Characteristics of histamine release from isolated rat mast cells: Differences when various ligands are used to induce secretion

Healicon, Richard; Foreman, J. C.

doi:10.1007/bf01973829

Cited by 4 publications

(6 citation statements)

References 17 publications

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“…The T1/2 for inactivation by Con A (11.2 min) that we have found is considerably longer than that which has been reported (1.0 min) for antigen [26]. This agrees with the suggestions of others [27,28] that various immunologic agents may have different modes of action.…”

Section: Discussionsupporting

confidence: 88%

Mast cell secretion: differences between immunologic and non-immunologic stimulation

1986

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Non-immunologic and immunologic stimulation of mast cells have been compared. Non-immunologic stimulation relys heavily on cellular Ca, is unaffected by neuraminidase treatment, shows a rapid inactivation, and elicits no increase in the incorporation of 3H-methyl groups into the lipid fraction. In contrast, stimulation by immunologic agents relys primarily on extracellular Ca, is inhibited by neuraminidase treatment, shows a comparatively slow rate of inactivation, and causes a significant increase in the incorporation of 3H-methyl groups into the lipid fraction. We found no evidence of cross-inactivation or desensitization between immunologic and non-immunologic agents. However, pretreatment of mast cells with neurotensin desensitized them to subsequent stimulation by compound 48/80. Our results support the hypothesis that immunologic and non-immunologic agents activate exocytotic mast cell secretion via separate mechanisms.

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Section: Discussionsupporting

confidence: 88%

Mast cell secretion: differences between immunologic and non-immunologic stimulation

1986

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“…As we have previously reported [5] there are quantitative differences in the response of rat peritoneal mast cells to three different modes of cross-linking the Fc-receptor for IgE: antigen (ovalbumin), anti-IgE and dimerized IgE. Possible explanations for these differences were investigated in the work reported here.…”

Section: Discussionmentioning

confidence: 77%

“…In a previous paper [5] we reported that the rate of release due to anti-IgE was slower than that due to ovalbumin. Both agents were used at supra-maximal doses.…”

Section: Resultsmentioning

confidence: 93%

“…In a previous paper [5] the characteristics of histamine release induced by antigen and anti-IgE were investigated and differences were found in the rate of release and the rate of desensitization induced by these two ligands. This paper reports further work aimed at characterizing the differences between these stimuli and explores possible mechanisms by which these differences may be mediated.…”

Section: Introductionmentioning

confidence: 99%

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Rat mast cell activation and inactivation: Differences when various ligands are used to induce secretion

Healicon

Foreman

1985

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The relationship between rat peritoneal mast cell activation and inactivation (desensitization) was studied for a variety of stimuli acting via IgE and IgG receptors on the cell surface. Anti-IgE, antigen (ovalbumin), anti-IgG1, anti-IgG2a and dimers, trimers and higher oligomers of IgE were used to induce histamine release from rat mast cells. All produced similar characteristics of cell activation, with a rapid rate of histamine release from the cells, release being 90% complete within 5 minutes and with calculated doubling times between 21.6 +/- 3.6 s (+/- SEM) for ovalbumin and 93.0 +/- 18.6 s (+/- SEM) for anti-IgG1. The characteristics of inactivation, however, varied with the releasing agent used. Of all the stimuli used only ovalbumin showed a rapid rate of desensitization (t 1/2 = 330 +/- 34.8 s) which correlated with the cessation of histamine release. The other stimuli showed slow rates of desensitization (t 1/2 between 1068 +/- 40.2 s for dimer and 3576 +/- 660 s for anti-IgE) even though the rate of release was rapid. Thus, although these stimuli are thought to be stimulating the cells by cross-linking of either IgE or IgG receptors, the difference in subsequent response of the cells would indicate that the transduction mechanism bringing about release can distinguish between these various stimuli. Also, it seems that the idea that duration of histamine release is determined by the rate of desensitization may need revising as with most of these stimuli, release has terminated when the cells are still in a fully activated state.

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“…These different kinetics in cal cium response may reflect a more effective cross-linking of surface antibody IgE by a multivalent ligand (mite al lergen) than by the functionally bivalent anti-human IgE. These results may be compared to the difference in the rate of release of histamine induced cither by antigen or anti-IgE in rat mast cells [30] or in human basophils [31], Indeed, the release of histamine by antigen occurs with a shorter lag period and a more rapid rate than seen with anti-IgE.…”

Section: Discussionmentioning

confidence: 99%

Expression of the Alpha Chain of Human FcεRI in Transfected Rat Basophilic Leukemia Cells: Functional Activation after Sensitization with Human Mite-Specific IgE

Taudou

Varin‐Blank²,

Jouin³

et al. 1993

Int Arch Allergy Immunol

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The actual dilemma in studying the binding and triggering capacity of IgE from allergic patients is the lack of cultured basophils or mast cell analogs of human origin. Human IgE binds with exquisite species specificity to the high affinity IgE receptor (FcεRI) expressed on the surface of these cells. In rodents this receptor has been characterized as a tetrameric plasma membrane protein composed of an IgE-binding α chain, a β chain and two disulfide-linked γ chains. In order to establish a cell line expressing the α chain of human FcεRI which can be triggered with IgE from human patients and specific allergen, we transfected the cDNA coding for the human α subunit into rat basophilic leukemia cells. The resulting transfectants express the human α chain on the cell surface in the form of a hybrid complex associated with endogenous rat γ chains. After sensitization with human IgE from mite-specific patients, the transfectant produces a calcium response upon incubation with allergen. The established cell line can be used as a model system to study the mechanism of mast cell triggering through IgE from allergic patients.

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Characteristics of histamine release from isolated rat mast cells: Differences when various ligands are used to induce secretion

Cited by 4 publications

References 17 publications

Mast cell secretion: differences between immunologic and non-immunologic stimulation

Mast cell secretion: differences between immunologic and non-immunologic stimulation

Rat mast cell activation and inactivation: Differences when various ligands are used to induce secretion

Expression of the Alpha Chain of Human FcεRI in Transfected Rat Basophilic Leukemia Cells: Functional Activation after Sensitization with Human Mite-Specific IgE

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