Characteristic MALDI-MS lipid profiles of Gir, Holstein and crossbred (Gir x Holstein) oocytes recovered by ovum pick-up

Marsico, Thamiris Vieira; Sales, J. N. S.; Ferreira, Christina R.; Sudano, Mateus José; Viana, J. H. M.; Camargo, Luiz Sérgio de Almeida; Eberlin, Marcos N.; Seneda, Marcelo Marcondes; Baruselli, Pietro Sampaio

doi:10.1016/j.livsci.2020.104380

Cited by 5 publications

(3 citation statements)

References 66 publications

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“…Therefore, cross-breeding is an alternative to using complementarity and heterosis. Consistent with herd performance improvement, cross-breeding resulted in variation (including reproductive traits) among individuals (Marsico et al 2021). Jemal et al (2021) reported that superovulation response was higher in crossbreds (Bos taurus x Bos indicus).…”

Section: Introductionmentioning

confidence: 91%

“…Since Bos taurus was naturally selected under cold temperatures, this could affect oocyte and lipid composition in the embryo (Sudano et al 2012). Marsico et al (2021) demonstrated that triacylglycerol predominates in Bos taurus embryos. Triacylglycerol forms lipid droplets in the cytoplasm and serves as an energy source and cell signaling pathway for mammalian embryonic development.…”

Section: Embryo Qualitymentioning

confidence: 99%

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Embryo Production and Development from Superovulated Donors in Double-Muscled Cattle and Their Crosses

Irma,

Rasad,

Hilmia

et al. 2023

JITV

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Belgian Blue was introduced in Indonesia to increase the biodiversity of livestock genetic resources. Â Belgian Blue was crossed with Ongole grade to increase the productivity of local cattle.Â Therefore, this study evaluates reproduction traits, especially the response to superovulation, embryonic development, and quality of Belgian Blue, Ongole grade, and their crosses.Â Estrous was synchronized with intravaginal progesterone Cue-Mate before artificial insemination (AI).Â Superovulation was performed with Follicle Stimulating Hormone (FSH) intramuscularly with non-surgical embryo flushing. Â In addition, embryo quality was assessed microscopically according to the International Embryo Transfer Society (IETS) criteria.Â The study was performed in a quasi-experimental design, and data were analyzed with an analysis of variance.Â After superovulation, oocytes/embryos were obtained from all donor breeds.Â Oocyte and embryo production from Ongole grade and Belgian Blue differed at 11.83Â±1.91 and 4.86Â±1.33, respectively, P0.05 (meanÂ±SEM).Â In addition, there are differences in recovery rate (89.63% vs. 75.35%) and fertilization rate (77.35% vs. 68.22%) between Ongole grade and Belgian Blue, respectively (P0.05).Â There is no difference in embryo development quality and proportion of transferable embryos between Ongole grade, Belgian Blue, and their crosses.Â This study concluded that the cross-bred Belgian Blue x Ongole donor had identical oocyte and embryo production, recovery rate, fertilization rate, and degenerative embryos compared to its purebred.

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Section: Introductionmentioning

confidence: 91%

Section: Embryo Qualitymentioning

confidence: 99%

Embryo Production and Development from Superovulated Donors in Double-Muscled Cattle and Their Crosses

Irma,

Rasad,

Hilmia

et al. 2023

JITV

View full text Add to dashboard Cite

show abstract

“…An asset of MALDI is the ability to couple to other MS detectors, for example, tandem MS (MS/MS) for structural characterization or Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) for high-precision measurements enhancing MALDI capabilities and applications in single-cell lipidomics. However, initial applications of MALDI-MS for lipid-based assays were low in spatial resolution, sampling the entirety of a cell, and often applied to a large cell type such as an oocyte (27). A wide range of lipids including sphingomyelins (SMs), phosphatidylcholines (PCs), and TGs were quantifiable, revealing important insights into membrane lipid compositions under various environmental conditions and at various developmental stages of an embryo.…”

Section: Matrix-assisted Laser Desorption/ionization Mass Spectrometrymentioning

confidence: 99%

Analytical Techniques for Single-Cell Biochemical Assays of Lipids

Yao

Vaithiyanathan

Allbritton

2023

Annu. Rev. Biomed. Eng.

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Lipids are essential cellular components forming membranes, serving as energy reserves, and acting as chemical messengers. Dysfunction in lipid metabolism and signaling is associated with a wide range of diseases including cancer and autoimmunity. Heterogeneity in cell behavior including lipid signaling is increasingly recognized as a driver of disease and drug resistance. This diversity in cellular responses as well as the roles of lipids in health and disease drive the need to quantify lipids within single cells. Single-cell lipid assays are challenging due to the small size of cells (∼1 pL) and the large numbers of lipid species present at concentrations spanning orders of magnitude. A growing number of methodologies enable assay of large numbers of lipid analytes, perform high-resolution spatial measurements, or permit highly sensitive lipid assays in single cells. Covered in this review are mass spectrometry, Raman imaging, and fluorescence-based assays including microscopy and microseparations. Expected final online publication date for the Annual Review of Biomedical Engineering, Volume 25 is June 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos

Camargo

Rodrigues²,

Valente

et al. 2022

Livestock Science

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Characteristic MALDI-MS lipid profiles of Gir, Holstein and crossbred (Gir x Holstein) oocytes recovered by ovum pick-up

Cited by 5 publications

References 66 publications

Embryo Production and Development from Superovulated Donors in Double-Muscled Cattle and Their Crosses

Embryo Production and Development from Superovulated Donors in Double-Muscled Cattle and Their Crosses

Analytical Techniques for Single-Cell Biochemical Assays of Lipids

Evaluation of a serum-free culture medium for the enhanced vitrification cryosurvival of bovine in vitro-derived embryos

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