Characteristics of Dynamic Mass Redistribution of Epidermal Growth Factor Receptor Signaling in Living Cells Measured with Label-Free Optical Biosensors

Abstract

This paper reported the identification of a novel optical signature for epidermal growth factor (EGF) receptor signaling in human epidermoid carcinoma A431 cells mediated by EGF. The optical signature was based on dynamic mass redistribution (DMR) in living cells triggered by EGFR activation, as monitored in real time with resonant waveguide grating biosensors. Analysis of the modulation of the EGF-induced DMR signals by a variety of known modulators provided links of various targets to distinct steps in the cellular responses. Results showed that the dynamic mass redistribution in quiescent A431 cells mediated by EGF required EGFR tyrosine kinase activity, actin polymerization, and dynamin and mainly proceeded through MEK. The DMR signals obtained serve as integrated signatures for interaction networks in the EGFR signaling.

Modelling and mathematical analysis of the M$_{2}$ receptor-dependent joint signalling and secondary messenger network in CHO cells

“…the Ca 2+ -level or the cAMP concentration (Paredes et al, 2008;Hennen et al, 2013;Gabriel et al, 2003). In addition MODELLING AND MATHEMATICAL ANALYSIS OF SIGNALLING IN CHO CELLS 5 DMR has been introduced into the pharmaceutical field in order to specifically quantify the stimulation effect on the cytoskeleton (Fang et al, 2005;Schröder et al, 2011). The DMR is an optical biosensor based procedure and measures the shift in wavelength resulting from intracellular mass movement caused by rearrangement of cell organelles and transportation processes.…”

Evaluation of Bioactivity and Effect of Polymeric Stabilizers During Heat Treatment for the Unfolded Fraction of Human Epidermal Growth Factor

“…Epidermal growth factor (EGF) originating from ectodermal and mesodermal layers is a potent mitogenic factor for a variety of cultured cells [3][4][5][6]. In cosmetics, growth factors such as EGF are expected to participate in the generation of new skin from epidermal, epithelial and mesenchymal stem cells of the skin.…”

Modulating Cell-Cell Communication with a High-Throughput Label-Free Cell Assay

“…9,10 A resonant waveguide grating (RWG) sensor is able to measure reflective index change as a result of mass change Even though a mammalian cell typically has a height of >500 nm, its dynamic mass redistribution (DMR) in response to a stimulus can be detected and quantified with an RWG sensor if the DMR involves mass increase or decrease at the bottom portion of the cell perpendicular to the sensing surface. 11 A unique optical signature could be generated by monitoring DMR for a given cell type stimulated with a particular stimulus in a defined environment. This optical signature is robust and reproducible, and its usage as a novel measure for probing various cellular events has been intensively explored.…”

“…This optical signature is robust and reproducible, and its usage as a novel measure for probing various cellular events has been intensively explored. [11][12][13] In this study, we explored the use of an RWG optical sensor, Corning Epic System, for monitoring cell-cell communications in a high-throughput fashion. Among the many experimental model systems, we chose a cell-mediated cytotoxicity model system consisting of natural killer (NK) cells as effecter cells and two kinds of target cells: (1) Chinese hamster ovarian (CHO) cells overexpressing intercellular adhesion molecule 1 (CHO-ICAM1) and (2) cervical cancer HeLa cells because of the extensive knowledge available from the literature.…”