Characteristics of Cyclin B and its potential role in regulating oogenesis in the red claw crayfish (Cherax quadricarinatus)

Lm, Wang; Ww, Lv; D, Zuo; Dong, Zaijie; Yl, Zhao

doi:10.4238/2015.september.9.17

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…In total, 84 target genes were mapped to the four pathways (Supplementary Table S5 ); some of these genes had already been shown to be involved in the process of ovarian development in other organisms (Table 6 ). For example, the catalytic and regulatory subunits of mitogen-activated protein kinase kinase ( MAPKK ) 25 , extracellular signal-regulated kinase (ERK) 26 , 27 , fatty acid synthase ( FAS ) 28 , 29 , cyclin A, cyclin B 30 – 34 , and 14-3-3-like protein (14-3-3-like) 35 , 36 were previously shown to be involved.…”

Section: Resultsmentioning

confidence: 99%

Identification of MicroRNAs and Their Target Genes Associated with Ovarian Development in Black Tiger Shrimp (Penaeus monodon) Using High-Throughput Sequencing

Zhao

Fan

Qiu

2018

Sci Rep

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Plenty of evidence showing that microRNAs (miRNAs) post-transcriptionally regulate gene expression and are involved in a wide range of biological processes. However, the roles of miRNAs in ovarian development process remain largely unknown in shrimp. In the present study, high-throughput sequencing of small RNAs was performed to find specific miRNAs that are involved in ovarian development process in Penaeus monodon. Two small RNA libraries were constructed from undeveloped (UNDEV group) and developed (DEV group) ovarian tissues in P. monodon. In total, 43 differentially expressed miRNAs were identified between the two groups (P ≤ 0.05, |log2 ratio| ≥1), and their expression profiles were validated by qRT-PCR. In order to further clarify the functional roles of these differentially expressed miRNAs during ovarian development process, target gene prediction was performed. In total, 4,102 target genes of 43 miRNAs were predicted, then clustered by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database; only four specific pathways related to ovarian development were obtained (P < 0.05). Dual-luciferase reporter assays and integrated expression analysis were also conducted to further clarify the interaction between the miRNAs and their target mRNAs. This study provides important information about the function of miRNAs involved in ovarian developmental stages in P. monodon.

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Section: Resultsmentioning

confidence: 99%

Identification of MicroRNAs and Their Target Genes Associated with Ovarian Development in Black Tiger Shrimp (Penaeus monodon) Using High-Throughput Sequencing

Zhao

Fan

Qiu

2018

Sci Rep

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“…The observed different tissue distribution pattern might be the use of different species. The different expression levels in the testes and ovaries in different species are probably due to the samples obtained at different developmental stages (Wang, Lv, Zuo, Dong & Zhao, ; Wang et al., ). The β ‐catenin 1 was found to be expressed in almost all tissues in O. niloticus and C. carpio , suggesting that the canonical Wnt/β‐catenin signalling pathway is involved in the development of the almost all organs, as demonstrated in mammals (Barker & Clevers, ; Morita et al., ; Wu et al., ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of β-catenin 1 during the gonad development in the common carp (Cyprinus carpio )

et al. 2017

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β‐catenin gene is a pivotal gene for gonad development and maintenance of ovarian function in mammals. However, little is known about its expression and function in gonad development of fish. In this study, a complete cDNA (3342 bp) sequence of β‐catenin 1 was cloned from the common carp, Cyprinus carpio, by RACE PCR, which encodes a 780‐amino‐acid protein. Quantitative real‐time PCR demonstrated that β‐catenin 1 mRNA expressions were high in the testis and ovary tissue and the expression increased as the testes developed and the early stage ovaries developed. Western blot results revealed a single immunoreactive band with an estimated molecular weight of 90 kDa in testes. Immunohistochemistry analysis revealed that the β‐catenin 1 protein was concentrated mainly in the cytoplasm of early development stage of oocyte cells and in the cytomembrane of developing and mature sperm cells. 17β‐Ethinylestradiol injecting intraperitoneally into the fish decreased the relative β‐catenin 1 mRNA expression level except 1 μg/g 72 hr and 5 μg/g 48 hr of treatments in the ovary by real‐time PCR. These results suggest, for the first time, that β‐catenin 1 is an essential protein in gonad development and might be involved in ovarian early development of C. carpio.

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A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus

Wang

Shao³

et al. 2019

Mol Genet Genomics

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Characteristics of Cyclin B and its potential role in regulating oogenesis in the red claw crayfish (Cherax quadricarinatus)

Cited by 4 publications

References 23 publications

Identification of MicroRNAs and Their Target Genes Associated with Ovarian Development in Black Tiger Shrimp (Penaeus monodon) Using High-Throughput Sequencing

Identification of MicroRNAs and Their Target Genes Associated with Ovarian Development in Black Tiger Shrimp (Penaeus monodon) Using High-Throughput Sequencing

Characterization of β-catenin 1 during the gonad development in the common carp (Cyprinus carpio )

A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus

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