Characteristics of immunobiochemical and morphological indicators of blood in cows with pyometra

Abstract

Inflammatory diseases of the uterus are one of the main causes of decreased fertility and infertility in dairy cows. The aim of the study was to assess changes in the immunobiochemical and morphological parameters of the blood of dairy cows with pyometra. 20 cows with pyometra (G1) and 15 cows without visualized reproductive pathology (G2) were selected using transrectal ultrasound. The blood test showed that in G1 there was the decrease in the level of total protein to 73.69 ± 1.06 gr/l, which was 11.1% lower (P<0.001) than in the G2. The concentration of albumin in G1 was 34.23 ± 0.56 gr/l, which was 17.5% less (P<0.001) in comparison with the G2. The creatinine level in the G1 was 19.2% higher (P<0.01) and it amounted to 98.30 ± 4.92 μmol/l, and the urea level was 6.34 ± 0.37 mmol/l, which was 1,6 times higher (P<0.001). The AST activity in the cows from G1 was 100.06 ± 5.53 units/l, which was 19.4% higher (P<0.05), with the ALT activity was the same. The amount of low and medium molecular weight substances adsorbed on the surface of erythrocytes in the G1 was 15.4% higher, and those associated with plasma albumin was 21.8% higher (P<0.001). The immunological indicators in the G1 were characterized by the increase in the total immunoglobulins level by 63.2% (P<0.001), the concentration of which was 20.58 ± 1.13 mg/l. The content of circulating immune complexes of large and small sizes in the blood was 11.82 ± 0.88 optical density and 1.84 ± 0.06 optical density respectively, which was 2 times higher (P<0.001) than the indicator of healthy cows. The morphological pattern of blood in the G1 was characterized by leukocytosis ‐ 10.08 ± 0.86 *109/l (the increase in the concentration of leukocytes by 17.2%, P<0.05), the decrease in the concentration of erythrocytes by 4.7% (P<0, 05) ‐ 6.35 ± 0.10 *1012/l and platelets by 42.8% (P<0.05) ‐ 176.88 ± 22.16 *109/l. There was qualitative redistribution of white blood cells: the increase in the concentration of neutrophils by 6.6%, eosinophils by 6.3 times (P<0.001), the decrease in lymphocytes by 15.8% (P<0.001). Thus, associated with pyometra in dairy cows, changes in protein and nitrogen metabolism are observed: the level of total protein is decreased, the level of globulins is increased, the level of urea and creatinine is increased, and the AST activity is increased. The immunological indicators are characterized by the increase in the level of total immunoglobulins and circulating immune complexes, and in the morphological blood pattern of the cows with pyometra, one can observe leukocytosis with granulocytic cells predominating over agranulocytes.

Bacteriological examination of pathological material from cows

“…Проникновение в половую систему патогенов из внешней среды приводит к развитию послеродового эндометрита. [3,4].…”