Characteristics of Cellular Immune Status in the Patients With Chronic Rhinosinusitis With Nasal Polyps

Савлевич, Е. Л.; Хайдуков, С. В.; Курбачева, О М; Бондарева, Г. П.; Shachnev, K. N.; Симбирцев, А. С.

doi:10.15789/1563-0625-2017-6-731-738

Cited by 9 publications

(3 citation statements)

References 8 publications

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“…Чтобы изучить изменение количества субпопуляций лимфоцитов в зависимости от возраста, Оригинальные статьи системного иммунитета как основы оценки состояния иммунной системы онкологических больных. Границы нормальных показателей для лимфоцитов в линейных популяциях (Т-клеток, в том числе CD3 + CD4 + -и CD3 + CD8 + -лимфоцитов, В-и NK-клеток), полученные в нашем исследовании, были сопоставимы с данными исследований как российских авторов, так и зарубежных [4][5][6][7]. Однако такие субпопуляции клеток, как NKT-или CD3 -CD8 + -лимфоциты, отсутствуют в сравнительной характеристике линейных популяций у этих авторов.…”

Section: результатыunclassified

Subpopulation Structure of Peripheral Blood Lymphocytes of Donors

Борунова

Заботина

Шоуа

et al. 2020

Rossijskij bioterapevtičeskij žurnal

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Introduction. Long-term monitoring of immune system parameters in cancer patient in FSBI “N.N. Blokhin Medical Research Center of Oncology” of the Ministry of Health of Russia, as well as the absence of an immunological research algorithm and a spectrum of significant markers, served as the basis for this study.Purpose. To present reference values and determine the normal range for subpopulations of systemic immunity lymphocytes.Materials and methods. The phenotype of peripheral blood lymphocytes was studied in 186 healthy donors (86 men and 100 women), mean age 41,9 ± 12,5 years. To assess the multivariate phenotype by flow cytometry, monoclonal antibodies to CD45, CD3, CD4, CD8, CD16, CD56, CD19, CD25, CD28, CD11b, CD127, HLA-Dr, TCR-γ / δ, Perforin and Granzime B labeled various fluorochromes.Results. A panel of markers of immunocompetent cells of systemic immunity was developed as a basis for assessing the state of the immune system of cancer patients. Reference values and normal boundaries are given for characterizing the linearity of cells with detailing of the phenotypic and functional heterogeneity of the population of CD8+-lymphocytes, activation markers and the pool of naive cells; characteristics of various types of regulatory cells, functional activity of cells of the effector link of immunity. A comparative analysis of immunoregulatory indices was carried out and the vulnerability of the formula CD3+CD4+/CD3+CD8+ to the CD4+/CD8+ index was proved.Conclusion. The spectrum of the proposed indicators is the product of many years of research carried out in the laboratory of clinical immunology of the Federal State Budgetary Institution “N.N. Blokhin National Medical Research Center of Oncology” оf the Ministry of Health of the Russian Federation, in order to search for significant criteria for assessing the state of the immune system in cancer.

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Section: результатыunclassified

Subpopulation Structure of Peripheral Blood Lymphocytes of Donors

Борунова

Заботина

Шоуа

et al. 2020

Rossijskij bioterapevtičeskij žurnal

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“…Полипозный риносинусит следует рассматривать как фактор риска формирования тяжёлой и плохо контролируемой БА. Поскольку воспаление верхних дыхательных путей поддерживает воспаление нижних дыхательных путей и наоборот [8][9][10], такие пациенты чаще развивают обострения БА, поэтому им необходима госпитализация для купирования патологического состояния, а лечение требует более длительного времени [10].…”

Section: Introductionunclassified

Dupilumab: new opportunities for the treatment of asthma and chronic rhinosinusitis with nasal polyps

Dyneva

Есенгельдыевна²,

Aminova

et al. 2021

Russian Journal of Allergy

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Airway inflammation plays a key role in asthma and chronic rhinosinusitis with nasal polyps (CRSwNP). The inflammatory process can vary in intensity thus affecting the clinical picture of the disease and, most importantly, the effectiveness of therapy. Today, there is still a high rate of growth in the incidence of asthma and CRSwNP and dissatisfaction with the effectiveness of existing therapy for severe forms of asthma, especially when asthma is associated with CRSwNP, so the main task is to find new approaches to diagnosis and therapy. The development of biologics is a promising step forward in achieving control of severe and poorly controlled asthma and recurrent CRSwNP that target individual and specific components of inflammation. One of the latest monoclonal antibodies is Dupilumab that has shown significant success in the treatment of asthma and CRSwNP. Dupilumab is a fully human monoclonal antibody directed against the subunit of the Il-4 interleukin receptor (IL-4ra), common to both IL-4 and IL-4/IL-13 receptor complexes. This contributes to the suppression of type 2 cytokine signaling (IL-4 and IL-13), since the IL-4/IL-13/STAT6 signaling pathway plays a crucial role in type 2- inflammation. Currently, Dupilumab is approved for the treatment of severe asthma and CRSwNP, so this article summarizes the main information about Dupilumab and its effectiveness in these diseases, as well as presents the results of clinical observation.

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“…А повышение абсолютного и относительного количества Т-регуляторных клеток (CD4 + CD25 bright CD127 low to neg ), которые своим супрессивным действием на эффекторные клетки адаптивного иммунного ответа способствуют неполной элиминации инфекционных агентов, усугубляет их персистенцию на слизистой носа и околоносовых пазух (ОНП) и содействует неполноценному течению воспаления при этой патологии [6]. Проведенный нами сравнительный анализ показателей клеточного системного иммунитета между группами пациентов с разными фенотипами ПРС (с сопутствующей БА, атопией и без коморбидной патологии) не выявил статистически значимой разницы между группами [7]. Также мы не получили достоверных отличий при сравнении групп в зависимости от степени отклонения показателей значений натуральных киллеров (CD3 -CD16 + CD56 + ), активированных натуральных киллеров (CD3 -CD8 + ) и Т-регуляторных клеток (CD4 + CD25 CD127) от нормы [5].…”

Section: Introductionunclassified

Characteristics of Cellular Compartment Changes of Immune System in the Patients With Chronic Polypous Rhinosinusitis Depend on Efficiency of Drug Therapy

2019

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Despite numerous attempts to control the course of chronic rhinosinusitis with nasal polyps (CRSwNP) by means of pharmacological treatment and new surgical approaches, the majority of patients experience lifelong persistence of this disorder, at recurrence rates of 50-60% within 18 months after surgical treatment. Since CRSwNP is a chronic persistent inflammatory process, it affects the entire body condition, including the state of systemic immune response. An elevation of NK (CD3-CD16+CD56+), activated NK (CD8+CD3-), NKT cells (CD16+CD56+CD3+), Treg (CD4+CD25brightCD127low to neg) cells and activated T-lymphocytes (CD3+CD25+) was revealed elsewhere among all the patients with CRSwNP, using a flow ytometry method. There was no difference between various disease phenotypes. We analyzed the status of cellular component of systemic immunity, dependent on clinical course of the disease and efficiency of the administered therapy of CRSwNP. The patients were divided into three subgroups. The follow-up period was 1 year. The first group comprised the patients who showed positive dynamics after conservative therapy, resulting into regression of nasal polyps and their grade than a year ago. The second group included the patients in whom the size of polyps remained the same. The third group included the patients with higher incidence of nasal polyps than a year ago.We have shown a decrease of Treg, NKT cells, NK and activated NK, cytotoxic T-lymphocytes (CD3+CD8+), activated T-cell numbers in clinical group 3 with aggressive growth of polyps and low effect of standard therapy, which may cause deterioration of the immune system cellular populations, accompanied by presence of persistent productive inflammatory process of nasal cavity and paranasal sinuses. In the second group, a significant elevation of total lymphocyte number, total and activated T cells, T helpers (CD3+CD4+), cytotoxic T lymphocytes, NK and NKT cells was shown. Meanwhile, a decrease in absolute number of activated NK was observed despite the NK growth. Therefore, we can assume that the mechanism of their activation was disturbed and compensated by production of NKT cells and cytotoxic T lymphocytes. Moreover, we have shown in this group that the absolute number of Treg cells is increased; and these cells had a suppressive influence on effector cells of adaptive immune response, thus inducing incomplete elimination of infectious agents, which contribute to permanent incomplete course of inflammatory process. Chronic inflammatory process in CRSwNP affects systemic cellular immunity depending on the morbidity characteristics in the course of pathological process. The maximal intensity of systemic cellular immunity is observed in the group of patients that require permanent basic drug therapy. In case of aggressive CRSwNP and failure of standard drug therapy, we observed a decrease in absolute numbers of effector cells, along with decreased Treg lymphocyte numbers which may explain inefficient immune regulation of inflammatory process and medical interventions in this group of patients.

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Characteristics of Cellular Immune Status in the Patients With Chronic Rhinosinusitis With Nasal Polyps

Cited by 9 publications

References 8 publications

Subpopulation Structure of Peripheral Blood Lymphocytes of Donors

Subpopulation Structure of Peripheral Blood Lymphocytes of Donors

Dupilumab: new opportunities for the treatment of asthma and chronic rhinosinusitis with nasal polyps

Characteristics of Cellular Compartment Changes of Immune System in the Patients With Chronic Polypous Rhinosinusitis Depend on Efficiency of Drug Therapy

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