Characteristics of an environmental strain, Enterococcus faecalis CECT7121, and its effects as additive on craft dry-fermented sausages

Sparo, Mónica; Nuñez, Guillermo Gabriel; Castro, Marisa; Calcagno, María de Luján; Allende, M.A. García; Ceci, Mónica; Najle, Roberto; Manghi, Marcela Alejandra

doi:10.1016/j.fm.2008.01.008

Cited by 40 publications

(46 citation statements)

References 22 publications

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“…This lack of translocation might be because this strain does not adhere in the large intestine and because the Ef7121 levels in the small intestine enhance an immune response that restricts excessive growth of the strain, thus preventing its translocation. The bacteria that translocate most efficiently, such as Salmonella typhimurium and L. monocytogenes (Duffy 2000), are classified as facultative intracellular pathogens; Ef7121 is not included in this class (Sparo et al 2008). In addition, all mice that received the probiotic remained healthy for the duration of the study.…”

Section: Discussionmentioning

confidence: 99%

“…Ef7121 intestinal colonization was evaluated by recounting the bacteria present in the intestine (CFU/g). The phenotypic characterization of enterococci and enterobacteria was carried out by Gram staining, cultivation in differential media and biochemical tests (Sparo et al 2008). Ef7121 colonies were identified based on their inhibitory activity against Listeria monocytogenes CEB101 ( Sparo et al 2008).…”

Section: In Vivo Assaysmentioning

confidence: 99%

“…The phenotypic characterization of enterococci and enterobacteria was carried out by Gram staining, cultivation in differential media and biochemical tests (Sparo et al 2008). Ef7121 colonies were identified based on their inhibitory activity against Listeria monocytogenes CEB101 ( Sparo et al 2008). The mesenteric lymph nodes and spleens of inoculated mice were dissected and cultured to evaluate any possible bacterial translocation (Basualdo et al 2007).…”

Section: In Vivo Assaysmentioning

confidence: 99%

“…The intestine, liver and lungs were dissected. Ef7121 colonization of the intestine was assessed by CFU counts (CFU/g) (Sparo et al 2008). The quantity of T. canis larvae in the liver and lungs was determined by light microscopy prior to peptic digestion (1% pepsin in pH 1.5 glycine buffer).…”

Section: In Vivo Assaysmentioning

confidence: 99%

“…Hemolysin and gelatinase are virulence factors involved in the translocation of these enterococci. E. faecalis CECT7121 lacks genes that code for hemolysin and gelatinase and is devoid of a capsule (Sparo et al 2008). Electrophoretic analysis of plasmid DNA revealed that E. faecalis CECT7121 contains only one plasmid.…”

mentioning

confidence: 99%

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In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis

Chiodo

Sparo

Pezzani

et al. 2010

Mem. Inst. Oswaldo Cruz

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The aim of the present paper was to evaluate the larvicidal effect of Enterococcus faecalis CECT7121 (Ef7121) on the Toxocara canis cycle both in vitro and in vivo. For the in vitro experiments, T. canis larvae were incubated with the supernatants of Ef7121 (EI) and mutant Ef7121 (EIm), in a pre-culture of Ef7121 (EII) and in a fresh culture with Ef7121 (EIII) and the Ef7121 mutant strain (EIIIm). The viability of the larvae was calculated after a 48 h incubation. A significant reduction of the viability of T. canis larvae was observed in EI, EII and EIII. A decrease of this inhibitory effect was observed in EIm and EIIIm (p = 0.008). In the in vivo experiments, mice were orally inoculated with three doses of Ef7121. To study the probiotic persistence in the intestine, the animals were sacrificed every four days and their intestines were dissected. The initial average bacterial levels were 9.7 x 10(4) for Ef7121 (colony forming units/g). At the end of the assay the levels were 1.46 x 10(4). No bacterial translocation was detected in mesenteric lymphatic nodules and spleen. Ef7121 interference with the biological cycle was evaluated in mice challenged with T. canis. The interference was significant when the mice were challenged with probiotic and T. canis simultaneously (p = 0.001), but it was not significant when the challenge was performed 15 days after administration of the bacterial inoculum (p = 0.06). In conclusion, Ef7121 possessed in vitro and in vivo larvicidal activity

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Section: Discussionmentioning

confidence: 99%

Section: In Vivo Assaysmentioning

confidence: 99%