Characteristics of cyanobacterium Pseudanabaena galeata CCNP1313 from the Baltic Sea

Cegłowska, Marta; Toruńska-Sitarz, Anna; Stoń-Egiert, Joanna; Mazur‐Marzec, Hanna; Kosakowska, Alicja

doi:10.1016/j.algal.2020.101861

Cited by 11 publications

(9 citation statements)

References 67 publications

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“…Fluorescence spectroscopy is a very sensitive technique and is less laborious than HPLC based methods, which require chromatographic separation, that are commonly used to quantify other cyanobacteria marker pigments like chlorophyll a or echinenone (Schalles and Yacobi, 2000;Poza-Carrión et al, 2001;Schlüter et al, 2004;Seppälä et al, 2007;Arróniz-Crespo et al, 2014). Phycocyanin measurements have been extensively used to quantify cyanobacteria in pure cultures (Herrera et al, 1989;Lee et al, 2017;Basheva et al, 2018;Piron et al, 2019) and water samples (Izydorczyk et al, 2005;Cotterill et al, 2019;Cegłowska et al, 2020) but, to our knowledge, it has never been applied to moss samples. We proposed that phycocyanin quantification could be a reliable proxy to estimate moss-associated cyanobacteria quantity.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Moss-Associated Cyanobacteria Using Phycocyanin Pigment Extraction

2021

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In the boreal forest, cyanobacteria can establish associations with feather moss and realize the biological nitrogen fixation (BNF) reaction, consisting in the reduction of atmospheric dinitrogen into bioavailable ammonium. In this ecosystem, moss-associated cyanobacteria are the main contributors to BNF by contributing up to 50% of new N input. Current environmental changes driven by anthropogenic activities will likely affect cyanobacteria activity (i.e., BNF) and populations inhabiting mosses, leading to potential important consequences for the boreal forest. Several methods are available to efficiently measure BNF activity, but quantifying cyanobacteria biomass associated with moss is challenging because of the difficulty to separate bacteria colonies from the host plant. Attempts to separate cyanobacteria by shaking or sonicating in water were shown to be poorly efficient and repeatable. The techniques commonly used, microscopic counting and quantitative PCR (qPCR) are laborious and time-consuming. In aquatic and marine ecosystems, phycocyanin (PC), a photosynthesis pigment produced by cyanobacteria, is commonly used to monitor cyanobacteria biomass. In this study, we tested if PC extraction and quantification can be used to estimate cyanobacteria quantity inhabiting moss. We report that phycocyanin can be easily extracted from moss by freeze/thaw disturbance of cyanobacteria cells and can be quickly and efficiently measured by spectrofluorometry. We also report that phycocyanin extraction is efficient (high recovery), repeatable (relative SD < 13%) and that no significant matrix effects were observed. As for aquatic systems, the main limitation of cyanobacteria quantification using phycocyanin is the difference of cellular phycocyanin content between cyanobacteria strains, suggesting that quantification can be impacted by cyanobacteria community composition. Nonetheless, we conclude that phycocyanin extraction and quantification is an easy, rapid, and efficient tool to estimate moss-associated cyanobacteria number.

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Section: Introductionmentioning

confidence: 99%

Quantification of Moss-Associated Cyanobacteria Using Phycocyanin Pigment Extraction

2021

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“…This is in close agreement with the concentrations and CPC:PE ratios reported in P . galeate CCNP 1313 by Cegłowska et al (2020). Low concentrations of phycobiliproteins were also detected in Cyanothece sp.…”

Section: Resultsmentioning

confidence: 99%

Rapid analytical methods for the microalgal and cyanobacterial biorefinery: Application on strains of industrial importance

Palmer

Lawton

Kindt³

et al. 2021

MicrobiologyOpen

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To realize the potential of microalgae in the biorefinery context, exploitation of multiple products is necessary for profitability and bioproduct valorization. Appropriate analytical tools are required for growth optimization, culture monitoring, and quality control purposes, with safe, low‐tech, and low‐cost solutions favorable. Rapid, high‐throughput, and user‐friendly methodologies were devised for (a) determination of phycobiliproteins, chlorophylls, carotenoids, proteins, carbohydrates, and lipids and (b) qualitative and quantitative carotenoid profiling using UPLC‐PDA‐MSE. The complementary methods were applied on 11 commercially important microalgal strains including prasinophytes, haptophytes, and cyanobacteria, highlighting the suitability of some strains for coproduct exploitation and the method utility for research and industrial biotechnology applications. The UPLC method allowed separation of 41 different carotenoid compounds in <15 min. Simple techniques are described for further quantification and comparison of pigment profiles, allowing for easy strain selection and optimization for pigment production, with suitability for biotechnological or biomedical applications.

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“…Carotenoids provide photoprotection of the intracellular molecules in cyanobacteria [ 58 , 59 ]. However, the content of carotenoids is strain-specific and depends on different environmental/culture conditions, particularly light intensity [ 60 , 61 ]. In addition, accumulation of b-carotene could lead to the biosynthesis of abscisic acid (ABA) to regulate the stress-response mechanism during abiotic stress responses ( Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Light and Temperature on the Metabolic Profiling of Two Habitat-Dependent Bloom-Forming Cyanobacteria

et al. 2022

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Rapid proliferation of cyanobacteria in both benthic and suspended (planktonic) habitats is a major threat to environmental safety, as they produce nuisance compounds such as cytotoxins and off-flavors, which degrade the safety and quality of water supplies. Temperature and light irradiance are two of the key factors in regulating the occurrence of algal blooms and production of major off-flavors. However, the role of these factors in regulating the growth and metabolism is poorly explored for both benthic and planktonic cyanobacteria. To fill this gap, we studied the effects of light and temperature on the growth and metabolic profiling of both benthic (Hapalosiphon sp. MRB220) and planktonic (Planktothricoides sp. SR001) environmental species collected from a freshwater reservoir in Singapore. Moreover, this study is the first report on the metabolic profiling of cyanobacteria belonging to two different habitats in response to altered environmental conditions. The highest growth rate of both species was observed at the highest light intensity (100 μmol photons/m²/s) and at a temperature of 33 °C. Systematic metabolite profiling analysis suggested that temperature had a more profound effect on metabolome of the Hapalosiphon, whereas light had a greater effect in the case of Planktothricoides. Interestingly, Planktothricoides sp. SR001 showed a specialized adaptation mechanism via biosynthesis of arginine, and metabolism of cysteine and methionine to survive and withstand higher temperatures of 38 °C and higher. Hence, the mode of strategies for coping with different light and temperature conditions was correlated with the growth and alteration in metabolic activities for physiological and ecological adaptations in both species. In addition, we putatively identified a number of unique metabolites with a broad range of antimicrobial activities in both species in response to both light and temperature. These metabolites could play a role in the dominant behavior of these species in suppressing competition during bloom formation. Overall, this study elucidated novel insights into the effects of environmental factors on the growth, metabolism, and adaptation strategies of cyanobacteria from two different habitats, and could be useful in controlling their harmful effects on human health and environmental concerns.

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Characteristics of cyanobacterium Pseudanabaena galeata CCNP1313 from the Baltic Sea

Cited by 11 publications

References 67 publications

Quantification of Moss-Associated Cyanobacteria Using Phycocyanin Pigment Extraction

Quantification of Moss-Associated Cyanobacteria Using Phycocyanin Pigment Extraction

Rapid analytical methods for the microalgal and cyanobacterial biorefinery: Application on strains of industrial importance

Effects of Light and Temperature on the Metabolic Profiling of Two Habitat-Dependent Bloom-Forming Cyanobacteria

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