Characteristics of multiresistant Pseudomonas aeruginosa isolates from burn patients in Iran

Abednezhad, Arash; Bakhshi, Bita; Moghadam, Nastaran Asghari; Faraji, Nima; Derakhshan-Nezhad, Elahe; Barzelighi, Hajar Mohammadi

doi:10.1556/030.2022.01882

Cited by 4 publications

(2 citation statements)

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“…Among the diversity of STs found in this work, the high-risk clone ST235 was encountered in two strains. ST235 has been largely reported in different other infections such as in wound infections and in burn patients [70–72] and has been associated with fatal patient outcomes [33, 73–76]. However, to the best of our knowledge, ST235 has not been previously described in DFIs, highlighting the presence and subsequent dissemination of this clone in this type of infections.…”

Section: Discussionmentioning

confidence: 94%

Molecular characterization of Pseudomonas aeruginosa from diabetic foot infections in Tunisia

Arfaoui,

Rojo-Bezares,

Fethi

et al. 2024

Journal of Medical Microbiology

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Background. Pseudomonas aeruginosa is an invasive organism that frequently causes severe tissue damage in diabetic foot ulcers. Gap statement. The characterisation of P. aeruginosa strains isolated from diabetic foot infections has not been carried out in Tunisia. Purpose. The aim was to determine the prevalence of P. aeruginosa isolated from patients with diabetic foot infections (DFIs) in Tunisia and to characterize their resistance, virulence and molecular typing. Methods. Patients with DFIs admitted to the diabetes department of the International Hospital Centre of Tunisia, from September 2019 to April 2021, were included in this prospective study. P. aeruginosa were obtained from the wound swabs, aspiration and soft tissue biopsies during routine clinical care and were confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Antimicrobial susceptibility testing, serotyping, integron and OprD characterization, virulence, biofilm production, pigment quantification, elastase activity and molecular typing were analysed in all recovered P. aeruginosa isolates by phenotypic tests, specific PCRs, sequencing, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. Results. Sixteen P. aeruginosa isolates (16.3 %) were recovered from 98 samples of 78 diabetic patients and were classified into 6 serotypes (O:11 the most frequent), 11 different PFGE patterns and 10 sequence types (three of them new ones). The high-risk clone ST235 was found in two isolates. The highest resistance percentages were observed to netilmicin (69 %) and cefepime (43.8 %). Four multidrug-resistant (MDR) isolates (25 %) were detected, three of them being carbapenem-resistant. The ST235-MDR strain harboured the In51 class 1 integron (intI1 +aadA6+orfD+qacED1-sul1). According to the detection of 14 genes involved in virulence or quorum sensing, 5 virulotypes were observed, including 5 exoU-positive, 9 exoS-positive and 2 exoU/exoS-positive strains. The lasR gene was truncated by ISPpu21 insertion sequence in one isolate, and a deletion of 64 bp in the rhlR gene was detected in the ST235-MDR strain. Low biofilm, pyoverdine and elastase production were detected in all P. aeruginosa; however, the lasR-truncated strain showed a chronic infection phenotype characterized by loss of serotype-specific antigenicity, high production of phenazines and high biofilm formation. Conclusions. Our study demonstrated for the first time the prevalence and the molecular characterization of P. aeruginosa strains from DFIs in Tunisia, showing a high genetic diversity, moderate antimicrobial resistance, but a high number of virulence-related traits, highlighting their pathological importance.

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Section: Discussionmentioning

confidence: 94%

Molecular characterization of Pseudomonas aeruginosa from diabetic foot infections in Tunisia

Arfaoui,

Rojo-Bezares,

Fethi

et al. 2024

Journal of Medical Microbiology

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“…Ma et al demonstrated that the psl gene plays a critical role in adherence of cell surfaces [3]. Bioflm production by P. aeruginosa facilitates its persistence in the environment and can lead to severe bacterial infections, especially in hospitalized patients [4]. However, in vitro bioflm production among clinical strains varies, and P. aeruginosa can be classifed as strong, moderate, weak, or nonbioflm producers [5,6].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Aspects of Antibiotic Resistance and Biofilm Formation of P. aeruginosa Isolated from Clinical Samples

Motevasel,

Haghkhah,

Azimzadeh

2024

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Introduction. Biofilm production and drug resistance phenomenon play a critical role in P. aeruginosa infections. Several genes, including psl, pel, brlR, and mex, are involved in the phenomenon. The aim of this study was to find the relationship between the mentioned genes and the sources of P. aeruginosa infections. Materials and Methods. Fifty-nine P. aeruginosa isolates detected from clinical specimens were used to determine antibiotic susceptibility patterns, prevalence of the genes using PCR, biofilm formation, biofilm eradication concentration assay (MBEC), and epidemiological characteristics using pulsed-field gel electrophoresis (PFGE). Results. The results showed that 35.6% and 16.94% of all the samples were isolated from urine and wounds, 81.33% of the isolates were biofilm producers, 27.11% were multidrug-resistant (MDR), and 100% of the main biofilm former genes belonged to pslA. 94.91% of the isolates possessed brlR and mexA, and 91.5% of them expressed pslA. It was also indicated that neither ciprofloxacin nor imipenem could eradicate the formed biofilms. Moreover, we could identify 81.4% distinctive restriction profiles among the isolates, using an 80% similarity cutoff point; brlR and pel genes were significantly (P=0.032; P=0.044) related to phylogenetic pulsotypes. Comparison of the dendrogram in the isolates revealed that the detected isolates from urine were present in 12 different pulsotypes. Conclusion. It was found that there was a relationship between MDR, biofilm production, and brlR and pel genes among the isolates. It is distinguished there were similar genetic patterns between detected isolates from urine and could be concluded that the urinary tract played a critical role in maintaining and transferring biofilm drug-resistant genes of P. aeruginosa in clinical sites. The study highlights the importance of urine in distribution of clinical biofilm formation and drug-resistant P. aeruginosa isolates.

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The frequency of AmpC overproduction, OprD downregulation and OprM efflux pump expression in Pseudomonas aeruginosa: A comprehensive meta-analysis

Alimoghadam,

Eslami,

Alimoghadam

et al. 2024

Journal of Global Antimicrobial Resistance

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Characteristics of multiresistant Pseudomonas aeruginosa isolates from burn patients in Iran

Cited by 4 publications

References 0 publications

Molecular characterization of Pseudomonas aeruginosa from diabetic foot infections in Tunisia

Molecular characterization of Pseudomonas aeruginosa from diabetic foot infections in Tunisia

Phylogenetic Aspects of Antibiotic Resistance and Biofilm Formation of P. aeruginosa Isolated from Clinical Samples

The frequency of AmpC overproduction, OprD downregulation and OprM efflux pump expression in Pseudomonas aeruginosa: A comprehensive meta-analysis

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