Characteristics of Plasma Activated Medium Produced by Atmospheric Pressure Helium Plasma Jet and Its Selective Effect on Malignant Melanoma and Normal Fibroblast Cells

Xu, Genhui; Hao, Yanpeng; Sun, Ming-Yang; Liu, Jinren; Xue-yin, Shi; Zhang, Guanjun

doi:10.1109/tps.2020.2965564

Cited by 10 publications

(13 citation statements)

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“…A significantly higher concentration of H 2 O 2 than NO 2 − was also measured in PAM after activating 100 µL DMEM by helium plasma jet for 1 min from 2 cm distance (500 µM H 2 O 2 and 300 µM NO 2 − ) [ 35 ]. 1 mL RPMI medium activated with helium jet for 1 min (U = 12 kV) contained approximately 80 H 2 O 2 and 60 µM NO 2 − [ 36 ]. On the other hand, argon plasma jet with ultra-high density of electrons generated around 17 µM H 2 O 2 and 640 µM NO 2 − in 3 mL DMEM medium after 1 min [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…Different types of human fibroblasts were used in those studies, and it was shown that the treatment with PAM was better tolerated than the direct plasma treatment [ 39 ]. PAM activated by helium plasma jet decreased the Nuff fibroblasts’ viability by 20% [ 40 ] and 3T3 fibroblasts if PAM was activated for more than 30 s [ 36 ]. PAM activated by argon plasma reduced the viability of WI-38 fibroblasts by 40% [ 19 ]; however, it had no effect on mammary epithelial cells [ 10 ] or human astrocytes [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

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Selective Apoptotic Effect of Plasma Activated Liquids on Human Cancer Cell Lines

et al. 2021

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Plasma medicine is a new field focusing on biomedical and clinical applications of cold gas plasmas, including their anticancer effects. Cold plasmas can be applied directly or indirectly as plasma-activated liquids (PAL). The effects of plasma-activated cell growth medium (PAM) and plasma-activated phosphate buffered saline (PAPBS) were tested, using a plasma pen generating streamer corona discharge in ambient air, on different cancer cell lines (melanoma A375, glioblastoma LN229 and pancreatic cancer MiaPaCa-2) and normal cells (human dermal fibroblasts HDFa). The viability reduction and apoptosis induction were detected in all cancer cells after incubation in PAL. In melanoma cells we focused on detailed insights to the apoptotic pathways. The anticancer effects depend on the plasma treatment time or PAL concentration. The first 30 min of incubation in PAL were enough to start processes leading to cell death. In fibroblasts, no apoptosis induction was observed, and only PAPBS, activated for a longer time, slightly decreased their viability. Effects of PAM and PAPBS on cancer cells showed selectivity compared to normal fibroblasts, depending on correctly chosen activation time and PAL concentration, which is very promising for potential clinical applications. This selectivity effect of PAL is conceivably induced by plasma-generated hydrogen peroxide.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Selective Apoptotic Effect of Plasma Activated Liquids on Human Cancer Cell Lines

et al. 2021

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“…Different types of human fibroblasts were used in studies and it was shown that the treatment with PAM was better tolerated than the direct plasma treatment [38]. PAM activated by He plasma jet decreased the Nuff fibroblasts viability by 20% [39] and 3T3 fibroblasts, if PAM was activated for more than 30 seconds [40]. PAM activated by Ar plasma reduced the viability of WI-38 by 40% [26], however, it had no effect on mammary epithelium cells [41] or human astrocytes [24].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Plasma Activated Medium and PBS on Human Melanoma Cells Compared With Other Cancer and Normal Cells

Sersenová¹,

Machala²,

Repiská³

et al. 2021

Preprint

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Plasma medicine is a new field focusing on biomedical and clinical applications of cold physical plasmas, including their anticancer effects. Cold plasmas can be applied directly or indirectly as plasma activated liquids (PAL). The effect of plasma activated cell growth medium (PAM) and plasma activated phosphate buffered saline (PAPBS) were tested using a plasma pen generating streamer corona discharge in ambient air, on different cancer cell lines (melanoma A375, glioblastoma LN229 and pancreatic cancer MiaPaCa-2) and normal cells (human dermal fibroblasts HDFa). The viability reduction and apoptosis induction were detected in all cancer cells after incubation in PAL. In melanoma cells we focused on detailed insights to the apoptotic pathways. The anticancer effects depend on the plasma treatment time or PAL concentration. The first 30 minutes of incubation in PAL were enough to start processes leading to the cell death. In fibroblasts, no apoptosis induction was observed, only PAPBS, activated for longer time, slightly decreased their viability. Anticancer effects of PAM and PAPBS on cancer cells showed selectivity compared to normal fibroblasts, depended on correctly chosen activation time and PAL concentration. This selectivity, supported by optimum ratio of hydrogen peroxide and nitrites in PAL, is very promising for potential clinical applications.

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“…Comparison of H2O2 production with similar plasma sou sented in Figure 8. Our results were compared to other sources that operate region: 39.5 kHz (marked as a circle [22]), 30 kHz (marked as triangle [9]) (marked as square [4]). Here, other concentrations are quite high compared to One possible reason for this difference in H2O2 concentrations could be various media that were used; MEM was used in our research, DMEM (Dulbecco's Mo Medium) was used in [9] and RPMI 1640 was used in [22].…”

Section: Discussionmentioning

confidence: 99%

“…Our results were compared to other sources that operate region: 39.5 kHz (marked as a circle [22]), 30 kHz (marked as triangle [9]) (marked as square [4]). Here, other concentrations are quite high compared to One possible reason for this difference in H2O2 concentrations could be various media that were used; MEM was used in our research, DMEM (Dulbecco's Mo Medium) was used in [9] and RPMI 1640 was used in [22]. However, the resul inside the same medium (DMEM), different concentrations of hydrogen pero obtained-almost a threefold difference for the longest treatment time of 60 conclude that the generation of H2O2 is not the main source of our plasma [16], the triangle is [7], the square is [5] and the star represents our system.…”

Section: Discussionmentioning

confidence: 99%

Analysing Mouse Skin Cell Behaviour under a Non-Thermal kHz Plasma Jet

et al. 2021

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Plasma jets are extensively used in biomedical applications, particularly for exploring cell viability behaviour. However, many experimental parameters influence the results, including jet characteristics, secondary liquid chemistry and protocols used, slowing research progress. A specific interest of the presented research was skin cell behaviour under a non-thermal kHz plasma jet—a so-called cold plasma jet—as a topical skin treatment. Our research was focused on in vitro mouse skin cell direct plasma treatment with argon as an operating gas. The research was complemented with detailed gas-phase diagnostics and liquid-phase chemical analysis of the plasma and plasma-treated medium, respectively. The obtained results showed that direct plasma jet treatment was very destructive, leading to low cell viability. Even with short treatment times (from 35 s to 60 s), apoptosis was observed for most L929 murine fibroblasts under approximately the same conditions. This behaviour was attributed to plasma species generated from direct treatment and the types of cell lines used. Importantly, the research exposed important points that should be taken under consideration for all further research in this field: the urgent need to upgrade and standardise existing plasma treatment protocols of cell lines; to monitor gas and liquid chemistries and to standardise plasma discharge parameters.

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Characteristics of Plasma Activated Medium Produced by Atmospheric Pressure Helium Plasma Jet and Its Selective Effect on Malignant Melanoma and Normal Fibroblast Cells

Cited by 10 publications

References 50 publications

Selective Apoptotic Effect of Plasma Activated Liquids on Human Cancer Cell Lines

Selective Apoptotic Effect of Plasma Activated Liquids on Human Cancer Cell Lines

The Effect of Plasma Activated Medium and PBS on Human Melanoma Cells Compared With Other Cancer and Normal Cells

Analysing Mouse Skin Cell Behaviour under a Non-Thermal kHz Plasma Jet

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