1992
DOI: 10.1128/iai.60.9.3673-3681.1992
|View full text |Cite
|
Sign up to set email alerts
|

Characteristics and cariogenicity of a fructanase-defective Streptococcus mutants strain

Abstract: Polymers of D-fructose produced by a variety of oral bacteria are believed to function as extracellular carbohydrate reserves. Degradation of these polysaccharides in plaque following exhaustion of dietary carbohydrates is thought to contribute to the extent and duration of the acid challenge to the tooth surface and thus to the initiation and progression of dental caries. Streptococcus mutans produces a fructanase, the product of the fruA gene, which is capable of degrading beta(2,6)- and beta(2,1)-linked fru… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
11
1

Year Published

1994
1994
2013
2013

Publication Types

Select...
5
2

Relationship

0
7

Authors

Journals

citations
Cited by 22 publications
(12 citation statements)
references
References 39 publications
0
11
1
Order By: Relevance
“…Several attempts have been made to disclose the involvement of FruB, if any, in fructan metabolism. First, a fruA mutant containing a mini-MudE transposon was previously described (59). It is now known, from the results of Northern .…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Several attempts have been made to disclose the involvement of FruB, if any, in fructan metabolism. First, a fruA mutant containing a mini-MudE transposon was previously described (59). It is now known, from the results of Northern .…”
Section: Resultsmentioning
confidence: 99%
“…Enzyme preparation and assays. Protein preparations from exponentially growing cultures of S. mutans were assayed for fructan hydrolase activity as previously described (11,59). One unit of activity was defined as the amount of enzyme needed to release 1 mol of reducing sugar per h. To prepare samples for CAT assays, cleared lysates were prepared as previously detailed (16) and used directly for determination of CAT activity according to the protocol of Shaw (50).…”
Section: Methodsmentioning
confidence: 99%
“…A separate in vitro study was conducted to determine the relationship between the per cent of red stain per sample to bacterial viability. S. mutans UA159 (Wexler et al 1992, Quivey et al 1995, Burne et al 1996 biofilms were grown aerobically in Jordan's medium (Jordan et al 1960) containing 3.5% sucrose in 96-well microtiter plates. The plates were incubated at 33aeC for 24 h and the cultured biofilms were then exposed to either the essential oil mouthrinse or sterile saline (negative control) for 30, 60, 90 or 120 s, after which the test solution was removed and replaced with 250 ml of Letheen neutralizing broth for a minimum of 5 min.…”
Section: Fluorescent Staining and Analysismentioning
confidence: 99%
“…The cell pellets were resuspended in 3.0 ml of 100 mM potassium citrate buffer (KCit), pH 5.5, 1 mM hexanoic acid. Cell-associated protein was prepared as previously described [7,10] using gentle homogenization of the cells in the presence of 1/3 volume of glass beads (average diameter 0.2 ram) with intermittent C02-ethanol cooling. The mixture was then centrifuged at 17000 ×g for 15 245 rain at 4°C and the supernate was dialyzed overnight against KPBH.…”
Section: Enzyme Preparationsmentioning
confidence: 99%