Characteristic infrared spectroscopic patterns in the protein bands of human breast cancer tissue

Eckel, Rainer; Huo, Hong; Guan, Hongwei; Hu, Xiang; Che, Xun; Huang, Weidong

doi:10.1016/s0924-2031(01)00134-5

Cited by 125 publications

(59 citation statements)

References 18 publications

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“…The peak of 1655 cm À1 is attributed to the absorption of the CQO stretching vibration coupled to the in-phase bending of the N-H bond which may represent certain kind of a helix structure inside [7]. The peak at 1543 cm À1 may represent that there is larger anti-parallel b-sheet structural absorptions in malignant tissue but smaller in normal and benign tissues [3]. The double peak at 1464 and 1474 cm À1 can be clearly observed in SR-FTIR but not in conventional IR experiments, because the resolving power is low in the latter method and the double peak cannot be resolved.…”

Section: Resultsmentioning

confidence: 99%

“…To enhance the early detection rate, much research work has been done in the last years by using spectroscopic methods [2,3], which are mostly based on compact spectrometers focusing on UV excitation with the purpose to reach a relative high fluorescence yield. In addition, the laser spectroscopy provides a powerful and sensitive approach to reveal changes in the structural and biochemical properties that occur in healthy and abnormal cells in tissues [4].…”

Section: Introductionmentioning

confidence: 99%

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Infrared absorption of human breast tissues in vitro

Liu

Zhang

Yan

et al. 2006

Journal of Luminescence

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Infrared absorption of human breast tissues in vitro

Liu

Zhang

Yan

et al. 2006

Journal of Luminescence

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“…Several studies have reported the potential of vibrational spectroscopies, IR absorption and Raman scattering to characterize biological tissues. In the last few years, investigations have been carried out to differentiate cancerous tissue from benign tissues in sections of the colon (Argov et al 2002;Lasch et al 2004;Kondepati et al 2007), cervix , stomach (Ling et al 2002;Park et al 2007), breast (Eckel et al 2001), skin (Tfayli et al 2005), bowel (Argov et al 2004), ovary (Krishna et al 2005) and the mouth (Krishna et al 2004). A dedicated IR or Raman probe has been used in vivo studies for the detection of colorectal cancer (Li et al 2005), and Barrett's epithelium in rat esophagus (Boere et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Fourier transform infrared microspectroscopy as a diagnostic tool for distinguishing between normal and malignant human gastric tissue

2011

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Fourier transform infrared (FTIR) microspectroscopy can be considered to be a fast and non-invasive tool for distinguishing between normal and cancerous cells and tissues without the need for laborious and invasive sampling procedures. Gastric samples from four patients (age, 65±2 years) were analysed. Samples were obtained from the organs removed during gastrectomy and then classified as normal or cancerous. Classification was based on histopathological examinations at our institution. Formalin-fixed sections of gastric tissue were analysed by FTIR-microspectroscopy. To characterize differences between sections of normal and cancerous tissue, specific regions of the spectra were analysed to study variations in the levels of metabolites. To distinguish between two conditions (normal and cancerous), changes in the relative intensity of bands in the range 600-4000 cm⁻¹ were analysed. A FTIR spectral map of the bands in the region 2800-3100 cm⁻¹ and 900-1800 cm⁻¹ were created to analyse pathological changes in tissues. The limited data available showed that normal gastric tissue had stronger absorption than cancerous tissue over a wide region in the four patients. There was a significant decrease in total biomolecular components for cancerous tissue compared with normal tissue.

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“…3), which is sensitive to secondary structure of proteins, demonstrate that the amide I absorption bands are larger in the double drawn fiber and has a clear shoulder in the 1670-1715 region. This shoulder may be due to high frequency vibrations of an antiparallel β sheets of amide I (Eckel, 2001). This analysis may suggest that uncoiling of the coiled-coil dimer has taken place concurrently with transition of the secondary structure to exhibit a more uniform surface.…”

Section: The Structural Organization Of Ce-lamin Macroscopic Fibersmentioning

confidence: 91%

The assembly of C. elegans lamins into macroscopic fibers

Zingerman-Koladko

Khayat

Harapin

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

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Intermediate filament (IF) proteins are known mainly by their propensity to form viscoelastic filamentous networks within cells. In addition, IF-proteins are essential parts of various biological materials, such as horn and hagfish slime threads, which exhibit a range of mechanical properties from hard to elastic. These properties and their self-assembly nature made IF-proteins attractive building blocks for biomimetic and biological materials in diverse applications. Here we show that a type V IF-protein, the Caenorhabditis elegans nuclear lamin (Ce-lamin), is a promising building block for protein-based fibers. Electron cryo-tomography of vitrified sections enabled us to depict the higher ordered assembly of the Ce-lamin into macroscopic fibers through the creation of paracrystalline fibers, which are prominent in vitro structures of lamins. The lamin fibers respond to tensile force as other IF-protein-based fibers, i.e., hagfish slime threads, and possess unique mechanical properties that may potentially be used in certain applications. The self-assembly nature of lamin proteins into a filamentous structure, which is further assembled into a complex network, can be easily modulated. This knowledge may lead to a better understanding of the relationship in IF-proteins-based fibers and materials, between their hierarchical structures and their mechanical properties.

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Characteristic infrared spectroscopic patterns in the protein bands of human breast cancer tissue

Cited by 125 publications

References 18 publications

Infrared absorption of human breast tissues in vitro

Infrared absorption of human breast tissues in vitro

Fourier transform infrared microspectroscopy as a diagnostic tool for distinguishing between normal and malignant human gastric tissue

The assembly of C. elegans lamins into macroscopic fibers

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