Characteristics of an unclassified Mycobacterium species isolated from patients with Crohn's disease

Chiodini, Rodrick J.; Kruiningen, Herbert J. Van; Merkal, R. S.; Thayer, Walter R.; Coutu, J A

doi:10.1128/jcm.20.5.966-971.1984

Cited by 269 publications

(110 citation statements)

References 18 publications

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“…54 The disease becomes evident after a latent period of several years. 53 Failure to isolate mycobacteria from humans with Crohn's disease does not disprove mycobacteria as the cause, because the organism is difficult to isolate. Mycobacteria are often not found in humans with disease known to be caused by the organism, and mycobacterial infections in humans are not always serologically detectable.…”

Section: Etiopathogenesis Of Gementioning

confidence: 99%

Chronic Idiopathic Inflammatory Bowel Diseases of the Horse

Schumacher

Edwards

Cohen

2000

Veterinary Internal Medicne

101

180

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A review of reported cases of inflammatory bowel diseases (IBDs) of horses for which no etiology was identified included cases of granulomatous enteritis (GE), multisystemic eosinophilic epitheliotropic disease (MEED), lymphocytic-plasmacytic enterocolitis (LPE), and idiopathic eosinophilic enterocolitis (EC). The terms EC and MEED were both used to describe a disease in horses characterized by infiltration of intestine and extraintestinal tissues with eosinophils. We use EC to describe IBD characterized by only intestinal infiltration by eosinophils. Horses with GE, MEED, or LPE are usually examined because of weight loss and depression, but horses with EC are usually examined because of signs of abdominal pain. Typically, horses with IBD have low concentrations of serumal proteins, especially albumin, and fail to adequately absorb glucose or xylose. Antemortem diagnosis of IBD can only be made by histologic examination of affected intestine. In some cases, antemortem diagnosis is made from histologic examination of rectal mucosa obtained by biopsy. Suspected causes of IBD in the horse include abnormal immune response to bacterial, viral, parasitic, or dietary antigens. Most horses with IBD do not survive, but horses with EC are more likely than those with LPE, MEED, or GE to respond to treatment. Successful treatments of horses with IBD include resection of grossly affected intestine and administration of corticosteroids.Key words: Eosinophilic enterocolitis; Granulomatous enteritis; Lymphocytic-plasmacytic enterocolitis; Multisystemic eosinophilic epitheliotropic disease.C hronic inflammatory bowel diseases (IBDs) are associated with dysfunction of the gastrointestinal tract due to infiltration of the mucosa and submucosa with populations of eosinophils, plasma cells, lymphocytes, basophils, or macrophages. 1,2 Clinical signs of IBD in the horse include weight loss, dependent edema, and lethargy and are usually associated with protein-losing enteropathy and malabsorption of nutrients, including glucose. Affected horses may have diarrhea or signs of abdominal pain. Signalment, clinical signs, clinicopathologic findings, and postmortem lesions may be highly characteristic of some types of IBD in the horse, but, in general, clinical and gross pathologic features of these diseases are similar, and a final morphologic diagnosis is based on histologic examination. When an etiologic diagnosis of IBD has been reported, mycobacterial 3-12 or parasitic infections, 13,14 or toxins 15 have been identified. The purpose of this report is to describe distinguishing features and discuss possible etiologies of the idiopathic IBDs and to discuss treatment of affected horses. Granulomatous Enteritis (GE)This idiopathic condition was 1st reported in 1974 by Cimprich, 7 who noted the histologic similarity between this disease and Crohn's disease in humans. In our review of the reports of idiopathic GE, we found detailed descriptions of 47 affected horses. 6,7,9,[16][17][18][19][20][21][22][23][24][25] Of these, 32 were Standardbr...

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Section: Etiopathogenesis Of Gementioning

confidence: 99%

Chronic Idiopathic Inflammatory Bowel Diseases of the Horse

Schumacher

Edwards

Cohen

2000

Veterinary Internal Medicne

101

180

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“…Conflicting results have been reported concerning M. paratuberculosis (Gaya et al, 2004;Huggett et al, 2004). Some authors showed that these bacteria can be isolated from tissues of patients with CD (Chiodini et al, 1984), but studies using immunohistochemistry detection or polymerase chain reaction techniques disagree over its aetiological role (Sanderson et al, 1992;Dell'Isola et al, 1994;Rowbotham et al, 1995). The identification of Mycobacterium avium ssp.…”

Section: Introductionmentioning

confidence: 99%

Adherent-invasive Escherichia coli isolated from Crohn's disease patients induce granulomas in vitro

et al. 2007

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SummaryAdherent-invasive Escherichia coli (AIEC) have been shown to be highly associated with ileal Crohn's disease (CD). AIEC survive within infected macrophages, residing within the phagolysosomal compartment where they take advantage of the low pH to replicate extensively. We investigated whether, like the tuberculous bacillus which also persists within macrophages, AIEC LF82 induces the formation of granulomas, which are a common histopathological feature of CD. For this purpose, we have taken advantage of an in vitro model of human granulomas that we recently developed, based on blood-derived mononuclear cells. We demonstrated that AIEC LF82 induces aggregation of infected macrophages, fusion of some of them to form multinucleated giant cells and subsequent recruitment of lymphocytes. Light microscopy and scanning electron microscopy analysis of the cell aggregates confirmed their granuloma features. This was further confirmed by histological analysis of granuloma sections. Noteworthy, this phenomenon can be reproduced by soluble protein extracts of AIEC LF82 coated onto beads. Although the cell aggregates not completely mimic natural CD-associated granulomas, they are very similar to early stages of epithelioid granulomas.

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“…Crohn's disease (CD) and ulcerative colitis (UC) is still unknown [1]. However, experimental support for the early hypothesis that bacteria or their products might be involved in disease pathogenesis [2,3] has been presented by a large number of studies, which either searched to define a single pathogenetic bacterium causing the disease [4][5][6] or investigated the possibility that intestinal inflammation results from hyperresponsiveness to ubiquitous bacterial antigens or products of the resident intestinal flora [7,8]. Although there is to date no convincing evidence for the aetiological involvement of any defined microbial species in IBD, this is still an area of active research and, as in a recent report providing immunohistochemical evidence for a role for Listeria species (spp.…”

Section: Introductionmentioning

confidence: 99%

Bacteria‐Specific T‐Cell Clones are Selective in their Reactivity Towards Different Enterobacteria or H. pylori and Increased in Inflammatory Bowel Disease

1996

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In the present study the authors investigated the T-cell response to different enterobacteria or Helicobacter pylori and tested the hypothesis that the frequency of bacteria-specific T cells is increased in the intestine of patients with active inflammatory bowel disease (IBD), i.e. Crohn's disease (CD) and ulcerative colitis (UC). The analysis of a large panel of T-cell clones (Tc) (n = 888) from peripheral blood, non-inflamed and inflamed intestine from IBD patients and control individuals shows that both peripheral blood and intestinal T-cell clones were selectively stimulated by either Salmonella typhimurium, Yersinia enterocolitica 03, Escherichia coli or Helicobacter pylori sonicates, that only < 3% of all bacteria-reactive Tc were crossreactive and that proliferation to bacterial sonicates was inhibited by anti-MHC class II antibody. In addition, bacteria-specific Tc from IBD patients were more frequently isolated from inflamed intestine than from peripheral blood (P = 0.0039) or non-inflamed intestine. These data, from a large number of T-cell clones, are the first systematic analysis describing the response of individual T cells towards different bacterial species (ssp.). They show that T cells with specificity for distinct antigens or superantigens that are characteristic for a defined bacteria ssp. are present in normal, and increased in inflamed, IBD-intestine. These bacteria-specific Tc may play a role in IBD pathogenesis.

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Characteristics of an unclassified Mycobacterium species isolated from patients with Crohn's disease

Cited by 269 publications

References 18 publications

Chronic Idiopathic Inflammatory Bowel Diseases of the Horse

Chronic Idiopathic Inflammatory Bowel Diseases of the Horse

Adherent-invasive Escherichia coli isolated from Crohn's disease patients induce granulomas in vitro

Bacteria‐Specific T‐Cell Clones are Selective in their Reactivity Towards Different Enterobacteria or H. pylori and Increased in Inflammatory Bowel Disease

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