1983
DOI: 10.1128/jb.155.2.878-885.1983
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Characteristics of major outer membrane proteins of Haemophilus influenzae

Abstract: Several properties of Haemophilus influenzae outer membrane proteins were analyzed to define related proteins in various isolates. H. influenzae type b 760705 had six major outer membrane proteins with the following characteristics. Protein a (Mr, 47,000) demonstrated heat modifiability in sodium dodecyl sulfate; its apparent molecular weight was 34,000 at temperatures below 60°C. This protein was extracted from cell envelopes by using Triton X-100-10 mM MgCl2; in cell envelope preparations, the protein was de… Show more

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Cited by 99 publications
(57 citation statements)
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“…This approach opens up the possibility of preventing serious H.influenzae infections, including meningitis, epiglottitis, recurrent sinusitus and otitis media, and chronic bronchitis, by interfering with the interaction between the receptor site and the bacteria, thus preventing its adherence to and colonisation 71 of the mucosa. This interaction seems to be common for various H.influenzae strains, despite the strongly varying antigenic composition of their surface-exposed components [7].…”
Section: Discussionmentioning
confidence: 96%
“…This approach opens up the possibility of preventing serious H.influenzae infections, including meningitis, epiglottitis, recurrent sinusitus and otitis media, and chronic bronchitis, by interfering with the interaction between the receptor site and the bacteria, thus preventing its adherence to and colonisation 71 of the mucosa. This interaction seems to be common for various H.influenzae strains, despite the strongly varying antigenic composition of their surface-exposed components [7].…”
Section: Discussionmentioning
confidence: 96%
“…It shows that the killing-promoting antibodies were removed by absorption with purified protein a, with cells of the homologous strain 760705, but not with purified lipopolysaccharide, polysaccharide or cells of strain 1481. This strain has a protein a with a different molecular mass and immunological properties than strain 760705 ( [5], L. van Alphen Table 2 Relation between titres of antibodies against protein a, lipopolysaccharide and polysaccharide of variously absorbed anti-a sera and killing of strain 760705b ° et al, Submitted for publication). Reduction in killing coincided with a strong reduction in antiprotein a titre and not with reduction in anti-lipopolysaccharide.…”
Section: Resultsmentioning
confidence: 99%
“…The H. influenzae type b strains used throughout this study were isolated from the CSF of patients with meningitis. Their (sub) typing characteristics have been described before [4,5] and are summarised in Table 1. Strain 1481 was a non-typable strain isolated from sputum of a patient with a respiratory tract infection.…”
Section: Bacterial Strains and Growth Conditionsmentioning
confidence: 99%
See 1 more Smart Citation
“…H. in£uenzae was identi¢ed by its dependence for growth factors X and V and its inability to convert Naminolevulanic acid to porphyrins [19]. The strains were characterized phenotypically by major outer membrane protein (MOMP) subtyping as described previously [20]. In addition, genotypic characterization was performed by randomly ampli¢ed polymorphic DNA (RADP) analysis with the primers ERIC1 and ERIC2 as described before [21].…”
Section: Characteristics Of Patients and Bacterial Strainsmentioning
confidence: 99%