Characteristics of asthmatics with detectable IL-32γ in induced sputum

Kwon, Jae-Woo; Chang, Hun Soo; Heo, Jeong-Seok; Bae, Da-Jeong; Lee, Jong-Uk; Jung, Chang An; Son, Ji-Hye; Park, Jong Suk; Kim, Soo hyun; Min, Kyung-Up; Park, Choon-Sik

doi:10.1016/j.rmed.2017.06.005

Cited by 10 publications

(3 citation statements)

References 37 publications

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“…IL-32 has increasingly been suggested as a key player in the pathophysiology of asthma. In asthma, the airway presence of IL-32γ is negatively correlated with the forced expiratory volume in 1 s (FEV1) and positively correlated with the annual exacerbation rate [ 60 ]. In that study, the authors concluded that the increased IL-32γ level in the induced sputum samples correlated with an increased risk of asthma exacerbation.…”

Section: Introductionmentioning

confidence: 99%

Interleukin-32: its role in asthma and potential as a therapeutic agent

et al. 2018

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Interleukin (IL)-32, also named natural killer cell transcript 4 (NK4), has increasingly been described as an immunoregulator that controls cell differentiation and cell death and is involved in the stimulation of anti−/pro-inflammatory cytokines. Abnormal presence of IL-32 has been repeatedly noticed during the pathogenesis of allergic, infectious, cancerous, and inflammatory diseases. Of particular note was the observation of the anti-inflammatory property of IL-32 in a murine ovalbumin model of allergic asthma. Compared to wild-type mice, IL-32γ transgenic mice show decreased levels of inflammatory cells, recruited eosinophils, and lymphocytes in bronchoalveolar lavage fluid in a mouse model of acute asthma. To date, the molecular mechanism underlying the role of IL-32 in asthma remains to be elucidated. This review aims to summarize recent advances in the pathophysiology of asthma and describe the links to IL-32. The possibilities of using IL-32 as an airway inflammation biomarker and an asthma therapeutic agent are also evaluated.

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Section: Introductionmentioning

confidence: 99%

Interleukin-32: its role in asthma and potential as a therapeutic agent

et al. 2018

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“…As mentioned above, IL-32q has been suggested to play an intracellular modulatory role in breast cancer cells (56,59). On the other hand, a study conducted on asthma patients showed a lower IL-32g compared to healthy controls (37) as well as rIL-32g that showed a negative regulatory effect in an asthma mouse model (14). However, this report did not consider the behavior of the IL-32q isoform.…”

Section: Discussionmentioning

confidence: 81%

Comparison of the Seven Interleukin-32 Isoforms’ Biological Activities: IL-32θ Possesses the Most Dominant Biological Activity

et al. 2022

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Cytokines are significantly associated with the homeostasis of immune responses in health and disease. Interleukin-32 (IL-32) is a cytokine originally discovered in natural killer cell transcript 4. IL-32 with different disorders has been described in terms of pathogenesis and the progression of diseases. Clinical studies have investigated IL-32 under various conditions, such as viral infection, autoimmune diseases, inflammatory diseases, certain types of cancer, vascular disease, and pulmonary diseases. The high expression of IL-32 was identified in different tissues with various diseases and found to have multiple transcripts of up to seven isoforms. However, the purification and biological activities of these isoforms have not been investigated yet. Therefore, in this study, we purified and compared the biological activity of recombinant IL-32 (rIL-32) isoforms. This is the first time for seven rIL-32 isoforms (α, β, δ, γ, ϵ, ζ, and θ) to be cloned and purified using an Escherichia coli expression system. Next, we evaluate the biological activities of these seven rIL-32 isoforms, which were used to treat different types of cells by assessing the levels of inflammatory cytokine production. The results revealed that rIL-32θ possessed the most dominant biological activity in both immune and non-immune cells.

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“…Patients were enrolled from Soonchunhyang University Bucheon Hospital (11). Asthma was diagnosed by physicians based on the Global Initiative for Asthma guidelines (12).…”

Section: Study Participantsmentioning

confidence: 99%