process. Based on knowledge of the biochemical pathways that convert maternal carbon and nitrogen supplies into storage products (Baud and Lepiniec, 2008), flux analysis with cultured developing seeds gave important insights into in vivo pathway usage and the integrated function and regulation of metabolic pathways in storage synthesis (Schwender and Ohlrogge, 2002;

show abstract

Section: Gc-ms Analysis Of Labeling Profilesmentioning

confidence: 99%

Analysis of Metabolic Flux Phenotypes for Two Arabidopsis Mutants with Severe Impairment in Seed Storage Lipid Synthesis

2009

View full text Add to dashboard Cite

show abstract

“…GC-MS is the most widely applied technique for the characterization of organic aerosols since it yields excellent results in terms of separation, sensitivity and number of species analyzed in the identification and quantification of many compounds [1,[3][4][5]10,12,13,17,19,[23][24][25][26][27][28][29][30][31]. Indeed, multi-residue analytical methodologies, that achieve simultaneous analysis of several compounds, are useful for reducing the complexity and time required for sample preparation and analysis in order to provide a large amount of reliable data on molecular tracers in atmospheric PM samples [1,[3][4][5]12].…”

Section: Introductionmentioning

confidence: 99%

“…The data for the representative target analytes are reported in bold XLOD (5th column) computed as XLOD = 6 b /b1, where b1 is the slope of the calibration line, XLOD*, (6th column) computed as the concentration corresponding to three times the standard deviation of the peak areas generated by blank filters. (N,O-bis(trimethylsilyl)-trifluoroacetamide) as silylation reagent has been widely used to analyze polyacids and sugars/sugar alcohols in PM samples, since it provides the low detection limits (≤2 ng m −3 ) and high reproducibility required by environmental monitoring [1,3,4,12,18,23,[25][26][27][28][29][30][31]. The silylation conditions reported by various authors widely differ, and at times are even contradictory, because these procedures usually focus on analysis of a particular class of compounds, i.e., carboxylic acids or sugars [1][2][3][4][15][16][17][18][19]23,24].…”

Section: Introductionmentioning

confidence: 99%

GC–MS analysis of water-soluble organics in atmospheric aerosol: Response surface methodology for optimizing silyl-derivatization for simultaneous analysis of carboxylic acids and sugars

Pietrogrande¹,

Bacco²

2011

Analytica Chimica Acta

View full text Add to dashboard Cite

“…Given that our intention was not to identify individual simple sugar components, the analysis is further done by selecting specific abundant fragment ions of simple sugars. By selecting ions 204 and 217m/z, characteristics of TMS derivatives of monosaccharides (pyranose and furanose ring, respectively) (Karady and Pines 1970;Ačanski and Vujić 2014), and 361m/z, characteristic abundant fragment ion of TMS derivatives of di-and trisaccharides (Füzfai et al 2008), the selected ion monitoring (SIM) chromatograms were obtained from complex TIC chromatograms, which simplified the detection procedure of simple sugar derivative peaks in the chromatograms.…”

Section: Resultsmentioning

confidence: 99%

Rapid Method for Small Grain and Corn Flour Authentication Using GC/EI–MS and Multivariate Analysis

et al. 2015

View full text Add to dashboard Cite

The aim of this study was the application of the gas chromatography-mass spectrometry system (GC/EI-MS) system and multivariate data analysis to investigate the possibility of chemical differentiation between small grain flour (wheat, barley, oat, triticale, rye) and corn flour samples. All cereal flour samples were first defatted with hexane, after which the extraction with ethanol was performed. Extracted simple sugars (monosaccharides, disaccharides, trisaccharides, and sugar alcohols) were analyzed in the form of their corresponding trimethylsilyl oximes. Peaks of simple sugar derivatives were selected in total ion current (TIC) chromatograms by monitoring exclusively the following characteristic abundant ions: 204, 217, and 361m/z. The total surface areas under the selected peaks were subjected to multivariate analysis. Applying principal coordinate analysis and hierarchical cluster analysis to obtained data, samples of corn flour could be very clearly distinguished from all samples of small grain flour, which presented a weaker separation among each other. This method circumvents common analytical procedures by excluding simple sugar identifications, quantitative analysis, the use of analytical standards, and calibration curves. Results are applicable in the quality assurance of mixed flour on the market, considering the increased popularity of their consumption in human nutrition.

show abstract

Characteristic fragmentation patterns of the trimethylsilyl and trimethylsilyl–oxime derivatives of various saccharides as obtained by gas chromatography coupled to ion-trap mass spectrometry

Cited by 42 publications

References 26 publications

Analysis of Metabolic Flux Phenotypes for Two Arabidopsis Mutants with Severe Impairment in Seed Storage Lipid Synthesis

Analysis of Metabolic Flux Phenotypes for Two Arabidopsis Mutants with Severe Impairment in Seed Storage Lipid Synthesis

GC–MS analysis of water-soluble organics in atmospheric aerosol: Response surface methodology for optimizing silyl-derivatization for simultaneous analysis of carboxylic acids and sugars

Rapid Method for Small Grain and Corn Flour Authentication Using GC/EI–MS and Multivariate Analysis

Contact Info

Product

Resources

About