An atmospheric boundary layer wind tunnel study was performed to determine the overall horizontal wind load on multi-span duo-pitch greenhouses. The results of this study are intended for the stability design of inflexible cladding system greenhouses (designated as Class A in EN 13031-1) with roof pitch angles of 23-24°and ridge heights of 7.5-8 m. Both static force and fluctuating pressure measurements were performed to determine the overall horizontal wind force. The results obtained with the static force measurements show that the overall horizontal wind force increases linearly with increasing number of spans. The overall horizontal wind load determined with the mean pressure coefficients derived from the fluctuating pressure measurements was found to be in good agreement with the static force measurements. An investigation into the correlation of the roof pressures showed that these pressures are relatively little correlated. The overall horizontal force on the roof of multi-span buildings reduces significantly because of this lack of correlation. Accounting for the lack of correlation between the roof pressures on a 30-span model resulted in a 65% reduction of the peak horizontal wind force on the roof. A comparison was made of the overall horizontal wind forces determined in the current study and calculated with European codes for wind load assessment on greenhouses. EN 13031-1 provides non-conservative outcomes for the overall horizontal wind force on the investigated duo-pitch greenhouse type with more than 30 spans. On the other hand, EN 1991-1-4 is increasingly conservative with larger number of spans.Keywords: static wind loads, multi-span duo-pitch greenhouses, stability design, wind tunnel measurements, force and pressure coefficients, correlation of wind pressures, size factor, dynamic coefficient HIGHLIGHTS 1. The finding that the overall horizontal wind force on multi-span duo-pitch greenhouses increases linearly with increasing number of spans. 2. The quantification of the lack of correlation between roof faces on multi-span duo-pitch buildings, demonstrating the importance of this effect for the overall horizontal wind force. 3. The identification of the need for positive values, besides negative values, for the pressure coefficients in EN 1991-1-4 and EN 13031-1 on wind-facing roof faces beyond the first roof span. 4. The conclusion that EN 13031-1 provides non-conservative outcomes for the overall horizontal wind force on duo-pitch greenhouses (with roof pitch angle 23-24°) larger than 30 spans, and that, beyond 10 spans, EN 1991-1-4 is increasingly conservative with larger number of spans.