Characteristic of plasmids of <i>Bacillus pumilus</i> isolated in Belarus

Abstract

In current study plasmids (size from 6.2 to 8.5 kb) copied with the “rolling circle” mechanism (RCR type) of the pC194 family have been identified in environmental bacteria Bacillus pumilus. It is shown that these extrachromosomal elements are widely distributed in B. pumilus bacteria circulating on the territory of Belarus (19 strains from 41 contain RCRplasmids) and they are characterized by genetic polymorphism. The most common extrachromosomal genetic elements (7.7 kb size) are identical to the plasmid pBp15.1S from the entomopathogenic strain B. pumilus 15.1. The remaining 6 type of plasmids differ from each other and from the known extrachromosomal genetic elements of genus Bacillus. In the investigated bacteria no replicons similar to pLS20 were detected.

Characterisation of Pectinolytic Bacillus pumilus and Paenibacillus amyloliticus Strains, New Pathogens of Potato in Tunisia

“…isolated in Tunisia was performed in order to reveal intra-species diversity. Similarly to the current research, each of the five Bacillus pumilus strains studied by Galal et al [9] fermented glucose and mannitol, though they were incapable of gelatin liquefaction, contrarily to our work and the studies of Peng et al [11,13,19]. Moreover, the inability of B. pumilus isolates to produce indole in the herein presented study agreed with that of Bathily et al [10].…”

“…So far, little information has been published on the activity of most significant plant cell wall-degrading enzymes among plant pathogenic strains of Bacillus pumilus [9,10,[12][13][14]17]. Evdokimova et al [19] are the sole reporters of lipase and protease action among all the investigated plant pathogenic B. pumilus strains. Notably, some intraspecies variation was noted in cellulase and pectinase activity among phytopathogenic B. pumilus by Evdokimova et al [19], though the capacity to produce these enzymes dominated among the included isolates.…”

“…Evdokimova et al [19] are the sole reporters of lipase and protease action among all the investigated plant pathogenic B. pumilus strains. Notably, some intraspecies variation was noted in cellulase and pectinase activity among phytopathogenic B. pumilus by Evdokimova et al [19], though the capacity to produce these enzymes dominated among the included isolates. Likewise, the common production of pectinases, cellulases, proteases and amylases was exhibited by Bacillus and Paenibacillus spp.…”

“…In more detail, B. pumilus infections led to the development of soft rot in stored potato tubers in Mali [10], bacterial blotch in peach in Egypt [13], wetwood disorder in young Scots pine trees of Ukraine and Belarus [12], ginger rhizome rot in China [11], leaf blight in mango trees in Egypt [9], bacterial fruit rot in muskmelon in China [14] and Ficus lacor in Pakistan [15], Persian oak decline in Iran [16], trunk bulges in rubber trees in Malaysia [17] and interveinal purple-brown leaf spots in common bean in Spain [18]. Additionally, the screening of Belarusian bacterial collections also revealed the occurrence of B. pumilustriggered infections in Cucumis sativus, Solanum lycopersicum, Linum usitatissimum, Solanum tuberosum and Pinus sylvestris [19]. So far, little attention has been paid to the virulence factors responsible for the pathogenic potential of B. pumilus strains [9,10,[12][13][14]17].…”

Assessing immunogenicity of CRISPR-NCas9 engineered strain against porcine epidemic diarrhea virus

“…The presence of antibiotic genes can be diffused through transposition, transfer, or homologous recombination during operation and use, resulting in the emergence of drug-resistant strains (Whittle et al 2002 ). Replication of the plasmid via the rolling circle mechanism leads to the appearance of an unstable single-chain intermediate in the middle link, leading to deletions in the genetic process (Douglas and Klaenhammer 2011 ; Evdokimova et al 2019 ). Although high copy number plasmids might offer certain advantages for antigen expression, plasmid instability and the antibiotic selective pressure make their application in animals challenging (Song et al 2014b ; Zhao et al 2020 ).…”