Characteristics of leaf shapes among two parental Drosera species and a hybrid examined by canonical discriminant analysis and a hierarchical Bayesian model

Hoyo, Yuri; Tsuyuzaki, Shiro

doi:10.3732/ajb.1200510

Cited by 12 publications

(12 citation statements)

References 38 publications

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“…In contrast to other studies, referring to hybrids as either intermediate phenotypes or mosaics of their parents [3], [46], [86], [87] our cloned ITS data underlined the remarkable incongruence between the genotype and leaf morphology of the Ranunculus cassubicifolius × notabilis triploid hybrid. While including two genome copies from the maternal parent, the hybrid retained the clearly distinct paternal-like leaf morphology.…”

Section: Discussioncontrasting

confidence: 99%

ITS Polymorphisms Shed Light on Hybrid Evolution in Apomictic Plants: A Case Study on the Ranunculus auricomus Complex

et al. 2014

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The reconstruction of reticulate evolutionary histories in plants is still a major methodological challenge. Sequences of the ITS nrDNA are a popular marker to analyze hybrid relationships, but variation of this multicopy spacer region is affected by concerted evolution, high intraindividual polymorphism, and shifts in mode of reproduction. The relevance of changes in secondary structure is still under dispute. We aim to shed light on the extent of polymorphism within and between sexual species and their putative natural as well as synthetic hybrid derivatives in the Ranunculus auricomus complex to test morphology-based hypotheses of hybrid origin and parentage of taxa. We employed direct sequencing of ITS nrDNA from 68 individuals representing three sexuals, their synthetic hybrids and one sympatric natural apomict, as well as cloning of ITS copies in four representative individuals, RNA secondary structure analysis, and landmark geometric morphometric analysis on leaves. Phylogenetic network analyses indicate additivity of parental ITS variants in both synthetic and natural hybrids. The triploid synthetic hybrids are genetically much closer to their maternal progenitors, probably due to ploidy dosage effects, although exhibiting a paternal-like leaf morphology. The natural hybrids are genetically and morphologically closer to the putative paternal progenitor species. Secondary structures of ITS1-5.8S-ITS2 were rather conserved in all taxa. The observed similarities in ITS polymorphisms suggest that the natural apomict R. variabilis is an ancient hybrid of the diploid sexual species R. notabilis and the sexual species R. cassubicifolius. The additivity pattern shared by R. variabilis and the synthetic hybrids supports an evolutionary and biogeographical scenario that R. variabilis originated from ancient hybridization. Concerted evolution of ITS copies in R. variabilis is incomplete, probably due to a shift to asexual reproduction. Under the condition of comprehensive inter- and intraspecific sampling, ITS polymorphisms are powerful for elucidating reticulate evolutionary histories.

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Section: Discussioncontrasting

confidence: 99%

ITS Polymorphisms Shed Light on Hybrid Evolution in Apomictic Plants: A Case Study on the Ranunculus auricomus Complex

et al. 2014

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“…Triploid hybrids from D. spatulata and D. rotundifolia have been produced by artificial crossing and are sterile. Natural triploid hybrids occur occasionally in the field and are usually sterile (Hoyo and Tsuyuzaki 2013). The fertility of these triploid hybrids can be rescued by chromosome doubling using colchicine (Campos et al 2009).…”

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confidence: 99%

Breeding and Cytogenetic Characterizations of New Hexaploid Drosera Strains Colchicine-Induced from Triploid Hybrid of D. rotundifolia and D. spatulata

et al. 2016

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Summary An in vitro technique was connected with application to produce colchicine-induced hexaploid from the artificial-crossing triploid hybrid of Drosera rotundifolia and D. spatulata. The colchicine-treated plants of artificial-crossing triploid hybrid showed morphologically mutated characteristics. Three hexaploid strains by chromosome doubling were produced after screening of clones treated with 0.05 and 0.1% colchicine solutions for one or three days. Each of the strains had 2n=60 with 20 middle size and 40 small size chromosomes (2n=6x=20M+40S). The stomata guard cell sizes of colchicine-induced hexaploid and the wild hexaploid species D. tokaiensis were larger than that of the artificial-crossing triploid hybrid. Flow cytometry analysis showed that 2C DNA-values of D. rotundifolia (2n=2x=20M), D. spatulata (2n=4x=40S) and D. tokaiensis (2n=6x= 20M+40S) were 2.73, 1.41 and 3.74 pg, respectively. In contrast, the artificial-crossing triploid hybrid (2n=3x=10M+20S) and colchicine-induced hexaploid (2n=6x=20M+40S) were 2.31 and 4.41 pg, respectively. The genome size of the artificial-crossing triploid hybrid (2.31 pg) was nearly half the size of the colchicineinduced hexaploid (4.41 pg). Compared to the colchicine-induced hexaploid, the genome size of D. tokaiensis was unexpectedly small, even though they contained the same genome constitutions.

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“…For seedling survival and growth analysis, seedlings where the maximum leaf length >3 mm were used, following seedling identification (Hoyo and Tsuyuzaki 2013). Hierarchical Bayesian models (HBMs) estimated the determinants on density, recruitment and survival for the two species.…”

Section: Discussionmentioning

confidence: 99%

“…rotundifolia and D. anglica became established on the peatland where peat-mining was conducted ca. 39 years ago (Hoyo and Tsuyuzaki 2013). Five 300×40 cm plots divided into thirty 20×20 cm cells in areas where the two Drosera species were established.…”

Section: Field Census and Seed Preparationmentioning

confidence: 99%

Habitat Differentiation Between Drosera anglica and D. rotundifolia in a Post-Mined Peatland, Northern Japan

Hoyo

Tsuyuzaki

2014

Wetlands

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1The preservation of endangered species requires clarifying habitat preferences through 2 survival, growth and competitive ability. The determinants of habitat differentiation between 3 the endangered species, Drosera anglica, and a widespread congener, D. rotundifolia, were 4 compared. The effects of water level, Sphagnum mats and overstory vascular plants on 5 Drosera distribution, recruitment and survival were monitored at a previously mined 6 Sphagnum peatland. Seedling transplant experiments were conducted using different water 7 levels. Seed-sowing experiments were conducted using different light intensities in three

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Characteristics of leaf shapes among two parental Drosera species and a hybrid examined by canonical discriminant analysis and a hierarchical Bayesian model

Abstract: The HBM enabled convenient, nondestructive measurements for species identification by considering nonlinear relationships between morphological traits and measurement error. The HBM is likely to be applicable to various follow-up studies, as well as species conservation.

Cited by 12 publications

References 38 publications

ITS Polymorphisms Shed Light on Hybrid Evolution in Apomictic Plants: A Case Study on the Ranunculus auricomus Complex

ITS Polymorphisms Shed Light on Hybrid Evolution in Apomictic Plants: A Case Study on the Ranunculus auricomus Complex

Breeding and Cytogenetic Characterizations of New Hexaploid <i>Drosera</i> Strains Colchicine-Induced from Triploid Hybrid of <i>D. rotundifolia</i> and <i>D. spatulata</i>

Habitat Differentiation Between Drosera anglica and D. rotundifolia in a Post-Mined Peatland, Northern Japan

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