Characteristics of a Pseudomonas sp. from an Epizootic of Pond-Cultured Eels (Anguilla japonica)

Wakabayashi, Hisatsugu; Egusa, Syuzo

doi:10.2331/suisan.38.577

Cited by 104 publications

(58 citation statements)

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“…The microorganism was first described as the causal agent of red spot disease of Japanese eel (Anguilla japonica) cultured in Japan (Wakabayashi and Egusa, 1972). Subsequentely, the pathogen has been isolated in different countries from a variety of cultured and wild fish species like European eel (Anguilla anguilla), black sea bream (Acanthopagrus schlegeli), ayu (Plecoglossus altivelis), Atlantic salmon (Salmo salar), sea trout (S. trutta), rainbow trout (Onchorhynchus mykiss), whitefish (Coregonus sp.…”

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Development and Validation of a PCR-based Protocol for the Detection of Pseudomonas anguilliseptica

et al. 2004

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ABSTRACT--A PCR-based protocol for the detection of the emerging fish pathogen Pseudomonas anguilliseptica was developed, using the primer set Psan-F/Psan-R designed on the basis of the 16S rRNA sequence.This PCR protocol was optimized and validated by testing 50 target and 38 non-target pure cultures. The method showed a 100% specificity and the detection limit obtained was of 0.7 pgDNA/PCR tube, which equates to 20 or lower bacterial cells. A similar level of sensi tivity was observed when the PCR protocol was applied to fish tissues seeded with bacteria. In addition, the developed PCR assay was also capable of direct detection of P. anguilliseptica from tissues obtained from experimentally infected fish and fish obtained from natural outbreaks in gilthead seabream (Sparus aurata) and turbot (Scophthalmus maximus) farms. This PCR protocol can be a useful tool for a rapid, specific and sensitive diagnosis of fish pseudomonadiasis caused by P. anguilliseptica.

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Development and Validation of a PCR-based Protocol for the Detection of Pseudomonas anguilliseptica

et al. 2004

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“…rieux) (Wakabayashi et al, 1996). Some Pseudomonas species have been reported to be causative agents of fish disease : Pseudomonas anguilliseptica was described as a pathogen in eel (Anguilla japonica) (Wakabayashi & Egusa, 1972) and ayu (Nakai et al, 1985), Pseudomonas fluorescens in carp (Shiose et al, 1974), sea bream (Evynnis japonica) (Kusuda et al, 1974) and tilapia (Sarotherodon niloticus) (Miyashita, 1984), Pseudomonas chlororaphis in amago trout (Onchorhynchus rhodurus) (Hatai et al, 1975) and Pseudomonas putida in yellowtail (Seriola quinqueradiata) (Kusuda & Toyoshima, 1976).…”

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Pseudomonas plecoglossicida sp. nov., the causative agent of bacterial haemorrhagic ascites of ayu, Plecoglossus altivelis.

Nishimori¹,

Kita-Tsukamoto²,

Wakabayashi³

2000

International Journal of Systematic and Evolutionary Microbiology

207

100

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“…fluorescens was originally described as the causative agent of bacterial hemorrhagic septicemia disease of pond-cultured fish [14,15]. It is considered as a primary pathogen of freshwater fish and opportunistic pathogen for different fish species cultured in marine and brackish waters worldwide [16,17].…”

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confidence: 99%

Investigation on the Bacterial Haemorrhagic Septicemia Disease of Cyprinus carpio and Channa striatus

Parvez¹,

Msa²

2014

Poult Fish Wildl Sci

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Bacterial Hemorrhagic Septicemia (BHS) was found affecting Cyprinus carpio and Channa striatus. Bacteriological examination and artificial infection trials were carried out on these fishes. Hematological examination showed substantial decline in the number of erythrocytes and lymphocytes, increase in the number of granulocytes and significant loss in the amount of hemoglobin. Histopathological examination suggested that the affected part of the skin had got completely destroyed. The peripheral area of the liver showed loosening of tissue and cell distension. There was complete shrinkage in all the components of kidney and glomeruli appeared smaller in size than healthy ones. Artificially infected specimens of C. carpio and C. striatus were treated with 20 ppm and 30 ppm chloramphenicol solution and oxytetracycline @ 50 and 60 mg/kg of feed for 20 and 30 days respectively. The results of this work indicated that Aeromonas hydrophila and Pseudomonas fluorescens were the etiological agents for this disease. Investigation on the Bacterial Results and DiscussionBacterial hemorrhagic septicemia was found to affect C. carpio and C. striatus. The diseased specimens ranged between 16 -20cm in total length and 550-625g in weight in case of C. carpio and 13 -16cm in total length and 350-425g in case of weight in C. striatus. As observed in the present case, Kabata (1985) has also reported the occurrence of BHS in C. carpio in Indonesia and West Java. Kumar et al. [24] and Kumar and Dey [25] have reported that all the Asiatic major carps are prone to such infection, but silver carp and catla have been found to be relatively more susceptible.The diseased fish displayed abnormal behavior, swam slowly, remained lethargic, preferred to stay at the bottom of aquarium and refused to feed. They exhibited the symptoms such as the swollen vent filled with reddish fluid, descaling, swelling and softening of the spleen and kidney and the presence of thick yellowish fluid in the intestine. Skin lesions, with hemorrhages, reaching deep into the muscles, appeared on the general surface of the body (Figure 1). Similar symptoms of BHS have also been reported by [2,3,8,20,26,27].The bacteriological examination of the diseased fishes indicated that mainly two pathogenic bacteria, Aeromonas hydrophila and Pseudomonas fluorescens have been associated with this disease. After 24-48 hours of incubation at 28 °C , numerous small colonies appeared which were subsequently isolated in pure form on TSA. A. hydrophila was gram-negative rod, motile, oxidase-positive, and catalase-positive and glucose fermenting bacteria while Pseudomonas fluorescens was gram-negative rod, motile, oxidase-positive, catalase-positive and glucose oxidizing bacteria. Other biochemical characteristics of these bacterial species are given in Table 1. The characteristics exhibited by these bacteria conform to those as given by [20,22,28].Experimental infection trials were carried out by keeping healthy specimens of C. carpio and C. striatus in water at 28°C inocula...

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Characteristics of a Pseudomonas sp. from an Epizootic of Pond-Cultured Eels (Anguilla japonica)

Cited by 104 publications

References 0 publications

Development and Validation of a PCR-based Protocol for the Detection of Pseudomonas anguilliseptica

Development and Validation of a PCR-based Protocol for the Detection of Pseudomonas anguilliseptica

Pseudomonas plecoglossicida sp. nov., the causative agent of bacterial haemorrhagic ascites of ayu, Plecoglossus altivelis.

Investigation on the Bacterial Haemorrhagic Septicemia Disease of Cyprinus carpio and Channa striatus

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