Samples of the bacterial plate found at the dissolved O2 H2S interface of Lake Kaiike were incubated at different levels of light and H2S supply. Two species of sluggishly moving bacteria, a large-celled phototrophic bacterium and a bean-shaped one resembling Macromonas bipunctata , were dominant in the bacterial plate. Without supply of H2S these bacteria were displaced by a small coccoid immotile green alga even at a light intensity as low as 400 lux. The rate of H2S produced by the bacterial plate samples, about 0.6 mg S.1-1.day-1,was insufficient to maintain the dense bacterial populations. Two bacteria were considered to be much affected by H2S supply from the underlying layer, although they have different H2S concentrations for their optimal growth.