Characteristics of O157 versus Non‐O157 Shiga Toxin–Producing<i>Escherichia coli</i>Infections in Minnesota, 2000–2006

Hedican, Erin B.; Medus, Carlota; Besser, John M.; Juni, Billie A.; Koziol, Bonnie; Taylor, Charlott; Smith, Kirk E.

doi:10.1086/600302

Cited by 106 publications

(117 citation statements)

References 26 publications

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“…This finding was previously described by many studies (37)(38)(39)(40). Among them, Hedican et al performed a sentinel surveillance of STEC infections in Minnesota and found 22 coinfected patients among 302 STEC-positive patients (40).…”

Section: Discussionsupporting

confidence: 76%

Comparison of Three Different Methods for Detection of Shiga Toxin-Producing Escherichia coli in a Tertiary Pediatric Care Center

2013

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Shiga toxin-producing Escherichia coli (STEC) is a well-known cause of sporadic and epidemic food-borne gastroenteritis. A low infectious dose, approximately 10 microorganisms, is sufficient to cause disease that may lead to hemolytic-uremic syndrome. The objective of this study was to compare the performances of an in-house real-time PCR, a commercial enzyme immunoassay (EIA) (Premier EHEC; Meridian Bioscience), and culture on sorbitol MacConkey agar for the detection of STEC in a tertiary care pediatric hospital. Of 632 stool samples tested, 21 were positive for STEC. All were detected by PCR, 6 were detected by EIA, and only 5 O157 STEC isolates were identified by culture. Among the 15 specimens falsely negative by EIA, there were 9 Stx1, 2 Stx2, and 4 Stx1 and Stx2 STEC isolates. The latter group included 2 O157 STEC isolates that would have been missed if only EIA had been performed. To our knowledge, this is the first prospective study performed in a pediatric hospital which demonstrates the superiority of PCR over EIA for the detection of STEC. We conclude that PCR is specific and more sensitive than EIA. PCR should be considered for routine use in clinical settings where molecular detection facilities are available. Its lower limit of detection, equivalent to the infectious dose, is an obvious advantage for patient care and public health surveillance.

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Section: Discussionsupporting

confidence: 76%

Comparison of Three Different Methods for Detection of Shiga Toxin-Producing Escherichia coli in a Tertiary Pediatric Care Center

2013

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“…In a work carried out in primates, the intravenous inoculation of stx2 produces HUS symptoms, while administration of the same dose of stx1 did not induce the syndrome (Stearns-Kurosawa et al 2010). Although stx2 is more toxic to humans, stx1 has also been attributed in severe illness such as HUS (Hedican et al 2009). …”

Section: Discussionmentioning

confidence: 99%

“…Also in Spain, the majority of sheep STEC isolates was non--O157, with only 8.7% O157:H7-positive STEC (Oporto et al 2008). O157 STEC isolates are worldwide involved in different disease outbreaks in humans, while in recent years, outbreaks involving non-O157 isolates have been increasingly reported (Hedican et al 2009, Scheutz et al 2011.…”

Section: Discussionmentioning

confidence: 99%

Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

et al. 2015

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“…Epidemiological studies suggest that non-O157 serogroups cause a similar but somewhat less severe range of clinical manifestations than O157 strains (28, 34,35). Retrospective analyses of patient clinical profiles from a U.S. hospital revealed similar incidences of bloody diarrhea and HUS in children presenting with either non-O157 (17 patients) or O157 (33 patients) infections (36).…”

Section: Observations From Human Outbreaksmentioning

confidence: 95%

Animal Models of EnterohemorrhagicEscherichia coliInfection

Ritchie

2014

Microbiol Spectr

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The first major outbreaks caused by enterohemorrhagic Escherichia coli (EHEC) raised public and medical awareness of the risks associated with acquiring this potentially deadly infection. The widespread presence of these organisms in the environment, the severity of the clinical sequelae, and the lack of treatment options and effective preventive measures demand that we obtain a better understanding of how this group of organisms cause disease. Animal models allow study of the processes and factors that contribute to disease and, as such, form a valuable tool in the repertoire of infectious disease researchers. Yet despite more than 30 years of research, it seems that no single model host reproduces the full spectrum of clinical disease induced by EHEC in humans. In the first part of this review, a synopsis of what is known about EHEC infections is garnered from human outbreaks and biopsy specimens. The main features and limitations of EHEC infection models that are based on the three most commonly used species (pigs, rabbits, and mice) are described within a historical context. Recent advances are highlighted, and a brief overview of models based on other species is given. Finally, the impact of the host on moderating EHEC infection is considered in light of growing evidence for the need to consider the biology and virulence strategies of EHEC in the context of its niche within the intestine.

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Characteristics of O157 versus Non‐O157 Shiga Toxin–ProducingEscherichia coliInfections in Minnesota, 2000–2006

Abstract: Differences in severity among STEC infections could not be explained by stx2, suggesting that additional factors are important in STEC virulence.

Cited by 106 publications

References 26 publications

Comparison of Three Different Methods for Detection of Shiga Toxin-Producing Escherichia coli in a Tertiary Pediatric Care Center

Comparison of Three Different Methods for Detection of Shiga Toxin-Producing Escherichia coli in a Tertiary Pediatric Care Center

Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

Animal Models of EnterohemorrhagicEscherichia coliInfection

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