Characteristics and release profiles of MPEG-PCL-MPEG microspheres containing immunoglobulin G

Erdemli, Özge; Usanmaz, Ali; Keskin, Dilek; Tezcaner, Ayşen

doi:10.1016/j.colsurfb.2014.01.037

Cited by 37 publications

(33 citation statements)

References 58 publications

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“…In all groups, nonirradiated microspheres showed similar shape and surface properties with the γ-irradiated microspheres (data not shown). As observed similarly in our previous study [30], γ-irradiation did not cause any change in the surface properties of PCL microspheres.…”

Section: Resultssupporting

confidence: 79%

“…The encapsulation efficiency of microspheres formulated with different excipients was determined by using hydrolysis of the microspheres with a strong base and the extraction of the protein with SDS as described in detail in our previous study [30]. The amount of IgG encapsulated in microspheres was evaluated by using a micro bicinchoninic acid (μBCA) assay and the absorbance was measured at 562 nm with a microplate reader (μQuant, Biotek, USA) (n = 6).…”

Section: Protein Loading and Encapsulation Efficiencymentioning

confidence: 99%

“…IgG and different excipient loaded PCL microspheres were prepared by the double emulsion-solvent evaporation method used in our previous studies [30,31]. Briefly, 100 μL of the internal aqueous solutions (phosphate buffered saline (PBS), 10 mM sodium phosphate, 145 mM NaCl, pH 7.2) containing 10 mg/mL IgG and different excipients such as PVA (Group P), glucose (Group G), starch (Group S) and heparin (Group H) with known concentrations (Table 1) were added to 2 mL of PCL (5%, w/v) solution in DCM.…”

Section: Preparation Of Immunoglobulin G Loaded Pcl Microspheresmentioning

confidence: 99%

“…Microspheres were sterilized by exposure to γ-irradiation (25 kGy) from 60 Co γ-source (Gamma-cell 220, MDS Nordion, Canada) at ambient temperature and fixed dose rate (1.74 kGy/h) in the Turkish Atomic Energy Authority [18,30,31]. …”

Section: Preparation Of Immunoglobulin G Loaded Pcl Microspheresmentioning

confidence: 99%

See 3 more Smart Citations

Influence of excipients on characteristics and release profiles of poly(ε-caprolactone) microspheres containing immunoglobulin G

Erdemli

Keskin

Tezcaner

2015

Materials Science and Engineering: C

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Section: Resultssupporting

confidence: 79%

Section: Protein Loading and Encapsulation Efficiencymentioning

confidence: 99%

Section: Preparation Of Immunoglobulin G Loaded Pcl Microspheresmentioning

confidence: 99%

Section: Preparation Of Immunoglobulin G Loaded Pcl Microspheresmentioning

confidence: 99%

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Influence of excipients on characteristics and release profiles of poly(ε-caprolactone) microspheres containing immunoglobulin G

Erdemli

Keskin

Tezcaner

2015

Materials Science and Engineering: C

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“…Interestingly, the proposed method for development of NIS-NP herein described showed better results than other published studies. This indicates that the methodology developed here may be considered appropriate for the preparation of peptide-loaded NP with great scale up potential, since it is possible to increase the volume of reaction medium 20-fold with maintaining of the characteristics shown in the experimental results (27,28).…”

Section: Resultsmentioning

confidence: 94%

Development and Characterization of Nisin Nanoparticles as Potential Alternative for the Recurrent Vaginal Candidiasis Treatment

et al. 2016

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Abstract. The aim of this work was the development and characterization of nisin-loaded nanoparticles and the evaluation of its potential antifungal activity. Candidiasis is a fungal infection caused by Candida sp. considered as one of the major public health problem currently. The discovery of antifungal agents that present a reduced or null resistance of Candida sp. and the development of more efficient drug release mechanisms are necessary for the improvement of candidiasis treatment. Nisin, a bacteriocin commercially available for more than 50 years, exhibits antibacterial action in food products with potential antifungal activity. Among several alternatives used to modulate antifungal activity of bacteriocins, polymeric nanoparticles have received great attention due to an effective drug release control and reduction of therapeutic dose, besides the minimization of adverse effects by the preferential accumulation in specific tissues. The nisin nanoparticles were prepared by double emulsification and solvent evaporation methods. Nanoparticles were characterized by dynamic light scattering, zeta potential, Fourier transform infrared, X-ray diffraction, differential scanning calorimetry, and scanning electron microscopy. Antifungal activity was accessed by pour plate method and cell counting using Candida albicans strains. The in vitro release profile and in vitro permeation studies were performed using dialysis bag method and pig vaginal mucosa in Franz diffusion cell, respectively. The results revealed nisin nanoparticles (300 nm) with spherical shape and high loading efficiency (93.88 ± 3.26%). In vitro test results suggest a promising application of these nanosystems as a prophylactic agent in recurrent vulvovaginal candidiasis and other gynecological diseases.

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Synthesis, characterization, and cytotoxicity of star‐shaped polyester‐based elastomers as controlled release systems for proteins

Guo

Zhang

Pei

et al. 2016

J of Applied Polymer Sci

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With the growing number of therapeutic proteins on the market, effective delivery systems are receiving particular attention. In this study, biodegradable elastomers, intended for protein drug delivery and based on methacrylic tripoly(ε‐caprolactone‐co‐d,l‐lactide) cyclic ester with different ratios of ɛ‐caprolactone to d,l‐lactide and methacrylic bipoly[ɛ‐caprolactone‐b‐poly(ethylene glycol)‐b‐ɛ‐caprolactone], were synthesized and characterized. The degradation behavior, bovine serum albumin (BSA)‐releasing kinetics, and cytotoxicity of the elastomers in vitro were investigated. The elastomers were degraded by the hydrolysis of the ester bond; this resulted in pH changes, which further affected the degradation rate. The BSA‐releasing behavior was strongly dependent on the diffusion mechanism. In the diffusion‐controlled period, nearly sustained and stable BSA release was achieved. Furthermore, the elastomers displayed good biocompatibility, as demonstrated by a 3‐(4,5‐dimethyl thiazol‐2‐yl)−2,5‐diphenyl tetrazolium bromide assay and inflammation–induction experiments, and are considered promising candidates for the controllable delivery of protein drugs. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43393.

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Characteristics and release profiles of MPEG-PCL-MPEG microspheres containing immunoglobulin G

Cited by 37 publications

References 58 publications

Influence of excipients on characteristics and release profiles of poly(ε-caprolactone) microspheres containing immunoglobulin G

Influence of excipients on characteristics and release profiles of poly(ε-caprolactone) microspheres containing immunoglobulin G

Development and Characterization of Nisin Nanoparticles as Potential Alternative for the Recurrent Vaginal Candidiasis Treatment

Synthesis, characterization, and cytotoxicity of star‐shaped polyester‐based elastomers as controlled release systems for proteins

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