Characteristics of Release Pattern of Somatostatin and Glucagon of Pancreatic Islets Cultured in Glucose and Adenosine

Yoshioka, Masako; Taniguchi, H; Tsutou, Akimitsu; Murakami, Keiji; Ejiri, Kazushige; Ishihara, Kenzo; Utsumi, M.; Morita, Soichiro; Baba, Shigeaki

doi:10.1055/s-2007-1018736

Horm Metab Res

1983

DOI: 10.1055/s-2007-1018736

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Characteristics of Release Pattern of Somatostatin and Glucagon of Pancreatic Islets Cultured in Glucose and Adenosine

Masako Yoshioka¹,

H Taniguchi²,

Akimitsu Tsutou³

et al.

Abstract: The present authors reported that insulin release from cultured rat: pancreatic islets could be maintained for 27 days by raising the glucose concentration or by adding one of purine nucleosides, adeno sine, in culture medium (Tsutou 1980; Taniguchi, Tsutou and Baba 1980). However, the glucagon and somatostatin release from cul tured islets during a long period, more than 3 days, has not been reported (

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1987

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Insulin, glucagon and somatostatin secretion by cultured islets from normal and diabetic hamsters

Shieh

Dunbar

1987

Acta diabet. lat

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The interhormonal relationship within the pancreatic islets have been studied by previous investigators, but the cellular interplay and the sequence of events in the islet cell's response to stimulators has remained unclear. In the present study, pancreatic islets were isolated by collagenase digestion from normal and streptozotocin-diabetic hamsters the latter being maintained with insulin treatment. The diabetic animals were used to provide A- and B-cell enriched islets. The islets from normal and diabetic hamsters were cultured in medium 199 plus 10% fetal calf serum with 0.8 or 5 mg/ml glucose. The cultures were maintained for up to seven days with medium changes every third day. At specified intervals, media were collected and assayed for insulin, glucagon and somatostatin. Our results showed the expected increased insulin secretion by the B-cells in response to high glucose. However, after two days of culture accumulative insulin secretory response was reduced and at the end of seven days was less than the insulin produced in low glucose medium. Glucagon secretion by the A-cells was similar for low and high glucose media for the entire culture period. Somatostatin secretion by D-cells was stimulated by high glucose but was attenuated after 2 days. No correlation could be found between the concentration of hormone in the media and a possible effect on a specific islet secretion. However, the fact that insulin secretion by islets cultured in high glucose was decreased after two days may indicate a refractoriness produced by persistent hyperglycemia. Islets isolated from diabetic animals secreted more glucagon and less insulin than control islets. Somatostatin secretion was the same in both groups. It was concluded that paracrine relationships were relatively insignificant in the regulation of islet secretion in a prolonged culture environment and persistent high glucose reduced the B-cell response to glucose stimulation.

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Insulin, glucagon and somatostatin secretion by cultured islets from normal and diabetic hamsters

Shieh

Dunbar

1987

Acta diabet. lat

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show abstract

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Characteristics of Release Pattern of Somatostatin and Glucagon of Pancreatic Islets Cultured in Glucose and Adenosine

Cited by 1 publication

References 6 publications

Insulin, glucagon and somatostatin secretion by cultured islets from normal and diabetic hamsters

Insulin, glucagon and somatostatin secretion by cultured islets from normal and diabetic hamsters

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