Characteristics of Groundwater Microbial Community Composition and Environmental Response in the Yimuquan Aquifer, North China Plain

Abstract

To explore the intricate interrelationship between groundwater microbial communities and hydrochemical characteristics in the Mancheng District, Baoding City, samples from karst groundwater and porous groundwater were collected. Hydrochemical analysis and the 16s RNA gene V4-V5 region sequencing method were used to detail the water environmental characteristics and microbial community distribution pattern in the study area. The hydrochemical results reveal notable differences in major ion contents and other hydrochemical indices between karst water and porous water. Microbial community analysis demonstrates that Proteobacteria, Actinobacteria, and Firmicutes are the dominant taxa in both types of water bodies, albeit with significant variations in their proportions across distinct water bodies. The observed disparities in microbial communities between karst water and porous water underscore the influence of environmental factors such as ion concentration and pH on microbial distribution. Redundancy Analysis (RDA) indicates notable associations between microbial community composition and environmental factors in both types of groundwater environments. Studying the distribution pattern of microbial communities and the connection with environmental factors in two types of groundwater lays a crucial foundation for interpreting the functional roles of microbes in groundwater ecosystems.