Characteristics of magnetically separated rat tracheal epithelial cell populations

Ford, John; Terzaghi-Howe, M.

doi:10.1152/ajplung.1992.263.5.l568

Cited by 8 publications

(9 citation statements)

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“…Some suggest that both populations can restore the tracheal epithelium equally well (Avril-Delplanque et al, 2005;Liu et al, 1994). However, others have found that only columnar cells (Johnson and Hubbs, 1990) or only basal cells (Ford and Terzaghi-Howe, 1992) can restore all of the epithelial cell types. These discrepancies may be due to differences in sorting methods, donor species or the length of time allowed for epithelial repopulation.…”

Section: Box 2 Transdifferentiationmentioning

confidence: 99%

Epithelial stem cells of the lung: privileged few or opportunities for many?

2006

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Most reviews of adult stem cells focus on the relatively undifferentiated cells dedicated to the renewal of rapidly proliferating tissues, such as the skin, gut and blood. By contrast, there is mounting evidence that organs and tissues such as the liver and pancreatic islets, which turn over more slowly,use alternative strategies, including the self-renewal of differentiated cells. The response of these organs to injury may also reveal the potential of differentiated cells to act as stem cells. The lung shows both slow turnover and rapid repair. New experimental approaches, including those based on studies of embryonic development, are needed to identify putative lung stem cells and strategies of lung homeostasis and repair.

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Section: Box 2 Transdifferentiationmentioning

confidence: 99%

Epithelial stem cells of the lung: privileged few or opportunities for many?

2006

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“…Several studies suggest that basal cells are progenitors (28)(29)(30)(31)(32), but a strong proliferative response in secretory cells following injury (33), the late developmental appearance of basal cells (34)(35)(36), and studies with isolated cell populations (37) make a case for small-granule secretory cells or an undifferentiated columnar cell. There is evidence for great plasticity in growth and differentiation potential of airway epithelial cells (38) and our earlier studies showing that both basal and non- basal cells could regenerate a complete mucociliary epithelium in tracheal grafts support this notion (16,39).…”

Section: Are Airway Epithelial Stem Cells Present In Distinct Morpholmentioning

confidence: 99%

Evidence for Stem-Cell Niches in the Tracheal Epithelium

Borthwick

Shahbazian

Krantz

et al. 2001

Am J Respir Cell Mol Biol

378

335

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It is generally important to elucidate airway epithelial cell lineages and to identify multipotent progenitors as targets for gene therapy. Stem (S) cells are typically present in specialized compartments spatially proximal to their differentiated progeny, but an equivalent paradigm has not been demonstrated in the airway. We discovered a distinct population of cells displaying high levels of keratin expression in murine tracheal submucosal gland ducts, and tested the hypothesis that bromodeoxyuridine (BrdU) label-retaining cells (LRCs), thought to represent the S-cells, were present in this compartment. Mice received weekly epithelial damage by intratracheal detergent or SO(2) inhalation for 4 wk and received intraperitoneal injections of BrdU every 48 h during the injury and repair period. At 3 and 6 d after injury, BrdU-positive epithelial cells were noted along the entire tracheal length in both basal and lumenal cell positions. At later time points (20 and 95 d) LRCs were localized to gland ducts in the upper trachea and to systematically arrayed foci in the lower trachea, typically near the cartilage-intercartilage junction. LRCs were not pulmonary neuroendocrine cells. Heterotopic tracheal grafts after surface epithelial removal demonstrated reconstitution of a surface-like epithelium from gland remnants. These results suggest that airway epithelial S cells are localized to specific niches.

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“…Several approaches have been developed to identify progenitor cells in the normal or injured airway epithelium such as 3 H-thymidine radiolabeling [8,9], in vitro or in vivo development of sorted subsets of cells [10][11][12][13][14][15][16][17][18][19], and cell-lineage tracking with recombinant retroviruses [20][21][22]. Studies of cell turnover in the normal respiratory epithelium designate basal cells as potential stem cells [23].…”

Section: Introductionmentioning

confidence: 99%

“…However, the strong proliferation of secretory cells after chemical or physical injury [5,7] suggests that these may also represent candidate progenitor cells. Cells constituting the airway mucosa have been separated on differences in density [10][11][12]15], or by flow cytometry on the basis of cell size [13,14,16] or surface-antigen expression [17][18][19]. This material is protected by U.S.…”

Section: Introductionmentioning

confidence: 99%

Aquaporin‐3 Expression in Human Fetal Airway Epithelial Progenitor Cells

et al. 2005

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Airway epithelium stem cells have not yet been prospectively identified, but it is generally assumed that both secretory and basal cells have the capacity to divide and differentiate. Previously, we developed a test for progenitor cells of the human airway epithelium, relying on the transplantation of fetal respiratory tissues into immunodeficient mice. In this study, we hypothesized that airway-repopulating epithelial progenitors can be marked with surface antigens, and we screened an array of such candidate markers, including lectin ligands, the CD44 and CD166 adhesion molecules, and the aquaporin-3 (AQP3) water channel. We observed that AQP3 is selectively expressed on the surface of basal cells, allowing the separation by flow cytometry of AQP3 + basal cells and AQP3 -ciliated and secretory cells. Functional evaluation of sorted cells in vivo showed that AQP3 + cells can restore a normal pseudostratified, mucociliary epithelium as well as submucosal glands. AQP3 -cells are also endowed with a similar potential, although faster engraftment suggests their inclusion of more committed progenitors. These results show that stem cell candidates in the human tracheo-bronchial mucosa can be positively selected with a novel marker but also, for the first time, that epithelial progenitors exist among both basal and suprabasal cell subsets within the human airway. Stem Cells 2005;23:992-1001

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Characteristics of magnetically separated rat tracheal epithelial cell populations

Cited by 8 publications

References 0 publications

Epithelial stem cells of the lung: privileged few or opportunities for many?

Epithelial stem cells of the lung: privileged few or opportunities for many?

Evidence for Stem-Cell Niches in the Tracheal Epithelium

Aquaporin‐3 Expression in Human Fetal Airway Epithelial Progenitor Cells

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