Characteristics and Whole-Genome Analysis of Limosilactobacillus fermentum Phage LFP02

Abstract

Limosilactobacillus fermentum is a bacterium widely used in food production, medicine, and industrial fermentation. However, fermentation could fail due to phage contamination. L. fermentum bacteriophage LFP02 can be induced from L. fermentum IMAU 32579 using mitomycin C. To better understand the characteristics of this phage, its physiological and genomic characteristics were evaluated. The results showed that its optimal multiplicity of infection was 0.01, and the burst size was 148.03 ± 2.65 pfu/infective center. Compared to temperature, pH had a more obvious influence on phage viability, although its adsorption capacity was not affected by the divalent cations (Ca2+ and Mg2+) or chloramphenicol. Its genome size was 43,789 bp and the GC content was 46.06%, including 53 functional proteins. Compared to other L. fermentum phages, phage LFP02 had chromosome deletion, insertion, and inversion, which demonstrated that it was a novel phage. This study could expand the knowledge of the biological characteristics of L. fermentum bacteriophages and provide some theoretical basis for bacteriophage prevention during fermentation.

Human Rotavirus Genome Copies and Infectious Units in Water and Wastewater in Egypt

“…Sequence analysis is one of the most important methods which confirm the amplified genome positivity in addition to determination of genotypes [51][52][53][54][55]. Sequence anlaysis of the 155 bp of VP6 region of rotavirus in the 38 positive samples showed successful sequencing in 34 samples which contain higher than 10 2 genome copies/litre, while, failure in sequencing was observed for four samples with 10 1 genome copies/litre.…”