Characteristics of Neisseria gonorrhoeae Strains Isolated on Selective and Nonselective Media

Pace, Paul J.; Catlin, B. Wesley

doi:10.1097/00007435-198601000-00007

Cited by 9 publications

(6 citation statements)

References 25 publications

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“…This did not correlate with the fact that gonococci obviously have a thyA gene. Pace and Catlin [12] reported that gonococci are resistant to trimethoprim at levels ≥5 μg ml −1 , a concentration sufficiently toxic to E. coli on minimal medium [6]. However, E. coli strain KLC1597 is resistant to trimethoprim at levels of 50 μg ml −1 on rich medium (this study).…”

Section: Resultsmentioning

confidence: 66%

“…Pace and Catlin [12] reported that most N. gonorrhoeae strains are naturally resistant to trimethoprim. This did not correlate with the fact that gonococci obviously have a thyA gene.…”

Section: Resultsmentioning

confidence: 99%

“…Unlike E. coli, Neisseria gonorrhoeae lacks the salvage enzymes required for conversion of thymine and thymidine to dTMP [8]. Furthermore, Pace and Catlin [12] reported that the majority of the gonococcal strains they tested were naturally resistant to trimethoprim, suggesting that gonococci also lack thymidylate synthase. If so, the gonococcus either synthesizes dTMP in a unique, heretofore undescribed manner or salvages dTMP via an alternative pathway.…”

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae

Carlson

Hill

2006

FEMS Microbiology Letters

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Thymidylate synthase converts deoxyuridylate to deoxythymidylate. The thyA gene has been cloned and sequenced from Neisseria gonorrhoeae MS11. This gene has an open reading frame of 795-801 bp and encodes a product which shares 71% identity with its Escherichia coli homolog. Unlike its E. coli counterpart, gonococcal thyA has a large, upstream transcribed region (300+ bp) that lacks a translatable reading frame. Gonococcal thyA is capable of complementing an E. coli thyA null mutant and shares similar levels of sensitivity with trimethoprim.

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Section: Resultsmentioning

confidence: 66%

“…Pace and Catlin [12] reported that most N. gonorrhoeae strains are naturally resistant to trimethoprim. This did not correlate with the fact that gonococci obviously have a thyA gene.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae

Carlson

Hill

2006

FEMS Microbiology Letters

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“…AHU strains were uniformly hypersusceptible to vancomycin (Exner et al 1982; Pace and Catlin 1986), suggesting that a mutation of an envelope locus may be responsible. This could mean a number of things are occurring within these strains.…”

Section: Resultsmentioning

confidence: 99%

Differences in nitric oxide steady states between arginine, hypoxanthine, uracil auxotrophs (AHU) and non-AHU strains ofNeisseria gonorrhoeaeduring anaerobic respiration in the presence of nitrite

Barth

Clark

2008

Can. J. Microbiol.

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Neisseria gonorrhoeae can grow by anaerobic respiration using nitrite as an alternative electron acceptor. Under these growth conditions, N. gonorrhoeae produces and degrades nitric oxide (NO), an important host defense molecule. Laboratory strain F62 has been shown to establish and maintain a NO steady-state level that is a function of the nitrite reductase/nitric oxide reductase ratio and is independent of cell number. The nitrite reductase activities (122-197 nmoles NO 2 reduced/ min-OD 600 ) and nitric oxide reductase activities (88-155 nmoles NO reduced/ min-OD 600 ) in a variety of gonococcal clinical isolates were similar to the specific activities seen in F62 (241 nmoles NO 2 reduced/ min-OD and 88 nmoles NO reduced/ min-OD, respectively). In 7 gonococcal strains, the NO steady state levels established in the presence of nitrite were similar to that of F62 (801-2121 nM NO), while 6 of the strains, identified as arginine, hypoxanthine, and uracil auxotrophs (AHU), that cause asymptomatic infection in men, had either a 2-to 3-fold (373-579 nM NO) or about 100-fold (13-24 nM NO) lower NO steady state concentrations. All tested strains in the presence of a NO-donor, DETA/NO, quickly lowered and maintained NO levels in the non-inflammatory range of NO (<300 nM). The generation of a NO steady-state concentration was directly affected by alterations in respiratory control in both F62 and an AHU strain, although differences in membrane function are suspected to be responsible for NO steady-state level differences in AHU strains.

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“…Selbst wenn optimale Voraussetzungen für die mikrobiologische Diagnostik gegeben sind, kann die Sensitivität der Kulturmethode durch Antibiotikaeinnahme oder durch lokale Desinfektionsmittel beeinträchtigt werden. Ebenso können in Einzelfällen Vancomycin-empfindliche Gonokokkenstämme (11) vorkommen, die auf Thayer-Martin Agar kein Wachstum zeigen. Diese Gonokokkenstämme könnten von der Begleitflora auf der mitgeführten Kochblutplatte überwuchert werden und damit dem Nachweis entgehen.…”

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DNA-Hybridisierung zum Direktnachweis und zur Kulturbestätigung von Neisseria gonorrhoeae

Hlobil¹,

Kohl²

1991

LaboratoriumsMedizin

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Characteristics of Neisseria gonorrhoeae Strains Isolated on Selective and Nonselective Media

Cited by 9 publications

References 25 publications

Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae

Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae

Differences in nitric oxide steady states between arginine, hypoxanthine, uracil auxotrophs (AHU) and non-AHU strains ofNeisseria gonorrhoeaeduring anaerobic respiration in the presence of nitrite

DNA-Hybridisierung zum Direktnachweis und zur Kulturbestätigung von Neisseria gonorrhoeae

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