Characteristics of rhizobia isolated from three legumes indigenous to the Canadian high arctic:Astragalus alpinus, Oxytropis maydelliana, andOxytropis arctobia

Prévost, Danielle; Bordeleau, L. M.; Caudry-Reznick, Suzanne; Schulman, Herbert M.; Antoun, Hani

doi:10.1007/bf02378352

Cited by 68 publications

(53 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The fact that the values of nodule SARA in all the plots decreased similarly during this period suggests that there were no qualitative differences in the nodule resistance to the low temperature among the different treatments. Prevost et al (1987) demonstrated that Rhizobium in liquid culture, which had been isolated from nodules of Astragalus alpinus growing in the Canadian high arctic zone could grow at 5~ According to the present results showing that the nodules were still able to grow in winter, it is suggested that the multiplication and development of bacteria or bacteroids inside the infected cells of Chinese milk vetch nodules did not cease even under the low temperature conditions. As might be expected from our previous study indicating that SARA values generally showed a positive correlation with the nodule N concentration, the decrease of the values of nodule ARA and SARA in all the plots during the low temperature period was closely associated with a sharp decrease of the nodule N concentration.…”

Section: Discussionmentioning

confidence: 49%

Effect ofRhizobiuminoculation and application of N, P, K fertilizer on the growth and nitrogen fixation of field-grown Chinese milk vetch

Arima

1992

Soil Science and Plant Nutrition

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 49%

Effect ofRhizobiuminoculation and application of N, P, K fertilizer on the growth and nitrogen fixation of field-grown Chinese milk vetch

Arima

1992

Soil Science and Plant Nutrition

View full text Add to dashboard Cite

“…There are several Rhizobium species recognized at this time (10, 1.3, 24), and knowledge of rhizobial evolution and phylogeny obtained in recent studies allows the proposal of new rhizobial taxa (3,16,25). However, the current systematic description of the genus Rhizobium does not include many of the root nodule isolates from legumes of tropical (22,37), arctic (21), and temperate (5,12,17) regions.…”

mentioning

confidence: 99%

Numerical Taxonomy of Rhizobium Strains from Legumes of the Temperate Zone

Novikova¹,

Павлова²,

Vorobjev³

et al. 1994

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

Phenotypic properties (growth characteristics, utilization of carbon and nitrogen sources, and intrinsic antibiotic resistance) of 53 Rhizobium strains isolated from root nodules of the temperate-zone legumes Astragalus spp. (29 strains), Oxytropis campanuhta (7 strains), Hedysarum alpinum (7 strains), Ononis awensis (3 strains), Glycyrrhiza spp. (4 strains), and Coronilh varia (3 strains) were compared with those of other Rhizobium, Bradyrhizobium, and Agrobacterium strains. The final matrix, containing 83 strains and 56 nonsymbiotic features, was used for computer cluster analysis. The dendrogram showed that the new strains of temperate-zone rhizobia formed a cluster separate from both Rhizobium and Bradyrhizobium spp. Two large groups of temperate-zone rhizobia were revealed. Group 1 included rhizobial strains originating from different geographical regions with a temperate climate, while group 2 included strains from the same geographical origin, South Siberia. The 'strains of recognized Rhizobium species were clustered, in general, with each other as expected from phylogenetic relatedness.Root nodule bacteria of the genera Rhizobium and Bradyrhizobium are a common microflora of temperate soils. These bacteria may interact with the roots of leguminous plants, inducing the formation of nodules in which the fixation of atmospheric nitrogen occurs. These bacteria are commonly used in agriculture for improvement of plant growth. The specificity of the interaction between rhizobia and legumes depends on many factors coded for by the host cultivar and bacterial genomes;. The main properties of a bacterial isolate can be described by its taxonomical position in relation to other strains. There are several Rhizobium species recognized at this time (10, 1.3, 24), and knowledge of rhizobial evolution and phylogeny obtained in recent studies allows the proposal of new rhizobial taxa (3,16,25). However, the current systematic description of the genus Rhizobium does not include many of the root nodule isolates from legumes of tropical (22, 37), arctic (21), and temperate (5, 12, 17) regions.The host plant specificities and phage sensitivities of Rhizobium strains from temperate-zone legume root nodules formed on Astragalus, Hedysamm, Glycywhiza, and Ononis plants were recently described (17). It was shown that these strains constitute a cross-inoculation group related to Rhizobium loti. The aim of the present work was to determine the cultural properties of these strains and to compare these properties with those of other Rhizobium species. MATERIALS AND METHODSBacterial strains. Bacterial strains used in this work are listed in Table 1. Bacteria were maintained on yeast extractmannitol (YEM) (29) agar slants at 4°C.Growth characteristics. The time necessary for full colony development on YEM agar (estimated here as a colony diameter of 2 mm) was determined. Bacterial growth on meat-peptone agar and reactions with litmus milk were determined as described earlier (11). The ability of strains to grow in the presence of 0,1...

show abstract

“…The proton resonances were close to those observed previously for S. meliloti and R. leguminosarum bv. viciae Nod factors, which are both 6-O acetylated, although on another glucosaminyl residue (15,26): H 6Ј -4.43 ppm, dd, J ϭ 12 and 2.5 Hz; H 6 -4.36 ppm, dd, J ϭ 12 and 1.5 Hz; H 5 -4.16 ppm, m.…”

Section: Resultsmentioning

confidence: 99%

“…For example, Mesorhizobium sp. strain N33, isolated from Oxytropis arctobia, also nodulates Astragalus alpinus and Onobrychis viciifolia (19,26). In contrast, Sinorhizobium meliloti efficiently nodulates alfalfa (Medicago sativa) as well as Melilotus and Trigonella species.…”

mentioning

confidence: 99%

Unusual Methyl-Branched α,β-Unsaturated Acyl Chain Substitutions in the Nod Factors of an Arctic Rhizobium, Mesorhizobium sp. Strain N33 ( Oxytropis arctobia )

et al. 2001

View full text Add to dashboard Cite

Mesorhizobium sp. strain N33 (Oxytropis arctobia), a rhizobial strain isolated in arctic Canada, is able to fix nitrogen at very low temperatures in association with a few arctic legume species belonging to the genera Astragalus, Onobrychis, and Oxytropis. Using mass spectrometry and nuclear magnetic resonance spectroscopy, we have determined the structure of N33 Nod factors, which are major determinants of nodulation. They are pentameric lipochito-oligosaccharides 6-O sulfated at the reducing end and exhibit other original substitutions: 6-O acetylation of the glucosamine residue next to the nonreducing terminal glucosamine and N acylation of the nonreducing terminal glucosamine by methyl-branched acyl chains of the iso series, some of which are ␣,␤ unsaturated. These unusual substitutions may contribute to the peculiar host range of N33. Analysis of N33 whole-cell fatty acids indicated that synthesis of the methyl-branched fatty acids depended on the induction of bacteria by plant flavonoids, suggesting a specific role for these fatty acids in the signaling process between the plant and the bacteria. Synthesis of the methyl-branched ␣,␤-unsaturated fatty acids required a functional nodE gene.Rhizobia are soil bacteria, now classified in several genera (e.g., Rhizobium, Sinorhizobium, Mesorhizobium, Bradyrhizobium, Azorhizobium), which form a symbiotic association with legume plants. The interaction between bacteria and plants results in the formation of nodules on the host plant roots, in which rhizobia fix atmospheric nitrogen. The association between rhizobia and legumes is specific: each rhizobial strain has a defined host range. For example, Mesorhizobium sp. strain N33, isolated from Oxytropis arctobia, also nodulates Astragalus alpinus and Onobrychis viciifolia (19,26). In contrast, Sinorhizobium meliloti efficiently nodulates alfalfa (Medicago sativa) as well as Melilotus and Trigonella species. Mesorhizobium sp. strain N33 was isolated in the Canadian high arctic and is able to grow and fix nitrogen at temperatures as low as 5°C. In addition, it was shown that arctic rhizobia promoted better growth of O. viciifolia at low temperatures than did temperate strains (27). Thus, it might be valuable to extend the host range of arctic rhizobia to agronomically important legumes, in order to improve nitrogen fixation by these plants at low temperatures. It is therefore important to understand the molecular mechanisms controlling the nodulation specificity of arctic rhizobia.Earlier work has shown that nodulation and host specificity in rhizobium-legume symbiosis are determined by signal exchanges between the bacteria and the host plant (21). Flavonoids excreted by the plant roots induce the expression of rhizobial nodulation (nod) genes. Most of these genes are involved in the biosynthesis and secretion of bacterial signals, the Nod factors, that can specifically induce symbiotic responses of the host plants. These responses include root hair deformation, division of root cortical cells and, in some instances,...

show abstract

Characteristics of rhizobia isolated from three legumes indigenous to the Canadian high arctic:Astragalus alpinus, Oxytropis maydelliana, andOxytropis arctobia

Cited by 68 publications

References 16 publications

Effect ofRhizobiuminoculation and application of N, P, K fertilizer on the growth and nitrogen fixation of field-grown Chinese milk vetch

Effect ofRhizobiuminoculation and application of N, P, K fertilizer on the growth and nitrogen fixation of field-grown Chinese milk vetch

Numerical Taxonomy of Rhizobium Strains from Legumes of the Temperate Zone

Unusual Methyl-Branched α,β-Unsaturated Acyl Chain Substitutions in the Nod Factors of an Arctic Rhizobium, Mesorhizobium sp. Strain N33 ( Oxytropis arctobia )

Contact Info

Product

Resources

About