Characteristics and Properties of Gelatin from Seabass (Lates calcarifer) Swim Bladder : Impact of Extraction Temperatures

Abstract

This study aimed to investigate the impact of various extracting temperatures on yield and properties of gelatin from swim bladder of seabass (Latescalcarifer), a byproduct from processing. Methods Gelatin from seabass swim bladderwas extracted at different temperatures (45, 55, 65 and 75 °C). The gelatins obtained using various extraction temperatures were characterized. Results The yield and recovery of gelatin from swim bladder(44.83-71.95% and 49.08-74.83%, based on dry weight) increased with increasing extraction temperatures.All gelatins contained α-chainsas the predominant components, followed by β-chain. Gelatin from seabass swim bladder showed a high imino acid content (195 residues/1000 residues). FTIR and CD spectra revealed the loss of triple helix during heating via breaking down hydrogen bonds between α-chains. Gel strength generally increased as the extraction temperature increased up to 65 °C (P<0.05). Gelatin extracted at 65 °C for 6 h showed a higher gel strength, compared to bovine gelatin (P<0.05). Gelling and melting temperatures were 10.4-19.7 and 19.3-28.4 °C, respectively, depending on extraction temperature. Conclusion Properties of gelatin from swim bladder were affected by extraction temperature. Therefore, seabass swim bladder could serve as an alternative collagenous material for gelatin production, when the appropriate extraction condition was implemented.

Physical and sensory properties of gelatin from seabass (Lates calcarifer) as affected by agar and κ‐carrageenan

“…Gelatins were extracted from the skin and the swim bladder of seabass according to the methods of Sinthusamran et al () and Sinthusamran, Benjakul, Hemar, and Kishimura (), respectively. Before gelatin extraction, skin and swim bladder were soaked in 0.1 M NaOH with a sample/solution ratio of 1:10 (w/v) in order to remove non‐collagenous proteins.…”

A novel extraction approach and unique physicochemical properties of gelatin from the swim bladder of sturgeon

“…1, the yield of SG was increased with temperature, time and L / S in a certain range. At 50 °C, 30 min and 10 mL g −1 , it reached to 94.15%, which was much higher than the reported fish skin (11.6–66.4%), bone (2.9–68.8%) and swim bladder (13.5–72.0%) 2–5,14,21,22 . Meanwhile, the AMW of SG was decreased with temperature, time and L / S .…”

“…Recently, fish‐derived gelatin has been attracting more and more attention due to its high safety, less restrictions and abundant resources. It has been extracted from the swim bladders of rohu, seabass, yellowfin tuna, catla, etc 2–5 . But they generally showed low yield, high price, variable quality and inferior properties 6 …”

Effect of preparation method on physicochemical, scavenging, and proliferative properties of gelatin from Yak skin

“…The microstructure of the samples was observed by scanning electron microscopy (SEM) using the method reported by Sinthusamran, Benjakul, Hemar, and Kishimura (2018) and Jridi et al. (2015).…”

“…The microstructure of the samples was observed by scanning electron microscopy (SEM) using the method reported by Sinthusamran, Benjakul, Hemar, and Kishimura (2018) and Jridi et al (2015). The sample was prepared to obtain a thickness of 2-3 mm, fixed on a stub, sputter coated with gold (Hitachi Ion Sputter, MC1000, Japan), and then, observed with an SEM (SU8010, Japan).…”