Characteristics of Mel Ibr Melanoma Line Subclone after Treatment with Chicken Embryo Extract

Сураева, Н. М.; Морозова, Л. Ф.; Ryabaya, O. O.; Khochenkova, Yu. A.; Самойлов, А. В.; Бурова, О. С.; Golubtsova, N. V.; Бармашов, А. Е.; Барышникова, М А

doi:10.1007/s10517-017-3778-y

Cited by 3 publications

(5 citation statements)

References 12 publications

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“…Низкий уровень CD271 в исходной линии не обнаруживался в субклоне. Роль антигена CD271 в прогрессии меланомы CD271 остается спорной [29].…”

Section: субклон Mel Ibr Eeunclassified

“…Это свидетельствует о пригодности субклона для получения солидной опухоли у мышей Balb/c nude из культуры клеток уже на «нулевом» пассаже in vivo. Полученная модель КЛ mel Ibr EE/BRAF + адекватна in vitro для оценки антипролиферативной активности и эффективности многократной таргетной или цитостатической те рапии in vivo, направленной на клетки с мутацией BRAF V600E [29].…”

Section: субклон Mel Ibr Eeunclassified

“…По морфологическим характеристикам клетки субклона mel Ibr 5С близки к субклону mel Ibr EE и отличаются от родительских клеток значительно меньшим размером, крупными ядрами, занимающими практически весь объем цитоплазмы, длинными вытянутыми отростками и интенсивной пролиферацией [29,30]. Эти клетки растут в виде плотного монослоя и формируют на его поверхности выпуклые образования в виде сфероидов.…”

Section: субклон Mel Ibr 5сunclassified

“…Экспрессия антигена CD63 в клетках субклона повысилась, а экспрессия антигенов гистосовместимости I класса: HLA-ABC, CD24, CD34, CD44, CD117 и CD105 не изменилась (табл. 2) [29,30].…”

Section: субклон Mel Ibr 5сunclassified

“…Цитогенетический анализ показал, что кариотип клеток субклона mel Ibr 5К и родоначальной КЛ различен [24,29,30].…”

Section: субклон Mel Ibr 5сunclassified

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Cell lines of human melanoma and their xenograft with braf or nras mutations a targets for targeted therapy. Reviews

Михайлова

Трещалина

Морозова

et al. 2019

Rossijskij bioterapevtičeskij žurnal

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The review presents a discussion on articles and patents, describing new in vitro and in vivo models of pigmented or non-pigmented human cutaneous melanoma, received in NMRCO from the patients» metastases. Molecular genetic characteristics of the new models is supported by the arguments in addition to the given data and visual materials. The subjects of the discussed publications are 3 polyclonal cell lines, 2 subclones and 4 subcutaneous (s/c) xenografts in immunodeficient mice Balb/c nude. All the models are stored in Cryo Collection with xenografts at N.N. Blokhin NMRCO as well as in the Russian Collection of Cell Cultures of Vertebrae (RCCCV, St. Petersburg). This mini-collection is recommended for use in basic research of cutaneous melanoma and pre-clinical studies of anti-melanoma agents. The basis for these studies are the appropriate characteristics of the models, including cytological, immunologic, transplantation and molecular-genetic ones, as well as in vivo drug sensitivity to the corresponding target therapy.

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Section: субклон Mel Ibr Eeunclassified

Section: субклон Mel Ibr 5сunclassified

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Cell lines of human melanoma and their xenograft with braf or nras mutations a targets for targeted therapy. Reviews

Михайлова

Трещалина

Морозова

et al. 2019

Rossijskij bioterapevtičeskij žurnal

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Conservation of Epithelial-to-Mesenchymal Transition Process in Neural Crest Cells and Metastatic Cancer

Zhang

Aslam

Sharma

et al. 2021

Cells Tissues Organs

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Epithelial to mesenchymal transition (EMT) is a highly conserved cellular process in several species, from worms to humans. EMT plays a fundamental role in early embryogenesis, wound healing, and cancer metastasis. For neural crest cell (NCC) development, EMT typically results in forming a migratory and potent cell population that generates a wide variety of cell and tissue, including cartilage, bone, connective tissue, endocrine cells, neurons, and glia amongst many others. The degree of conservation between the signaling pathways that regulate EMT during development and metastatic cancer (MC) has not been fully established, despite ample studies. This systematic review and meta-analysis dissects the major signaling pathways involved in EMT of NCC development and MC to unravel the similarities and differences. While the FGF, TGFβ/BMP, SHH, and NOTCH pathways have been rigorously investigated in both systems, the EGF, IGF, HIPPO, Factor Receptor Superfamily, and their intracellular signaling cascades need to be the focus of future NCC studies. In general, meta-analyses of the associated signaling pathways show a significant number of overlapping genes (particularly ligands, transcription regulators, and targeted cadherins) involved in each signaling pathway of both systems without stratification by body segments and cancer type. Lack of stratification makes it difficult to meaningfully evaluate the intracellular downstream effectors of each signaling pathway. Finally, pediatric neuroblastoma and melanoma are NCC-derived malignancies, which emphasize the importance of uncovering the EMT events that convert NCC into treatment-resistant malignant cells.

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Directing Rat Hair Follicle Stem Cells Toward Neuronal Lineage With Enhanced Trophic Factor Expression

Pandamooz,

Chavoshinezhad,

Mostaghel

et al. 2024

Advanced Biomedical Research

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Background: Hair follicle stem cells (HFSCs) are promising candidates for cell-based therapies in neurodegenerative diseases because of their ability to differentiate into neural lineages and exert paracrine effects in damaged tissues. However, their clinical application faces challenges, particularly in efficiently guiding them toward neural lineages. This study explores using chick embryo extract (CEE) to enhance HFSCs’ secretory capacity and neuronal differentiation. Materials and Methods: HFSCs from rat whisker pads were cultured in growth medium supplemented with either 20% FBS or a combination of 10% FBS and 10% CEE, transitioning to 20% FBS after the first subculture. We conducted gene expression profiling of lineage commitment markers and neurotrophic factors in both experimental groups, alongside morphological assessments and protein expression analyses. Results: CEE supplementation during migration increased neuronal differentiation, evidenced by more cells with neurites and higher MAP2 expression at both the gene and protein levels. CEE also inhibited the expression of PDGFR-α, indicating a suppression of differentiation toward Schwann cells. Furthermore, we observed increased levels of trophic factors such as BDNF and VEGF at passage 3 induced by CEE supplementation. Conclusions: Enhancing the neuronal lineage commitment of hair follicle stem cells (HFSCs) and boosting the expression of trophic and angiogenic factors through short-term CEE preconditioning during their migratory stage presents a compelling approach. This strategy holds great promise in enhancing the effectiveness of stem cell-based therapies for neurological disorders.

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Characteristics of Mel Ibr Melanoma Line Subclone after Treatment with Chicken Embryo Extract

Cited by 3 publications

References 12 publications

Cell lines of human melanoma and their xenograft with braf or nras mutations a targets for targeted therapy. Reviews

Cell lines of human melanoma and their xenograft with braf or nras mutations a targets for targeted therapy. Reviews

Conservation of Epithelial-to-Mesenchymal Transition Process in Neural Crest Cells and Metastatic Cancer

Directing Rat Hair Follicle Stem Cells Toward Neuronal Lineage With Enhanced Trophic Factor Expression

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