Characteristics of Large Animal Models for Current Cell-Based Oral Tissue Regeneration

Abstract

The recent advances in the field of cell-based therapeutics open promising perspectives for oral tissue regeneration. The development of large animal models, which overcome the limits of the rodent models and allow to emulate clinical situations, is crucial for the validation of regenerative strategies to move towards clinical application.Currently, porcine, canine and ovine models are the mainly developed for oral regeneration and their specific characteristics have an impact on the outcomes of the studies.Thus, this systematic review investigates the application of porcine, canine and ovine models in present cell-based oral regeneration, according to the species characteristics and the targeted tissue to regenerate.A customized search of PubMed, EMBASE, Scopus, and Web of Science databases, from January 2015 to March 2020 was conducted. Relevant articles about cell-based oral tissues engineering in porcine, canine and ovine models were evaluated. Among the evaluated articles, fifty-eight relevant studies about cell-based oral regeneration in porcine, canine and ovine models matched the eligibility criteria and were selected for full analysis.Porcine models, the most similar specie with humans, were mostly used for bone and periodontium regeneration; tooth regeneration was reported only in pig except for one study in dog.Canine models were the most transversal models, successfully involved for all oral tissues regeneration and notably in implantology. However, differences with humans and ethical concerns affect the use of these models.Ovine models, alternative to porcine and canine ones were mainly used for bone and, scarcely, for periodontium regeneration. The anatomy and physiology of these animals restrain their involvement.

3D-printed porous Ti6Al4V scaffolds for long bone repair in animal models: a systematic review

“…At the same instance, it should be kept in mind that every large animal model also has its pros and cons. For instance, dogs offer an optimal model for assessing Ti64 scaffold’s effectiveness; however, due to ethical concerns and increased public scrutiny their application in animal research has been declining [ 72 ]. An adult sheep has similarities in weight, metabolism, and bone remodeling rates to that of humans and could be considered ideal for testing the scaffold and transferring the findings to a clinical setting [ 73 ].…”

Kappa-carrageenan-Functionalization of octacalcium phosphate-coated titanium Discs enhances pre-osteoblast behavior and osteogenic differentiation

“…Recent advances in the field of cell-based therapeutics provide new perspectives for oral tissue regeneration. The development of large animal models, which overcome the limitations of rodent models and allow to emulate clinical situations, is crucial for the validation of regenerative strategies to move toward clinical application ( Mangione et al, 2022 ). Canine models are successfully used in all oral tissue regeneration, notably implantology studies ( Uijlenbroek et al, 2022 ).…”

A critical analysis of research methods and biological experimental models to study pulp regeneration

“…Nonetheless, pulp regeneration studies that specifically address the effects of pulp capping materials and/or pulpotomies are still possible in rodents, as some studies have proven (Minic et al, 2021). However, for full pulp regeneration studies, these species present too many disadvantages to provide meaningful data, leading to the preference for larger animal models (Mangione et al, 2021; Nakashima et al, 2019). Among these larger models, the literature has studies that use various animal groups (e.g., sheep, minipig, ferret, and dog), each with advantages and disadvantages.…”

“…Sheep ( Ovis aries ) studies are not very common but show good results in pulp regeneration (Altaii et al, 2017) due to the animal size and shape of the teeth. There are disadvantages to these studies, such as the fact that sheep are ruminants, which can affect the oral pH (Mangione et al, 2021), and the fact that not many dental studies used this species. Minipig ( Sus scrofa ) models are another emerging alternative for the study of pulp regeneration.…”