Characteristics of frozen epididymal spermatozoa from stallions that died 12 to 36 hours after colic surgery

Gloria, Alessia; Carluccio, Augusto; Petrizzi, Lucio; Noto, Federico; Contri, Alberto

doi:10.1016/j.theriogenology.2015.09.006

Cited by 8 publications

(3 citation statements)

References 32 publications

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“…The sperm kinematics of frozen-thawed ejaculated sperm were higher than corpus and cauda epididymal sperm, contrary to previous reports in horses [2,3,45,46], pigs [47], and goats [48], which observed that the motility parameters and PMI after thawing were similar in ejaculated and epididymal sperm [3]. Moreover, the TM and PM of cauda epididymal sperm were lower than that described by Papa et al [1] and Monteiro et al [3]; probably, the age factor may have influenced the results as stallions aged between 34 and 38 months were used.…”

Section: Discussioncontrasting

confidence: 96%

Heterologous Oviductal Cells Binding Capacity of Cryopreserved Equine Ejaculated and Epididymal Spermatozoa

Carneiro

Monteiro

Martin

et al. 2017

Journal of Equine Veterinary Science

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This study investigated the binding capacity of equine spermatozoa (both ejaculated and from different epididymal regions) to the oviductal epithelial cells (OECs) culture, before and after cryopreservation, using an oviduct explant assay. Ejaculated and epididymal sperm from caput, corpus, and cauda of 10 stallions were diluted and submitted to freezing process. Fresh and frozen-thawed sperm were evaluated for sperm kinematics, plasma membrane integrity (PMI) and incubated with oviduct explants. The cryopreservation process decreased significantly the sperm motility parameters of ejaculated sperm, and corpus and cauda epididymal sperm (P < .05). The percentage of PMI was significant higher in fresh samples versus frozen-thawed samples, in all analyzed groups (P < .05). Binding of ejaculated spermatozoa to oviduct epithelium was significantly higher than caput, corpus, or cauda epididymal sperm (P < .05). The caput epididymal sperm showed no binding capacity to oviduct explants; thus, significantly more sperm recovered from the corpus and cauda epididymis were bound to OEC compared to caput epididymal sperm (P <.05). No differences were observed in ejaculated and epididymal sperm before and after cryopreservation (P > .05). In conclusion, the ejaculated sperm has higher binding capacity than epididymal sperm, suggesting that the seminal plasma plays an important role in the establishment of the oviductal sperm reservoir. The cryopreservation process did not affect the binding capacity of ejaculated or epididymal spermatozoa to oviductal epithelium.

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Section: Discussioncontrasting

confidence: 96%

Heterologous Oviductal Cells Binding Capacity of Cryopreserved Equine Ejaculated and Epididymal Spermatozoa

Carneiro

Monteiro

Martin

et al. 2017

Journal of Equine Veterinary Science

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“…Epididymal spermatozoa can be successfully retrieved after 48 hours and viable pregananices can be obtained (Stawicki et al, 2016). Motility parameters of epididymal spermatozoa are similar between routinely castrated healthy stallions and those that died because of acute illness (Gloria et al, 2016).…”

Section: Resultsmentioning

confidence: 98%

Ampullar and Ductus Deferens Aspiration Increases the Total Number of Viable Spermatozoa Recovered from Stallion Extragonadal Sperm Reserves

Rus

Groza

PALL

et al. 2022

IJAR

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Background: Equine spermatozoa acquire maturation and fertilization capacity during their passage from the testis towards the cauda epididymis and are concurrently stored in the cauda epididymis and ampullae of the ductus deferens. In case of sudden death or emergency castration, epididymal spermatozoa can be harvested and successfully preserved. Ampullar aspiration was not previously described, even though the ampullae of the ductus deferens and the ductus deferens are valuable sources of mature spermatozoa in equines. The aim of this study was to describe a technique to collect spermatozoa from the ampullae of the ductus deferens and the ductus deferens of stallions during routine castration and to compare kinematic parameters of these spermatozoa to spermatozoa harvested from the cauda epididymis of the same stallion. Methods: Fourteen ampullae of the ductus deferens were successfully aspirated during the routine castration of 10 stallions, followed by epididymal sperm harvest. Concentration and motility parameters were assessed, computer-assisted and were compared to epididymal spermatozoa. Result: Ampullar and ductus deferens spermatozoa increased the total number of spermatozoa recovered by approximately 6%. Progressive motility (PM), velocity of the average path (VAP), velocity of the curved line (VCL) and linearity (LIN) did not differ significantly (P≤0.05) between the ampullar spermatozoa (AS) and the epididymal spermatozoa (ES). Total motility (TM) was significantly lower (P= 0.04) in the AS group.

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“…Barbiturate verminderten die Spermienqualität bei Hunden [6] und Katzen [16], aber nicht bei Ratten [33]. Auch bei Pferden mit schweren Erkrankungen wie Koliken und nach Medikation wurden Nebenhodenspermien mit guter Qualität konserviert [10]. Allerdings sollten die Hoden und Nebenhoden vor der Euthanasie entnommen werden [7], sofern das aus Tierschutzsicht vertretbar ist.…”

Section: Samenkonservierungunclassified

Geburt eines Fohlens nach Besamung mit tiefgefrorenen Nebenhodenspermien und zeitgleicher intrauteriner Verabreichung von homologem Seminalplasma

Neuhauser

Gösele

Handler

2019

Tierarztl Prax Ausg G

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ZusammenfassungDie Tiefgefrierkonservierung von Nebenhodenspermien stellt eine gute Möglichkeit zur Erhaltung der Genreserve wertvoller Vatertiere dar, wenn ein plötzlicher Unfall eine herkömmliche Samengewinnung unmöglich macht. Im vorliegenden Fall wird von der Geburt eines gesunden Fohlens berichtet, das aus der Besamung mit Nebenhodenspermien entstand. Die Hoden und Nebenhoden wurden in Allgemeinanästhesie entfernt und gekühlt über Nacht in das Labor verbracht. Durch retrograde Spülung der Nebenhodenschwänze konnten 17,79 × 109 Spermien konserviert werden. Eine fruchtbare Stute wurde 1 Jahr später mit tiefgefrorenen Nebenhodenspermien tief intrauterin besamt. Im 1. Zyklus wurde die Stute nicht tragend. Im 2. Zyklus wurde homologes Seminalplasma zusammen mit den Nebenhodenspermien in den Uterus verbracht und 14 Tage nach der Ovulation konnte eine Trächtigkeit festgestellt werden. Nach einer Graviditätsdauer von 334 Tagen wurde ein gesundes Hengstfohlen geboren. Die Vorbereitung von Hoden und Nebenhoden nach einem Unfall oder Todesfall für die weitere Bearbeitung im Labor und die einzelnen Arbeitsschritte für die Aufbereitung von Nebenhodenspermien werden beschrieben.

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Characteristics of frozen epididymal spermatozoa from stallions that died 12 to 36 hours after colic surgery

Cited by 8 publications

References 32 publications

Heterologous Oviductal Cells Binding Capacity of Cryopreserved Equine Ejaculated and Epididymal Spermatozoa

Heterologous Oviductal Cells Binding Capacity of Cryopreserved Equine Ejaculated and Epididymal Spermatozoa

Ampullar and Ductus Deferens Aspiration Increases the Total Number of Viable Spermatozoa Recovered from Stallion Extragonadal Sperm Reserves

Geburt eines Fohlens nach Besamung mit tiefgefrorenen Nebenhodenspermien und zeitgleicher intrauteriner Verabreichung von homologem Seminalplasma

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