Characteristics and analysis of normal and leukemic stem cells: current concepts and future directions

Brendel, Cornelia; Neubauer, Andreas

doi:10.1038/sj.leu.2401907

Cited by 35 publications

(21 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Up till now, the reactivity for a high number of proteins has been studied along the maturation of different BM hematopoietic cell lineages (7,12,(17)(18)(19). Despite this, the information currently …”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of the expression of glycosylphosphatidylinositol‐anchored proteins during the maturation of different hematopoietic cell compartments of normal bone marrow

Hernández-Campo

Almeida

Matarraz

et al. 2006

Cytometry Part B Clinical

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of the expression of glycosylphosphatidylinositol‐anchored proteins during the maturation of different hematopoietic cell compartments of normal bone marrow

Hernández-Campo

Almeida

Matarraz

et al. 2006

Cytometry Part B Clinical

View full text Add to dashboard Cite

show abstract

“…Combined analyses of stem cell phenotypic antigen expression, genetic lesions and biological features provide evidence that major differences exist between normal and malignant progenitors. 26 Furthermore, GM-CSF and IL-4 combination did not prove in vitro to induce DC differentiation from normal stem cells. Finally, differentiation of efficient DC from normal stem cells requires a longer period of culture.…”

Section: Figurementioning

confidence: 96%

Identification of precursors of leukemic dendritic cells differentiated from patients with acute myeloid leukemia

et al. 2002

View full text Add to dashboard Cite

Dendritic cells (DC) can facilitate immune responses that might help in the induction of effective antitumor T cell responses. We reported previously that leukemic blasts from selected patients with acute myeloid leukemia (AML) were able to differentiate in vitro into cells with mature DC features. However, despite the use of a wide variety of cytokine combinations, leukemic DC could not be obtained from all AML patients. In this study, we investigated in a wide range of AML patients (n = 30), the nature and functional characteristics of the blast compartment that can be induced to acquire DC features in vitro. Our results demonstrate that leukemic DC generated in the presence of GM-CSF, IL-4 and matured with CD40L, are composed of two major subsets: DC derived from CD14+ leukemic cells and leukemic DC derived from in vivo expanded circulating blood myeloid DC (MDC). Leukemic DC of both subsets exhibited DC morphology, had a phenotype of mature DC, and could induce a potent proliferative response of naive CD4 + T cells. Moreover, both subsets produced large amounts of IL-12p70 and leukemic CD14 + -derived DC could induce a potent Th1 response. These results can be considered as a prerequisite before the design of vaccine immunotherapy protocols for the adjuvant treatment of AML patients.

show abstract

“…1,2 Consequently, as is observed for normal hematopoiesis, LSCs lie at the root of clonal growth and are therefore thought to be responsible for perpetuation of the leukemic population. While the general concept of LSCs is well-established, [3][4][5][6][7][8][9] a formal description of stem cells in AML has only come about as the result of relatively recent studies. Primarily through the efforts of John Dick and colleagues, a specific initial phenotype (CD34 + /CD38 − ) was assigned to the AML stem cell, and the characteristics of this population were defined by transplantation studies using the xenogeneic NOD/SCID mouse model system.…”

Section: Introductionmentioning

confidence: 99%

Unique molecular and cellular features of acute myelogenous leukemia stem cells

Jordan

2002

Leukemia

111

View full text Add to dashboard Cite

It is well known in the field of acute myelogenous leukemia (AML) that many different translocations and genetic aberrancies are found with the various forms of the disease. Indeed, specific translocations are often associated with disease subtypes that manifest themselves through the accumulation of immature myeloid cells at varying stages of differentiation. Moreover, the differentiation state of myeloid blast populations has been utilized as a means of categorizing different AML subtypes (French, American, British, or FAB classification system). Thus, the notion that AML is a family of related but distinct diseases is a common view. Interestingly, however, studies in recent years that have formalized the concept of a leukemic stem cell (LSC) have also begun to define shared developmental, cellular and molecular features amongst the malignant stem cells that give rise to different AML subtypes. Moreover, some of these conserved features appear to be unique to the leukemia stem/progenitor cell population, and are not found in normal hematopoietic stem cells (HSCs). This article will summarize data emerging from the study of LSCs and suggest how distinct molecular and cellular characteristics of the LSC population may provide new opportunities for AML therapy.

show abstract

Characteristics and analysis of normal and leukemic stem cells: current concepts and future directions

Cited by 35 publications

References 77 publications

Quantitative analysis of the expression of glycosylphosphatidylinositol‐anchored proteins during the maturation of different hematopoietic cell compartments of normal bone marrow

Quantitative analysis of the expression of glycosylphosphatidylinositol‐anchored proteins during the maturation of different hematopoietic cell compartments of normal bone marrow

Identification of precursors of leukemic dendritic cells differentiated from patients with acute myeloid leukemia

Unique molecular and cellular features of acute myelogenous leukemia stem cells

Contact Info

Product

Resources

About