Characteristics of Extended-Spectrum β-Lactamase–ProducingEscherichia coliin Retail Meats and Shrimp at a Local Market in Vietnam

Abstract

This is the first report of the characteristics of ESBL-producing E. coli in retail foods in a local city in Vietnam. Our findings indicate that retail foods are contaminated with ESBL-producing E. coli, of which many were MDR. Further monitoring and public health efforts targeting food administration are needed to control the spread of ESBL-producing bacteria in communities.

Widespread dissemination of extended-spectrum β-lactamase-producing, multidrug-resistant Escherichia coli in livestock and fishery products in Vietnam

“…ESBL-E. coli belonging to groups B1 and A accounted for approximately two-thirds of all ESBL-E. coli isolates from the food samples, followed by those belonging to group D. In contrast, ESBL-E. coli belonging to group B2 accounted for only 8.6 % of all ESBL-E. coli isolates from the food samples. This order of the most to least prevalent phylogenetic groups observed in the present study was similar to that observed in a recent study conducted in Nha Trang, Vietnam (Le et al 2015). In addition, a similar phylogenetic profile was observed for ESBL-E. coli isolates from chicken samples in the Netherlands, with the highest percentage (44 %) of isolates belonging to group B1, followed by groups A (28 %), D (23 %), and B2 (2 %) (Kluytmans et al 2013).…”

“…The results of this study are similar to those of previous studies conducted in other regions of Vietnam (Cortés et al 2010;Leverstein-van Hall et al 2011;Le et al 2015). There was a significant difference in the multi-ESBL genes encoded by the isolates obtained from poultry compared to those encoded by isolates from pork, shrimp, and fish samples (p < 0.001).…”

“…A previous study conducted in Nha Trang, a mediumsized coastal city in southern Vietnam, showed that the prevalence of ESBL-E. coli in poultry, pork, and shrimp products was 58.7, 32, and 18.3 %, respectively (Le et al 2015). The results of both the present and previous studies showed a higher prevalence of ESBL-E. coli in poultry products than in other food products in both areas of Vietnam.…”

“…Bacterial DNA was extracted by boiling the bacterial suspension in tris(hydroxymethyl)aminomethane-EDTA buffer (pH 8.0). The extracted DNA was used as the template for amplification by multiplex PCR using primers specific for genes encoding enzymes belonging to the TEM, SHV, CTX-M-1, CTX-M-2, CTX-M-9, and CTX-M-8/CTX-M-25 groups (Le et al 2015). The PCR conditions used were as follows: initial denaturation at 95°C for 5 min, followed by 25 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 90 s, and extension at 72°C for 90 s, with a final extension at 68°C for 10 min.…”

Drug-resistant bacteria in frozen and fresh marine shrimp

“…Food contamination with multidrug-resistant bacteria is considered a potential source for the wide dissemination of resistant-bacteria in communities (Le et al, 2015). Resistance to 16 drugs (ampicillin, ciprofloxacin, polymixin B, cefixime, amoxicillin, ceftriazone, penicillin, chloramphenicol, trimethoprime-sulfomethoxazole, gentamycin, nalidixic acid, kanamycin, vancomycine, erythromycin, tetracycline, streptomycin) in pathogenic bacteria isolated from shrimps was reported by Noor et al (2014), indicating that these invertebrates can be vehicles in the transmission of drug resistant strains.…”