Characteristics of 'Baekjung', a variety adaptable to high temperature in Flammulina velutipes

김은선,; 우성이,; 오민지,; 오연이,; 신평균,; 장갑열,; 공원식,; 이창수,

doi:10.14480/jm.2015.13.3.203

Cited by 2 publications

(3 citation statements)

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“…The edible mushroom Flammulina velutipes belongs to the family Tricholomataceae within Agaricales and grows on old trees or the stumps of various broadleaf trees from late autumn to the following spring. This mushroom is a cold-resistant fungus that occurs even in winter; therefore, it is also called the winter mushroom [ 1 , 2 ]. Wild-type F. velutipes strains vary in color from yellowish-brown to dark brown, whereas artificial cultivars are predominantly white [ 3 ] ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

“…Since the artificial cultivation of F. velutipes began, various white cultivars have been actively developed, but the close genetic relationship between these varieties limits the development of new cultivars [ 3 , 4 ]. To overcome these limitations, new varieties are being actively developed through crossbreeding with various wild strains, including non-white ones [ 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. Recent studies have analyzed nutritional components, such as saccharides, amino acids, and organic acids, as well as the growth characteristics of new varieties, and a comparative analysis of nutrients between white and non-white varieties was also conducted [ 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…To overcome these limitations, new varieties are being actively developed through crossbreeding with various wild strains, including non-white ones [ 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. Recent studies have analyzed nutritional components, such as saccharides, amino acids, and organic acids, as well as the growth characteristics of new varieties, and a comparative analysis of nutrients between white and non-white varieties was also conducted [ 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. Information on the physiological characteristics and differences of these F. velutipes varieties, including the color of the F. velutipes fruiting body, can be used for breeding newly developed cultivars.…”

Section: Introductionmentioning

confidence: 99%

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Phenylalanine Ammonia-Lyase: A Key Gene for Color Discrimination of Edible Mushroom Flammulina velutipes

Park

et al. 2023

JoF

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In nature; Flammulina velutipes, also known as winter mushrooms, vary in the color of their fruiting bodies, from black, yellow, pale yellow, or beige to white. The purpose of this study was to compare the genome sequences of different colored strains of F. velutipes and to identify variations in the genes associated with fruiting body color. Comparative genomics of six F. velutipes strains revealed 70 white-strain-specific variations, including single nucleotide polymorphisms (SNPs) and insertions/deletions (indels), in the genome sequences. Among them, 36 variations were located in the open reading frames, and only one variation was identified as a mutation with a disruptive in-frame deletion (ΔGCGCAC) within the annotated gene phenylalanine ammonia-lyase 1 (Fvpal1). This mutation was found to cause a deletion, without a frameshift, of two amino acids at positions 112 and 113 (arginine and threonine, respectively) in the Fvpal1 gene of the white strain. Specific primers to detect this mutation were designed, and amplification refractory mutation system (ARMS) polymerase chain reaction (PCR) was performed to evaluate whether the mutation is color specific for the F. velutipes fruiting body. PCR analysis of a total of 95 F. velutipes strains revealed that this mutation was present only in white strains. In addition, monospores of the heterozygous mutant were isolated, and whether this mutation was related to the color of the fruiting body was evaluated by a mating assay. In the mating analysis of monospores with mutations in Fvpal1, it was found that this mutation plays an important role in determining the color of the fruiting body. Furthermore, the deletion (Δ112RT113) in Fvpal1 is located between motifs that play a key role in the catalytic function of FvPAL1. These results suggest that this mutation can be used as an effective marker for the color-specific breeding of F. velutipes, a representative edible mushroom.

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Section: Introductionmentioning

confidence: 99%