Characteristics of muscle fibers reconstituted in the regeneration process of masseter muscle in an mdx mouse model of muscular dystrophy

Lee, Won Hyung; Abe, Shinichi; Kim, Hee Jin; Usami, Akinobu; Honda, A; Sakiyama, Kazuo; Ide, Yoshinobu

doi:10.1007/s10974-006-9066-5

Cited by 24 publications

(22 citation statements)

References 27 publications

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“…It was reported that, in somite-derived muscles of the extremities, muscle necrosis began at 2 weeks after birth, immediately followed by regeneration, and the majority of necrotic muscles became regenerated at 4 weeks after birth (Dangain et al 1984;DiMario et al 1991;Attal et al 2000). A similar phenomenon was noted in the branchial arch-derived mdx mouse masseter muscle (Lee et al 2006), and cell degeneration in this muscular dystrophy was ascribed to the lack of dystrophin, resulting in damage to cell membrane stability, leading to excessive influx of calcium ions into muscle cells, a mechanism similar to that of necrosis.…”

Section: Introductionmentioning

confidence: 58%

Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis

Honda

Abe

Hiroki

et al. 2007

J Muscle Res Cell Motil

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The mdx mouse, a model of muscular dystrophy, lacks dystrophin, a cell membrane protein. It is known that the lack of dystrophin causes muscle fiber necrosis from 2 weeks after birth, and the majority of necrotic muscle fibers are replaced by regenerated muscle fibers by 4 weeks after birth. A recent study indicated the possibility that mitochondria-mediated intracellular stress, a phenomenon similar to apoptosis, may be produced during muscle fiber necrosis, but did not analyze endoplasmic reticulum-mediated intracellular stress.Therefore, we examined the expression of the caspase-12 gene involved in the endoplasmic reticulum stress pathway and the Bax, caspase-9, and caspase-3 genes involved in the mitochondrial stress pathway in the mdx masseter muscle. We found over-expression of caspase-12 in cells at 2-3 weeks after birth when muscle fiber necrosis was not prominent. This suggests that s tress occurs in the endoplasmic reticulum to maintain cell morphology in the absence of dystrophin. In addition, Bax was abundantly expressed in the mdx masseter muscle at 3 weeks after birth, and the expression of caspase-9 and -3 was prominent at 3-4 weeks after birth when necrosis and regeneration were marked. These results indicate that endoplasmic reticulum and mitochondrial stresses are produced during necrosis of 3 the mdx masseter muscle, and suggest that these events are a phenomenon similar to apoptosis.

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Section: Introductionmentioning

confidence: 58%

Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis

Honda

Abe

Hiroki

et al. 2007

J Muscle Res Cell Motil

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“…Muscle proteins, which contribute to the structure of myoblasts, normally show turnover through new synthesis and degradation (Okubo et al, 2006;Lee et al, 2006;Suzuki et al, 2007). Any imbalance between synthesis and degradation results in an increase or decrease in muscle volume; for example, promotion of the synthesis pathway or inhibition of the degradation pathway in myoblasts will cause an increase in muscle proteins.…”

Section: Discussionmentioning

confidence: 99%

Effects of Mechanical Stretching on Caspase and IGF-1 Expression During the Proliferation Process of Myoblasts

et al. 2008

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It has been reported that the synthesis, degradation, and metabolism of muscle proteins in myoblasts, as well as the proliferation and differentiation of cells, are influenced by various related to extracellular signaling molecules, such as neural transmitters, growth factors, and hormones, when muscle tissue has been exposed to mechanical stimulation. However, reports regarding the expression of growth factors during mechanical stimulation of myoblasts are few, and many questions remain unanswered. We examined the mRNA expression of insulin-like growth factor 1 (IGF-1) in myoblasts subjected to mechanical stretching in vitro. In addition, apoptosis caused by intracellular stress has been reported to occur during muscle development at the embryonic stage. To clarify the expression of intracellular stress factors, we here investigated related gene expression. Expression of IGF-1 increased in the early stage of cell stretching, followed by a decrease in the late stage. This suggests that mechanical stimulation resulted in an immediate increase in IGF-1 expression, followed by a decrease as cells acclimated to the inducing environment. Caspase was significantly expressed in a stretch group at 12 hours after the beginning of mechanical stimulation, compared with a control group. This suggests that cellular proliferation is also regulated by intracellular stress factors involving the endoplasmic reticulum, mitochondria, and other organelles during the process of muscle proliferation and differentiation

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“…It was reported that, in somite-derived muscles of the extremities, muscle necrosis began at 2-week after birth, immediately followed by regeneration, and the majority of necrotic muscles became regenerated at 4-week after birth (Dangain et al, 1984;DiMario et al, 1991;Attal et al, 2000). A similar phenomenon was noted in the branchial arch-derived mdx mouse masseter muscle (Lee et al 2006), and cell degeneration in this muscular dystrophy was ascribed to the lack of dystrophin, resulting in damage to cell membrane stability, leading to excessive influx of calcium ions into muscle cells, a mechanism similar to that of necrosis. Thus, mdx mice have been used as a model to study the mechanism of muscle necrosis and regeneration (Gillis, 1996).…”

Section: Discussionmentioning

confidence: 55%

Increased expression of decorin during the regeneration stage of mdx mouse

et al. 2009

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Thus, decorin appeared as a new molecule in myostatin signaling pathway and arose as a promising target for treatment of progressive neuromuscular diseases. Therefore in the current study, we examined localization of decorin as well as myostatin in a muscle dystrophy model mdx mice and B10 Scott Snells mice as a control to elucidate the differences of decorin and myostatin message as well as protein distribution. The current study revealed increased expression of decorin protein as well as mRNA at the regenerative stage of mdx mouse as compared to early stages, while only weak expression of decorin was detected at the control mice. Our study would contribute to 3 identify the relationship between decorin and myostatin as well as development of therapeutic strategy for progressive neuromuscular diseases.

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Characteristics of muscle fibers reconstituted in the regeneration process of masseter muscle in an mdx mouse model of muscular dystrophy

Cited by 24 publications

References 27 publications

Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis

Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis

Effects of Mechanical Stretching on Caspase and IGF-1 Expression During the Proliferation Process of Myoblasts

Increased expression of decorin during the regeneration stage of mdx mouse

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