Characteristics and Lytic Activity of Phage-Derived Peptidoglycan Hydrolase, LysSAP8, as a Potent Alternative Biocontrol Agent for Staphylococcus aureus

Yu, Jun-Hyeok; Lim, Joong‐Soo; Chang, Hyun-Joo; Park, Jong‐Hyun

doi:10.4014/jmb.1908.08021

Cited by 19 publications

(13 citation statements)

References 39 publications

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“…The ratio of MBC to MIC is used to characterize the antibacterial activity of any compound. If the MBC/MIC ratio was ≤2, the compounds were termed bactericidal, and they were termed bacteriostatic if the ratio was between 2 and 16 [ 43 , 45 , 58 , 59 , 60 , 61 ]. The results shown in Table 1 suggested that the PIP showed bactericidal and bacteriostatic effects based on the tested bacterial strains.…”

Section: Resultsmentioning

confidence: 99%

Insights into the Antibacterial Activity of Prolactin-Inducible Protein against the Standard and Environmental MDR Bacterial Strains

et al. 2022

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Background: Prolactin inducible protein (PIP) is a small secretary glycoprotein present in most biological fluids and contributes to various cellular functions, including cell growth, fertility, antitumor, and antifungal activities. Objectives: The present study evaluated the antibacterial activities of recombinant PIP against multiple broad-spectrum MDR bacterial strains. Methods: The PIP gene was cloned, expressed and prufied using affinity chromatography. Disk diffusion, broth microdilution, and growth kinetic assays were used to determine the antibacterial activities of PIP. Results: Disk diffusion assay showed that PIP has a minimum and maximum zone of inhibition against E. coli and P. aeruginosa, respectively, compared to the reference drug, ampicillin. Furthermore, growth kinetics studies also suggested that PIP significantly inhibited the growth of E. coli and P. aeruginosa. The minimum inhibitory concentration of PIP was 32 µg/mL for E. coli (443), a standard bacterial strain, and 64 µg/mL for Bacillus sp. (LG1), an environmental multidrug-resistant (MDR) strain. The synergistic studies of PIP with ampicillin showed better efficacies towards selected bacterial strains having MDR properties. Conclusion: Our findings suggest that PIP has a broad range of antibacterial activities with important implications in alleviating MDR problems.

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Section: Resultsmentioning

confidence: 99%

Insights into the Antibacterial Activity of Prolactin-Inducible Protein against the Standard and Environmental MDR Bacterial Strains

et al. 2022

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“…This modularity coupled to synthetic biology tools makes designing chimeric lysins possible by interchanging CDs and CBDs from different origins possible. Chimeric lysins improve the potential for lysins to target pathogens such as Staphylococcus aureus (Bae et al., 2019; Haddad‐Kashani et al., 2018; Idelevich et al., 2011; Mao et al., 2013) and to expand the natural bactericidal spectrum (Yu et al., 2019).…”

Section: Rationale For Phages and Phage Lysinsmentioning

confidence: 99%

Phages and their lysins: Toolkits in the battle against foodborne pathogens in the postantibiotic era

Huang

Zhang

Tong

et al. 2021

Comp Rev Food Sci Food Safe

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Worldwide, foods waste caused by putrefactive organisms and diseases caused by foodborne pathogens persist as public health problems even with a plethora of modern antimicrobials. Our over reliance on antimicrobials use in agriculture, medicine, and other fields will lead to a postantibiotic era where bacterial genotypic resistance, phenotypic adaptation, and other bacterial evolutionary strategies cause antimicrobial resistance (AMR). This AMR is evidenced by the emergence of multiple drug‐resistant (MDR) bacteria and pan‐resistant (PDR) bacteria, which produces cross‐contamination in multiple fields and poses a more serious threat to food safety. A “red queen premise” surmises that the coevolution of phages and bacteria results in an evolutionary arms race that compels phages to adapt and survive bacterial antiphage strategies. Phages and their lysins are therefore useful toolkits in the design of novel antimicrobials in food protection and foodborne pathogens control, and the modality of using phages as a targeted vector against foodborne pathogens is gaining momentum based on many encouraging research outcomes. In this review, we discuss the rationale of using phages and their lysins as weapons against spoilage organisms and foodborne pathogens, and outline the targeted conquest or dodge mechanism of phages and the development of novel phage prospects. We also highlight the implementation of phages and their lysins to control foodborne pathogens in a farm–table–hospital domain in the postantibiotic era.

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“…Expression of the endolysin was induced by adding 0.1 mM IPTG and incubating for 16 h at 18 °C. The KF2_Lys was then purified using Ni-NTA affinity chromatography under native conditions and stored at − 20 °C in storage buffer containing 50 mM Tris-HCl (pH 8.0), 200 mM KCl, 0.1 mM EDTA, 1 mM DTT, and 50% glycerol [11,12].…”

Section: Purification Of Recombinant Vb_vpap_kf2 Endolysinmentioning

confidence: 99%

Characterization of a novel endolysin from bacteriophage infecting Vibrio parahaemolyticus, vB_VpaP_KF2

et al. 2020

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The antimicrobial resistance of food-borne pathogenic bacteria, including Vibrio parahaemolyticus, has been reported globally, warranting the need to identify promising alternative antibiotics such as endolysins that originate from bacteriophages. In our previous study, we characterized a bacteriophage infecting V. parahaemolyticus, vB_VpaP_KF2, at the molecular level. In this study, an open reading frame encoding putative endolysin was cloned from the complete genome data and expressed in the Escherichia coli expression system. The recombinant endolysin, vB_VpaP_KF2_Lys, exhibited a novel lytic property against Gram-negative bacteria regardless of pretreatment with an outer-membrane permeabilizer. It was also stable over a wide range of temperatures, pH, and NaCl concentrations, and its hydrolytic spectrum was broader than that of the parent bacteriophage. From the results, vB_VpaP_KF2_Lys could be used as a biocontrol agent against food-borne pathogens in the field of food safety.

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Characteristics and Lytic Activity of Phage-Derived Peptidoglycan Hydrolase, LysSAP8, as a Potent Alternative Biocontrol Agent for Staphylococcus aureus

Cited by 19 publications

References 39 publications

Insights into the Antibacterial Activity of Prolactin-Inducible Protein against the Standard and Environmental MDR Bacterial Strains

Insights into the Antibacterial Activity of Prolactin-Inducible Protein against the Standard and Environmental MDR Bacterial Strains

Phages and their lysins: Toolkits in the battle against foodborne pathogens in the postantibiotic era

Characterization of a novel endolysin from bacteriophage infecting Vibrio parahaemolyticus, vB_VpaP_KF2

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