Characteristics of Progenitor Cells Derived from Adult Ciliary Body in Mouse, Rat, and Human Eyes

Xu, Huixiong; Iglesia, Drina D. Sta.; Kielczewski, Jennifer L.; Valenta, Danielle; Pease, Mary Ellen; Zack, Donald J.; Quigley, Harry A.

doi:10.1167/iovs.06-1034

Cited by 75 publications

(66 citation statements)

References 35 publications

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“…The expression of Oct4 and Pax6 indicated dedifferentiation of the cells from the spheres under the effect of factors of the medium. Presumably, some of them possessed properties of poorly differentiated cells, characterized by expression of Pax6, essential for maintaining the proliferation and differentiation [15,24]. Multipotency of cells from the spheres manifested by their development in the neuronal and epithelial direction.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Phenotypic Modifi cation of Pigmented Epithelium Cells from Human Eye at Early Stages of Development

et al. 2009

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Multipotent characteristics of human fetal (9-11.5 weeks) pigmented epithelial retinal cells and capacity to transdifferentiation in neuronal direction were studied in vitro under different culturing conditions. The cultures were analyzed using a wide spectrum of antibodies. It was found that pigmented epithelium of human eye is a heterogeneous cell population with three subtypes differing by adhesion characteristics, migration, transdifferentiation potential, and reaction to microenvironmental factors. Subtype 1 cells steadily retain their epithelial characteristics, subtype 2 cells change their morphotype and produce neuroblast and photoreceptor cell proteins, and subtype 3 cells form free floating spheres and are capable to multipotent differentiation.

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Section: Resultsmentioning

confidence: 99%

In Vitro Phenotypic Modifi cation of Pigmented Epithelium Cells from Human Eye at Early Stages of Development

et al. 2009

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“…Importantly, the retina may also continue to grow after birth and new cells may be generated during lifespan after injury due to the presence of retinal stem cell (RSCs) resident in a region adjacent to the retina, the ciliary epithelium (CE) located in the pigmented ciliary bodies of the adult mammalian eye (Fig. 3) [11,16,[170][171][172][173]. These multipotent RSCs located in the pigmented ciliary bodies, also designated CE stem cells, express several stem cell markers, including telomerase, neural markers such as nestin, and retinal progenitor markers such as Pax 6 [170][171][172].…”

Section: Ocular Stem Cellsmentioning

confidence: 99%

Recent Progress on Tissue-Resident Adult Stem Cell Biology and Their Therapeutic Implications

2008

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Recent progress in the field of the stem cell research has given new hopes to treat and even cure diverse degenerative disorders and incurable diseases in human. Particularly, the identification of a rare population of adult stem cells in the most tissues/organs in human has emerged as an attractive source of multipotent stem/progenitor cells for cell replacement-based therapies and tissue engineering in regenerative medicine. The tissue-resident adult stem/progenitor cells offer the possibility to stimulate their in vivo differentiation or to use their ex vivo expanded progenies for cell replacement-based therapies with multiple applications in human. Among the human diseases that could be treated by the stem cell-based therapies, there are hematopoietic and immune disorders, multiple degenerative disorders, such as Parkinson's and Alzeimeher's diseases, type 1 or 2 diabetes mellitus as well as eye, liver, lung, skin and cardiovascular disorders and aggressive and metastatic cancers. In addition, the genetically-modified adult stem/progenitor cells could also be used as delivery system for expressing the therapeutic molecules in specific damaged areas of different tissues. Recent advances in cancer stem/progenitor cell research also offer the possibility to targeting these undifferentiated and malignant cells that provide critical functions in cancer initiation and progression and disease relapse for treating the patients diagnosed with the advanced and metastatic cancers which remain incurable in the clinics with the current therapies.

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“…These findings have led to the hypothesis that the adult retina might regenerate during life in other species besides fish and amphibians. Retinal stem cells have also been found in vitro in the ciliary body (CB) [14,15] and the iris [15]. On the one hand, it has been shown that most precursor cells in the ciliary margin may give rise to both epithelial cells and neurons [16,17].…”

Section: Introductionmentioning

confidence: 99%

Proliferation of the ciliary epithelium with retinal neuronal and photoreceptor cell differentiation in human eyes with retinal detachment and proliferative vitreoretinopathy

Ducournau

Boscher

Adelman

et al. 2011

Graefes Arch Clin Exp Ophthalmol

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Our observations suggest that EGFR-positive CE cells in the adult human eye in vivo with RD and PVR form "neurosphere-like" structures; their differentiation seems to be directed towards the neural and photoreceptor lineage, and not towards glial formation. In the adult human eye, the CE in a pathological retinal environment such as RD might provide a spontaneous source of donor cells for retinal transplantation.

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Characteristics of Progenitor Cells Derived from Adult Ciliary Body in Mouse, Rat, and Human Eyes

Cited by 75 publications

References 35 publications

In Vitro Phenotypic Modifi cation of Pigmented Epithelium Cells from Human Eye at Early Stages of Development

In Vitro Phenotypic Modifi cation of Pigmented Epithelium Cells from Human Eye at Early Stages of Development

Recent Progress on Tissue-Resident Adult Stem Cell Biology and Their Therapeutic Implications

Proliferation of the ciliary epithelium with retinal neuronal and photoreceptor cell differentiation in human eyes with retinal detachment and proliferative vitreoretinopathy

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