Characteristic genotypes discriminate between Babesia canis isolates of differing vector specificity and pathogenicity to dogs

Zahler, Monika; Schein, E.; Rinder, Heinz; Gothe, R.

doi:10.1007/s004360050445

Cited by 162 publications

(120 citation statements)

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“…Scrutiny of the results of microscopy and PCR analysis of individual blood sample in the present study revealed that out of 39, 5 microscopic positive B. gibsoni cases remained negative in PCR and the false positivity was 12.82 %. Confirming the vector specific geographic distribution (Uilenberg et al 1989;Zahler et al 1998) of the subspecies of B. canis, several workers reported significant association of Dermacentor reticulatus with B. canis canis prevalent in Europe (Caccio et al 2002;Porchet et al 2007;Zygner et al 2008;Welc-Faleciak et al 2009) and Rhipicephalus sanguineus with B. canis vogeli in tropical and subtropical countries (O'Dwyer et al 2009;Gotsch et al 2009). Using PIRO-A1 and PIRO-B primers Foldvari et al (2005) reported Babesia DNA having 99.8 or 100 % similarity with B. canis canis in sequencing.…”

Section: Resultsmentioning

confidence: 99%

Babesia infection in naturally exposed pet dogs from a north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction

Laha

Bhattacharjee²,

Sarmah³

et al. 2013

J Parasit Dis

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The objective of the study was to detect Babesia infections in pet dogs of a north-eastern state of India. The diagnostic efficacy of Babesia infection by polymerase chain reaction (PCR) technique has been compared with microscopy examination. For this, a total of 111 blood samples of pet dogs presented at clinical complex of the College of Veterinary Science, Guwahati, Assam with clinical signs suspected for Babesia infection subjected to the study. A total of 44 (39.63 %) dogs were diagnosed as positive for Babesia infections after microscopic examination. Among these, Babesia canis infection was diagnosed in 5 dogs (4.50 %) and B. gibsoni infection in 39 (35.13 %) dogs microscopically in Giemsa stained blood smears. Molecular diagnosis using PCR detected 63 (56.75 %) dogs positive for Babesia infection. Single infection with B. canis was found in 9 (8.10 %) dogs while B. gibsoni alone was detected in 3 (2.70 %) dogs. Mixed infections by both these species were detected in 51 (45.94 %) dogs. Overall, PCR detected 54 (48.64 %) dogs as B. gibsoni and 60 (54.05 %) dogs as B. canis positive.

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Section: Resultsmentioning

confidence: 99%

Babesia infection in naturally exposed pet dogs from a north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction

Laha

Bhattacharjee²,

Sarmah³

et al. 2013

J Parasit Dis

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“…However, genotypic analysis may provide useful indicators to decide for or against possible conspecificity of the unknown Pallas's cat piroplasm to another piroplasm since cross-breeding experiments to show genetic exchange between parasites is not possible (Zahler et al, 1998). Sequence comparison of the conserved 18S rRNA gene allows examination of phylogenetically distant taxa.…”

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confidence: 99%

“…A second indicator of support against possible conspecificity is the absolute level of genetic differentiation between the unknown Pallas's cat piroplasm in comparison with other related parasites for which species status in accepted (Zahler et al, 1998;Kjemtrup. et al, 2000).…”

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confidence: 99%

An Intraerythrocytic Small Piroplasm In Wild-caught Pallas's Cats (Otocolobus manul) from Mongolia

Ketz-Riley

Reichard

Bussche

et al. 2003

Journal of Wildlife Diseases

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During the quarantine examination of four Pallas's cats (Otocolobus manul) imported from Mongolia in October and December 2000, intraerythrocytic piroplasms were detected on Wright-Giemsa stained blood films that were morphologically indistinguishable from other small piroplasms of felids. Further characterization of this unknown organism via polymerase chain reaction amplification, sequencing of a portion of the 18S nuclear small subunit rRNA gene, and comparisons with orthologous sequences from other piroplasms, revealed similarity to Cytauxzoon felis. This is the first report of naturally occurring erythroparasitemia in Pallas's cats and the first documented case of naturally occurring piroplasm infections in a free-ranging felid from Mongolia.

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“…On the basis of vector specificity and antigenic properties, B. canis has been subdivided into three subspecies: B. canis canis, B. canis vogeli and B. canis rossi (UILENBERG et al, 1989). Nevertheless, other researchers have adopted phylogenetic criteria to support the taxonomic classification for these organisms into three distinct species (ZAHLER et al, 1998). The red dog tick Rhipicephalus sanguineus, which is recognized as the tick vector of B. gibsoni and B. canis vogeli, is widespread in Brazil, especially in urban areas (SZABÓ et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil

Duarte

Parente

Pereira

et al. 2011

Rev. Bras. Parasitol. Vet.

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The genus Babesia comprises protozoa that cause diseases known as babesiosis. Dogs are commonly affected by Babesia canis or Babesia gibsoni. Babesia canis is divided into the subspecies Babesia canis canis, Babesia canis vogeli and Babesia canis rossi. Among these, Babesia canis vogeli predominates in Brazil. The objective of this study was to conduct a phylogenetic analysis on Babesia isolates from dogs in Goiânia, Goiás. Blood samples were obtained from 890 dogs presenting clinical signs suggestive of canine babesiosis that were attended at a veterinary hospital of Goiás. Only samples presenting typical intraerythrocytic parasites were used in the study. These were subjected to DNA extraction and amplification of a fragment of the 18S rRNA, by means of PCR. The PCR products were purified and sequenced. Sequences were obtained from 35 samples but only 17 of these were kept after quality assessment. Similarity analysis using BLASTn demonstrated that all 17 sequences corresponded to B. canis vogeli. Analysis using the Mega4 software showed that the isolates of B. canis vogeli from dogs in Goiânia present a high degree of molecular similarity (99.2 to 100%) in comparison with other reference isolates from other regions of Brazil and worldwide, deposited in GenBank.Keywords: Babesia canis vogeli, canine babesiosis, molecular characterization, phylogeny. Resumo

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Characteristic genotypes discriminate between Babesia canis isolates of differing vector specificity and pathogenicity to dogs

Cited by 162 publications

References 10 publications

Babesia infection in naturally exposed pet dogs from a north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction

Babesia infection in naturally exposed pet dogs from a north-eastern state (Assam) of India: detection by microscopy and polymerase chain reaction

An Intraerythrocytic Small Piroplasm In Wild-caught Pallas's Cats (Otocolobus manul) from Mongolia

Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil

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