Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia�belangeri (Wagner, 1841)

Abstract

Tree shrews are most closely related to the primates and so possess a number of advantages in experimental studies; they have been used as an animal model in bacterial and virus infection, cancer, endocrine system disease, and certain nervous system diseases. Their olfactory ensheathing cells (OECs) are able to release several cytokines to promote neuronal survival, regeneration and remyelination. The present study used western blot analysis to identify antibody specificity in protein extracts from whole tree shrew brains to identify the specificity of p75 nerve growth factor receptor (NGFR) derived from rabbits (75 kDa). OECs were cultured and isolated, then stained and identified using the antibodies for p75NGFR. To investigate the capacity of OECs to express cytokines and growth factors, microarray technology was used, and the analysis revealed that OECs were able to express 9,821 genes. Of these genes, 44 genes were from the neurotrophic factor family, which may indicate their potential in transplantation in vivo . The present study considered the function of OECs as revealed by other studies, and may contribute to future research.

Impact of Copper Nanoparticles and Copper Ions on Transcripts Involved in Neural Repair Mechanisms in Rainbow Trout Olfactory Mucosa

“…Following exposure to CuNPs, the OECs altered the olfactory lamina propria microenvironment mostly in favour of axon regeneration. In fact, the expression of neurite outgrowth promoters, which are secreted from OECs, including SERPINE1, SPARC, ADAMTS1, FGFR2, MSLN, and FINC (Lin et al, 2019;Roet et al, 2013), were upregulated (Table 1). However, the expression of two cell adhesion molecules (i.e., NCAM1 and CDH2 (a.k.a., N-CAD)), which can be produced by OEC to promote the axon outgrowth (Rigby et al, 2020), were significantly decreased in the CuNP-exposed fish (Table 1).…”

The great migration: How glial cells could regulate GnRH neuron development and shape adult reproductive life

“…A recent study showed that cytokines modulate olfaction through ensheathing glia on drosophila (Cai et al, 2021). OECs express a wide range of cytokine receptors (Lin et al, 2019); and cytokines induce a metabolic reprogramming of ensheathing glia, thus affecting olfactory neurons (Cai et al, 2021). Previous studies have shown that lipopolysaccharide (LPS)-induced maternal inflammation affects GnRH neuronal development in fetal rodents and induces disruptions of the hypothalamic-pituitary-gonadal axis in pre-and post-pubertal periods (Izvolskaia et al, 2016;Sharova et al, 2015).…”