Characteristics of self-alkalization in high-rate denitrifying automatic circulation (DAC) reactor fed with methanol and sodium acetate

Li, Wei; Zheng, Ping; Guo, Ji-Ming; Ji, Junyuan; Zhang, Meng; Zhang, Zonghe; Zhan, En-chao; Abbas, Ghulam

doi:10.1016/j.biortech.2013.11.097

Cited by 59 publications

(12 citation statements)

References 22 publications

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“…Experiments were performed in a modified plexiglass-made upflow sludge bed (UASB) reactor with an effective volume of 1.25 L (height and inner diameter of 45 and 6 cm, respectively). 17 Anearobic granular sludge (AnGS) used for denitratation enrichment was taken from a full-scale wastewater treatment system at a paper manufactur-ing facility (Zhejiang Paper JSC Ltd. (30.42°N, 120.57°E), China). The description of the inoculation sites is available in the Support Information (SI).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Salinity-Aided Selection of Progressive Onset Denitrifiers as a Means of Providing Nitrite for Anammox

Liu

et al. 2018

Environ. Sci. Technol.

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Anaerobic ammonium oxidation (anammox) combined with partial-denitrification (NO → NO) is an innovative process for the simultaneous removal of ammonia and nitrate from wastewaters. An efficient method for the selection of partial denitrifying community, which relies on increasing influent salinity, is described. Using this method, a denitratating community was enriched, which showed a nitrite accumulation efficiency higher than 75% as well as a high nitrate conversion efficiency. Community analysis using 16S rDNA indicated that Halomonas became the dominant genus as salinity increased. Metagenomic analysis revealed that there was not a significant difference in reads mapping to downstream denitrification genes in a comparison of samples from cultures with 5% salinity to those without salinity. The majority of the reads mapping to the genes encoding dissimilatory nitrate and nitrite reductases nar and nirS came from Halomonas under high salinity conditions. Two metagenome-assembled genomes taxonomically assigned to Halomonas were obtained, one of which accounted for ∼35% of the reads under high salinity conditions. Both genomes harbored the genes for the complete denitrification pathway. These results indicate progressive onset denitrifiers, a phenotype where nitrite reduction only occurs after nitrate exhaustion, could be successfully enriched with increasing salinity. Progressive onset denitrifiers may be more widespread in natural and artificial habitats than anticipated and are shown here to be valuable for nitrogen mitigating processes.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Salinity-Aided Selection of Progressive Onset Denitrifiers as a Means of Providing Nitrite for Anammox

Liu

et al. 2018

Environ. Sci. Technol.

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“…The carbonate equilibrium was thus strongly involved in the pH regulation. Other works dealing with granular sludge have highlighted the opposite, with a pH increase from 7.0 in the reactor influent to 9.3 in the effluent (Li et al, 2014(Li et al, , 2015. This behaviour is called "self alkalinisation" because of the consumption of hydroxide ions by the denitrification process.…”

Section: Continuous Supply Of Standard Mediummentioning

confidence: 99%

Nitrate and nitrite reduction at high pH in a cementitious environment by a microbial microcosm

Durban

Rafrafi

Rizoulis

et al. 2018

International Biodeterioration & Biodegradation

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The possible release of oxyanions, such as nitrate, from radioactive waste repositories may influence redoxconditions of the near field environment and thus promote mobility of some redox sensitive radionuclides. The fate of dissolved oxyanions will be significantly conditioned by microbial activities, if present in the aqueous interstitial phase of a waste cell. This study investigates microbial nitrate reduction in a cementitious environment. A consortium of microorganisms was used, an inoculum prepared with sediments collected from a former lime works site, characterized by a pH of pore water of 11-12. The biomass was acclimated to cement leachate supplemented with nitrate, acetate and yeast extract. According to experiments performed in closed and in dynamic systems, the microbial consortium was adapted to reduce nitrate and nitrite in a cementitious, anaerobic environment (pH 11, with and without hardened cement paste and leachate). Although, nitrite accumulation was observed in close system and temporally in dynamic system. The rate of nitrate reduction was between 0.12 and 0.75 mM/h with incoming nitrate concentrations between 6 and 48 mM, respectively. The microorganism diversity and the biofilm present on the hardened cement paste helped maintain microbial activity in all of the conditions simulating cementitious environments.

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“…Therefore, according to the initial pH, two opposite pH evolutions were observed: acidification starting from pH 10.8 and alkalinisation at pH < 9.5. Similarly, in the literature, 'self-acidification' at high pH of 10.0 or 11.0 (Mateju et al 1992;Glass and Silverstein 1998;Durban et al 2018) and 'self-alkalinisation' at pH 7.0 (Li et al 2014(Li et al , 2015 have been reported in denitrifying cultures.…”

Section: Discussionmentioning

confidence: 59%

Adaptation of neutrophilic Paracoccus denitrificans to denitrification at highly alkaline pH

Albina

Durban

Bertron

et al. 2020

Environ Sci Pollut Res

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Bacterial denitrification is widely documented at neutral pH in order to improve the removal of nitrate in wastewater treatment processes. However, certain industrial contexts generate alkaline waste and effluent containing nitrate that must be denitrified. To obtain more information on denitrification at alkaline pH, this study evaluated the possibility of adapting a neutrophilic denitrifying strain, Paracoccus denitrificans, to alkaline pH. Firstly, P. denitrificans' denitrifying activity was evaluated without acclimation in batch bioreactors at pH 7.0, 8.0, 9.0 and 10.0. Then, two acclimation methods using successive batch bioreactors and a continuous bioreactor allowed P. denitrificans to be gradually exposed to alkaline pH: from 8.5 to 11.2 in 26 and 72 days respectively. Results showed that P. denitrificans could grow and catalyse nitrate reduction (i) at pH 9.0 without acclimation, (ii) at pH 10.5 in successive batch cultures with progressively increasing pH and (iii) at pH 10.8 in continuously fed culture with a hydraulic retention time (HRT) of 8 days. It was shown that denitrification affected the pH despite the presence of carbonate buffering of the P. denitrificans growth medium. With acetate as an electron donor, the pH of a carbonate buffered medium tends towards pH 10 during the process of denitrification. Keywords Nitrate reduction . High pH . Denitrifying bacteria . Acclimation . Batch and continuous bioreactors Highlights • First demonstration of denitrification with P. denitrificans at alkaline pH (> 9.5) • Comparison of culture-dependent methods for bacterial acclimation • P. denitrificans survived temporarily to pH 11.2 in a continuous bioreactor • Hydraulic retention time of 8 days allowed P. denitrificans to adapt to pH 10.8

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Characteristics of self-alkalization in high-rate denitrifying automatic circulation (DAC) reactor fed with methanol and sodium acetate

Cited by 59 publications

References 22 publications

Salinity-Aided Selection of Progressive Onset Denitrifiers as a Means of Providing Nitrite for Anammox

Salinity-Aided Selection of Progressive Onset Denitrifiers as a Means of Providing Nitrite for Anammox

Nitrate and nitrite reduction at high pH in a cementitious environment by a microbial microcosm

Adaptation of neutrophilic Paracoccus denitrificans to denitrification at highly alkaline pH

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