Characteristics of n-octyl <i>β</i>-<scp>d</scp>-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry

Saito, Shuntaro; Tsuchiya, Tomofusa

doi:10.1042/bj2220829

Cited by 64 publications

(34 citation statements)

References 15 publications

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“…A crude soybean phosphatidylcholine preparation (actual content, 14%) containing many other lipids stimulated NO reductase activity threefold, but purified phosphatidylcholine showed no effect. An increase in activity of the same magnitude as with soybean lipid was observed on adding n-octyl-,B-D-glucopyranoside or its thio derivative (42) ence of 0.33% n-octyl-,-D-thioglucopyranoside. Calculations were based on an NO solubility of 5.17 mg/100 g of water at 30°C (28).…”

Section: Resultssupporting

confidence: 51%

Formation of the N-N bond from nitric oxide by a membrane-bound cytochrome bc complex of nitrate-respiring (denitrifying) Pseudomonas stutzeri

1989

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Section: Resultssupporting

confidence: 51%

Formation of the N-N bond from nitric oxide by a membrane-bound cytochrome bc complex of nitrate-respiring (denitrifying) Pseudomonas stutzeri

1989

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“…These properties are very appreciated for the purpose of solubilization of the membrane of proteins, a field in which these surfactants are extensively used. 4 In addition, the increased popularity of these products is motivated by the fact that they are nontoxic, biodegradable, and considered dermatologically safe. Therefore, these surfactants find extensive applications as liquid dishwashing agent, detergents, and personal care products, as well as hard surface cleaners, agrochemical, and for industrial and institutional cleaning.…”

Section: Introductionmentioning

confidence: 99%

“…4 Moreover, recent investigations have pointed out the advantages of OTG against OG in several applications in the biomembrane field. 4,21,22 In many of the applications mentioned above, it is frequently the presence of electrolyte in the medium, and these substances may induce changes in both intramicellar and intermicellar interactions. 23 It is well-known that, in the case of nonionic surfactants, these interactions are relatively weak; however, they can modify both structural and dynamic properties of micelles, determining the behavior of the surfactant in a certain application.…”

Section: Introductionmentioning

confidence: 99%

Effect of NaCl on the Self-Aggregation of n-Octyl β-d-Thioglucopyranoside in Aqueous Medium

2006

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This report investigates the effect of sodium chloride (NaCl) on the micellization, surface activity, and the evolution in the shape and size of n-octyl -D-thioglucopyranoside (OTG) aggregates. By using surface tension measurements, information was obtained on both changes in the critical micelle concentration and adsorption behavior in the air-liquid interface with the electrolyte concentration. These data were used to obtain the thermodynamic properties of micellization along with the corresponding adsorption parameters in the airliquid interface. From extended static and dynamic light scattering measurements, the micelle molecular weight, the mean aggregation number, and the second virial coefficient, the apparent diffusion coefficient and the mean hydrodynamic radius of micelles in a range of NaCl concentrations were obtained. The light scattering data have shown that when the surfactant concentration is lower to 4.5 g/L, only spherical micelles are formed. However, an increase in the surfactant concentration induces an increase in micellar size, suggesting a rodlike growth of the micelles. This deviation of micelle geometry from spherical to rodlike is supported both by the ratio between the hydrodynamic radius and the radius of gyration and by the angular dependence of light scattering. On the other hand, the studies performed in the presence of high NaCl concentration (0.2 and 0.5 M) provide strong support for the view that the micelles may overlap together to form an entangled network above certain crossover concentration.

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“…The use of both detergents solubilized about 32% of the membrane proteins and yielded similar electrophoretic protein patterns and transporter reconstitution. Octylthioglucoside, however, has a greater pH stability (Saito and Tsuchiya, 1984) which might be advantageous for future purification steps involving pH gradients. Subsequent removal of the detergent by dialysis and ultrafiltration resulted in a yield of about 0.8% of total ghost protein or 0.2-0.3% of total cell protein.…”

Section: Enrichment Of the D-glucose Transportermentioning

confidence: 99%

Functional reconstitution of the Trypanosoma brucei plasma‐membrane D‐glucose transporter

Seyfang

Duszenko

1993

European Journal of Biochemistry

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The D-glucose transporter of Trypanosoma brucei was solubilized from the plasma membrane and reconstituted into proteoliposomes. Using the reconstitution of D-glucose transport as the assay and non-specific L-glucose uptake as control, we have purified a membrane protein fraction from 7: brucei bloodstream-form ghosts by EDTMalkali treatment and solubilization with the detergents octylglucoside or octylthioglucoside. Upon removal of the detergent by dialysis, the solubilized protein fraction was reconstituted in sonicated liposomes by a freezehaw-sonication step. The reconstituted transporter catalyzed specific D-glucose uptake and was compared in several characteristics with the native facilitated-diffusion transporter as present in live trypanosomes [Seyfang, A. & Duszenko, M. (1991) Eul: J. Biochem. 202, 191-1961. As in vivo, the uptake is time dependent and Na' independent. Transporter substrate affinity and inhibitor specificity are completely retained and it is inhibited by mercuric ions, phloretin and cytochalasin B, but only partially inhibited by phlorizin. The reconstituted transporter also demonstrates trans-stimulation properties indicative of the carrier-mediated transport of D-glucose. In contrast to the human erythrocyte-type glucose transporter, in Z brucei D-fructose uptake was also catalyzed by the same reconstituted protein fraction and specific D-glucose or D-fructose transport were mutually competitive. Both the inhibitor studies and the fructose transport capacity in the reconstituted system are in good agreement with the native transport in live trypanosomes. The specific activity of D-glucose transport was 1.9 5 0.3 nmolmin-'. mg protein-' at 0.2 mM D-glucose and the yield was about 0.8% of total ghost protein after removal of the variant-surface-glycoprotein coat. The successful functional reconstitution of a protozoan glucose transporter represents an important step towards its purification and detailed characterization. This is especially interesting since bloodstream-form trypanosomes depend entirely upon glycolysis for their ATP production.

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Characteristics of n-octyl β-d-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry

Cited by 64 publications

References 15 publications

Formation of the N-N bond from nitric oxide by a membrane-bound cytochrome bc complex of nitrate-respiring (denitrifying) Pseudomonas stutzeri

Formation of the N-N bond from nitric oxide by a membrane-bound cytochrome bc complex of nitrate-respiring (denitrifying) Pseudomonas stutzeri

Effect of NaCl on the Self-Aggregation of n-Octyl β-d-Thioglucopyranoside in Aqueous Medium

Functional reconstitution of the Trypanosoma brucei plasma‐membrane D‐glucose transporter

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