Characteristics of spectrin-induced leakage of extruded, phosphatidylserine vesicles

Structure of three Humanin peptides with different activities upon interaction with liposome

“…Unilamellar liposomes with DOPC or DOPG were prepared by the lipid extrusion protocol as described previously [33,34]. Briefly, the lipids dissolved in chloroform were placed in a round glass vial and subjected to evaporation in a vacuum desiccator over 12 h to remove the solvent.…”

pH-dependent Interactions of the Carboxyl-terminal Helix of Steroidogenic Acute Regulatory Protein with Synthetic Membranes

“…SUVs were generated by equilibrating the lipid solution with an extrusion apparatus (Avanti Polar Lipids, part number 610000) at 37°C for 10 min and passing the solution through a 0.1-m polycarbonate membrane 21 times (22,23). An average particle size of 100 Å was confirmed by dynamic light scattering, and vesicles were used within 24 h of preparation.…”

Effect of phospholipids on conformational structure of bovine pancreatic trypsin inhibitor (BPTI) and its thermolabile mutants

“…1,2‐Dipalmitoyl‐rac‐glycero‐3‐phosphocholine (PC) and 1,2‐dipalmitoyl‐rac‐glycero‐3‐phospho‐ L ‐serine (PS) were purchased from Sigma‐Aldrich Co. (Tokyo, Japan). To obtain large and homogeneous unilamellar vesicles (LUV), we used a lipid extrusion protocol as described by previous reports 19, 20. Briefly, the lipid was hydrated in 20 m M sodium phosphate buffer, pH 7.0 (PBS) for 1 h, vortexed, and the resulting dispersions were extruded through a polycarbonate filter (pore size of 100 nm), 20 times, using a Mini Extruder (Avanti Polar Lipids, Inc., Alabaster, AL).…”