Characteristic of pollen tube that grew into self style in pear cultivar and parent assignment for cross-pollination

Wang, Guo‐Ming; Gu, Chao; Qiao, Xin; Zhao, Biying; Ke, Ya-Qi; Guo, Binbin; Hao, Ping-Ping; Qi, Kaijie

doi:10.1016/j.scienta.2016.10.035

Cited by 18 publications

(7 citation statements)

References 22 publications

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“…To clarify whether PbLRX.A2.1 and PbLRX.A2.2 are two alleles of one gene in ‘Jinzhui’ and ‘Yali’, droplet digital (ddPCR) was performed to determine the copy number of PbLRX.A2.1 and PbLRX.A2.2 in the ‘Jinzhui’ and ‘Yali’ genomes, which contain only one copy of S 17 ‐RNase and S 21 ‐RNase (Wang et al., 2017). The results showed that the copy numbers of PbLRX.A2.1 and PbLRX.A2.2 were twice that of S 17 ‐RNase in the ‘Jinzhui’ and ‘Yali’ genomes (Figure 7c), suggesting that PbLRX.A2.1 and PbLRX.A2.2 are homologs but not alleles.…”

Section: Resultsmentioning

confidence: 99%

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Self S‐RNase inhibits ABF–LRX signaling to arrest pollen tube growth to achieve self‐incompatibility in pear

Liu

Zhang

et al. 2023

The Plant Journal

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SUMMARY Gametophytic self‐incompatibility (GSI) has been widely studied in flowering plants, but studies of the mechanisms underlying pollen tube growth arrest by self S‐RNase in GSI species are limited. In the present study, two leucine‐rich repeat extensin genes in pear (Pyrus bretschneideri), PbLRXA2.1 and PbLRXA2.2, were identified based on transcriptome and quantitative real‐time PCR analyses. The expression levels of these two LRX genes were significantly higher in the pollen grains and pollen tubes of the self‐compatible cultivar ‘Jinzhui’ (harboring a spontaneous bud mutation) than in those of the self‐incompatible cultivar ‘Yali’. Both PbLRXA2.1 and PbLRXA2.2 stimulated pollen tube growth and attenuated the inhibitory effects of self S‐RNase on pollen tube growth by stabilizing the actin cytoskeleton and enhancing cell wall integrity. These results indicate that abnormal expression of PbLRXA2.1 and PbLRXA2.2 is involved in the loss of self‐incompatibility in ‘Jinzhui’. The PbLRXA2.1 and PbLRXA2.2 promoters were directly bound by the ABRE‐binding factor PbABF.D.2. Knockdown of PbABF.D.2 decreased PbLRXA2.1 and PbLRXA2.2 expression and inhibited pollen tube growth. Notably, the expression of PbLRXA2.1, PbLRXA2.2, and PbABF.D.2 was repressed by self S‐RNase, suggesting that self S‐RNase can arrest pollen tube growth by restricting the PbABF.D.2–PbLRXA2.1/PbLRXA2.2 signal cascade. These results provide novel insight into pollen tube growth arrest by self S‐RNase.

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Section: Resultsmentioning

confidence: 99%

“…The other was fixed in FAA (37% formaldehyde/glacial acetic acid/50% ethanol, 5:5:90) and stored at 4°C until use. Pollen tube growth in the styles of ‘Jinzhui’ was determined following a previously described method (Wang et al., 2017).…”

Section: Methodsmentioning

confidence: 99%

Self S‐RNase inhibits ABF–LRX signaling to arrest pollen tube growth to achieve self‐incompatibility in pear

Liu

Zhang

et al. 2023

The Plant Journal

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“…Since the pollen tube can grow to approximately ⅓ of the full length of the style (Wang et al , 2017), which is required at 8 h after self-pollination, it is considered that self-incompatibility in Rosaceae is a stylar event rather than a stigmatic event. Thus far, studies on Rosaceae self-incompatibility have mainly focused on how the S-RNase of the style recognizes self and nonself pollen after pollination.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq analysis of compatible and incompatible styles of Pyrus species at the beginning of pollination

Guan

Wang

et al. 2018

Preprint

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In Rosaceae, incompatible pollen can penetrate into the style during the gametophytic self-incompatibility response. It is therefore considered a stylar event rather than a stigmatic event. In this study, we explored the differences in gene expression between compatibility and incompatibility in the early stage of pollination. The self-compatible pear variety “Jinzhuili” is a naturally occurring bud mutant from “Yali”, a leading Chinese native cultivar exhibiting typical gametophytic self-incompatibility. We collected the styles of ‘Yali’ and ‘Jinzhuili’ at 0.5 and 2 h after self-pollination and then performed high-throughput sequencing. According to the pathway enrichment analysis of the differentially expressed genes, “plant-pathogen interaction” was the most represented pathway. Quantitative PCR was used to validate these differential genes. The expression levels of genes related to pollen growth and disease inhibition, such as LRR (LEUCINE-RICH REPEAT EXTENSIN), resistance, and defensin, differed significantly between compatible and incompatible pollination. Interestingly, at 0.5 h, most of these genes were upregulated in the compatible pollination system compared with the incompatible pollination system. Calcium ion transport, which requires ATPase, also demonstrated upregulated expression. In summary, the self-incompatibility reaction was initiated when the pollen came into contact with the stigma.

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“…Pear (Pyrus) is identi ed as one of the most important temperate fruit species with high economic value in the world [16]. Multiple internal and external factors participate in the biological processes and characteristics of pear, including pollen growth [17,18], self-incompatibility [19,20], seed germination [21], fruit development and senescence [22,23]. These factors also affect the expressions of related genes.…”

Section: Introductionmentioning

confidence: 99%

Identification and testing of reference genes for qRT-PCR analysis during pear fruit development

et al. 2022

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Background: Quantitative real-time PCR (qRT-PCR) is currently one of the most reliable and improved tools for analyzing gene expression. Various studies have shown that housekeeping genes was varied with cultivars, tissues and treatment. The reliable and stable reference genes were necessarily identi ed and evaluated according to different experimental requirements.Result: In this study, 10 candidate reference genes were initially screened based on the transcriptome sequencing data of four pear fruit development stages of three different pear cultivars, including a candidate housekeeping gene PbrTUB. Furthermore, we ranked the expression stability of 10 candidate reference genes using algorithms GeNorm, NormFinder, BestKeeper and ReFinder. Finally, the result showed that Pbr028511, Pbr038418 and Pbr041114 were the most stable reference gene in Cuiguan, Housui and Xueqing fruit, respectively. Concludion: Thee results provide a valuable resource that serve as signi cant reference for gene function explorations and molecular mechanism studies in fruit development and ripening of different pear cultivars.

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Characteristic of pollen tube that grew into self style in pear cultivar and parent assignment for cross-pollination

Cited by 18 publications

References 22 publications

Self S‐RNase inhibits ABF–LRX signaling to arrest pollen tube growth to achieve self‐incompatibility in pear

Self S‐RNase inhibits ABF–LRX signaling to arrest pollen tube growth to achieve self‐incompatibility in pear

RNA-Seq analysis of compatible and incompatible styles of Pyrus species at the beginning of pollination

Identification and testing of reference genes for qRT-PCR analysis during pear fruit development

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