Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying Arthrobacter nicotianae D51 Strain in the Presence of Copper

Abstract

A heterotrophic nitrification and aerobic denitrification bacterium, strain D51, was identified as Arthrobacter nicotianae based on morphological, phospholipid fatty acids (PLFAs), and 16S rRNA gene sequence analyses. Further tests demonstrated that strain D51 had the capability to use nitrite, nitrate, or ammonium as the sole nitrogen source in the presence of Cu2+. The maximum removal efficiencies of nitrite, nitrate and ammonium were 68.97%, 78.32%, and 98.70%, respectively. Additionally, the maximum growth rate and denitrification capacity of this strain occurred in the presence of 0.05 mg·L−1 of Cu2+.However, the growth and aerobic denitrification capacity were intensively inhibited by Cu2+ at ≥0.1 mg·L−1. Moreover, gas chromatography indicated that a portion of the nitrogen was transformed into N2O when the nitrite, nitrate, and ammonium were separately used as the sole nitrogen source. This is the first study of the nitrification and denitrification ability of Arthrobacter nicotianae under aerobic conditions, and the first experiment to investigate the impact of Cu2+ concentration on the growth and denitrification ability of this bacteria. The results presented herein extend the known varieties of heterotrophic nitrifying–aerobic denitrifying bacteria and provide useful information regarding the specific bacteria for nitrogen bioremediation of industrial wastewater containing Cu2+.

Heterotrophic aerobic denitrification by novel bacterium Georgenia daeguensis ARB2 for treatment of nitrate contaminated waters

“…was observed in the presence of Cr 6+ stress (10 mgL −1 ) (Sun et al 2016). Similarly, Cu 2+ at a concentration > 0.1 mgL −1 inhibited growth and denitrification by Arthrobacter nicotianae (Cai et al 2019). Thus, strain ARB2 can be used in nitrate removal from wastewaters where salt and metal concentrations are low or within permissible limits.…”

Role of heterotrophic aerobic denitrifying bacteria in nitrate removal from wastewater

“…An increase in copper concentration from 0·01, to 0·1 mmol l −1 resulted in decreased denitrification from 99·8 to 16·3% (Lu et al ). A study conducted by Cai et al () showed the growth and denitrification ability of Arthrobater nicotianae in the presence of Cu 2+ . Cu 2+ at a concentration of >0·1 mg l −1 inhibited both growth and denitrification.…”

Spatial and temporal conversion of nitrogen using Arthrobacter sp. 24S4–2, a strain obtained from Antarctica

“…Many members of the genus Arthrobacter have been found to have the ability to reduce nitrate, such as Arthrobacter oryzae , Arthrobacter pokkalii , Arthrobacter globiformis , Arthrobacter arilaitensis ( Pinar and Ramos, 1997 ; Kageyama et al, 2008 ; He and Li, 2016 ; Krishnan et al, 2016 ). And it has been shown that the reduction of nitrate by Arthrobacter is usually considered to be through denitrification ( He et al, 2017 ; Zhong et al, 2018 ; Cai et al, 2019 ). However, nitrate reduction to ammonium by Arthrobacter has rarely been reported.…”