Characteristics of OGTT and Correlation Between the Insulin to C-Peptide Molar Ratio, HOMA-IR, and Insulin Antibodies in T2DM Patients

Abstract

Purpose The purpose of our study was to analyze the characteristics of OGTT and the correlation between the insulin to C-peptide molar ratio (ICPR), HOMA-IR and insulin antibodies (IAs) in T2DM patients. Patients and Methods A total of 77 T2DM patients were included and divided into the IA+ group (25 patients) and IA- group (52 patients). The values of serum glucose, insulin, and C-peptide testing during 2-h OGTT were summarized comparatively, and ROC was made to analyze the predictive value of ICPR for IAs. Results At each time point of OGTT, there was no significant difference in serum glucose and C-peptide changes (p>0.05). Serum insulin levels in positive patients were elevated or not at different time points of the OGTT but ICPR was significantly different (P<0.05) in the two groups. Spearman correlation coefficient analysis showed that the presence of insulin antibodies was correlated with ICPR, but not with HOMA-IR, and ICPR-2h had a better prediction capacity (AUC=0.735, the optimal cutoff-point=0.11, Se=0.760, Sp=0.635). Conclusion T2DM patients with IAs showed no difference in serum glucose and serum C-peptide changes, but elevated or not insulin levels on the OGTTs, compared with negative patients. ICPR-2h can be a preliminary diagnostic index to timely predict IAs in T2DM patients.

The Application of the Insulin to C-Peptide Molar Ratio (ICPR) in Primary Screening for Insulin Antibodies in Type 2 Diabetes Mellitus Patients: A Further Quantitative Study on the Relationship Between ICPR and Insulin Antibodies

“…Based on our previous study, 19 we found that 2h-ICPR in OGTT has a high predictive value for IAs in T2DM patients. What’s more, it is well known that people with high BMI have a significantly increased risk of developing diabetes and that obesity increases the risk of developing type 2 diabetes with a much greater effect than even genetics.…”

“… 18 ICPR is a measure of the endocrine function of the pancreas islets, 15 its association with IAs is not fully supported by clinical evidence, Chinese domestic case populations have received less research, and there is no agreed-upon diagnostic consensus. To quantitative analyze the relationship between ICPR and IAs, our preliminary study 19 used the positive and negative of IA as a dichotomous index, and the diagnostic receiver operating characteristic curve (ROC curve) was used to analyze the predictive value of the ICPR for IA, and the results showed that the area under the ROC curve for 2-hour ICPR (2h-ICPR) prediction of IA in the oral glucose tolerance test (OGTT) was 0.735, (Se=0.760, Sp=0.635, the optimal cut-off point was 0.11), indicating that 2h-ICPR has a good predictive ability for the presence of IAs. When 2h-ICPR>0.11, it indicates that IA is likely to exist in patients, and the higher the value, the higher the possibility of IA positivity in T2DM patients.…”

Irpex lacteus polysaccharide exhibits therapeutic potential for ovarian fibrosis in PCOS rats via the TGF-β1/smad pathway

“…An enzyme-linked immunosorbent assay (ELISA) was used to measure the hormone levels of FSH, E2, LH, T and fasting INS in the serum. The ratio of LH/FSH and homeostasis model assessment-insulin resistance (HOMA-IR) level were calculated (HOMA-IR=FBG (mmol/L) × fasting INS (mU/L)/22.5) [ 30 ]. Serum levels of LDL-C, TC, TG, MDA, SOD and HDL-C were measured using biochemical kits.…”